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Ontogenic study of Expression of IGF-I and Gh-Receptor (GHR) mRNA in pig Liver and Skeletal Muscle.

Published online by Cambridge University Press:  24 November 2017

J.M. Brameld ,
P.A. Weller ,
R.S. Gilmour  and
P.J. Buttery
J.M. Brameld
Affiliation:
University of Nottingham, School of Agricultural and Food Sciences, Sutton Bonington, Loughborough LE12 5RD
P.A. Weller
Affiliation:
AFRC IAPGR, Cambridge Research Station, Babraham, Cambridge CB2 4AT
R.S. Gilmour
Affiliation:
AFRC IAPGR, Cambridge Research Station, Babraham, Cambridge CB2 4AT
P.J. Buttery
Affiliation:
University of Nottingham, School of Agricultural and Food Sciences, Sutton Bonington, Loughborough LE12 5RD
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Extract

Many of the effects of growth hormone are now thought to be mediated via the stimulation of insulin-like growth factor-I (IGF-I) production by many tissues, especially the liver, with this stimulation being dependent upon the presence of the GH-receptor (GHR). IGF-I gene expression occurs via alternative promoters giving rise to class 1 and 2 transcripts, of which class 2 is thought to be preferentially responsive to GH (Saunders, Dickson, Pell & Gilmour, 1991).The effects of IGF-I include the stimulation of DNA synthesis (mitogenesis) and protein synthesis in most cell types, together with the differentiation of many cell types into mature tissue, including the differentiation of muscle cells into muscle fibres. Thus the GH/IGF-I axis has been found to play a major part in the control of animal growth. For this reason, we studied the age related changes in IGF-I and GHR mRNA expression in pig liver and skeletal muscle.

Type
The Application of Molecular Biology to Animal Science
Information
Proceedings of the British Society of Animal Production (1972) , Volume 1993: Winter meeting , March 1993 , pp. 151
Copyright
Copyright © The British Society of Animal Production 1993

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References

Chomczynski, P & Saachi, N. (1989) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162 : 156–159.Google Scholar
Saunders, J.C, Dickson, M.C, Pell, J.M. & Gilmour, R.S. (1991) Expression of a growth hormone-responsive exon of the ovine insulin-like growth factor-I gene. Journal of Molecular Endocrinology 7 : 233–240.Google Scholar