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Effect of Cellulase Enzymes on Degradation Characteristics of Ensiled Rice Straw

Published online by Cambridge University Press:  22 November 2017

Y. Nakashima
Affiliation:
Rowett Research Institute, Bucksburn, ABERDEEN, AB2 9SB
E.R. Ørskov
Affiliation:
Rowett Research Institute, Bucksburn, ABERDEEN, AB2 9SB
K. Ambo
Affiliation:
Rowett Research Institute, Bucksburn, ABERDEEN, AB2 9SB
Y. Takase
Affiliation:
Rowett Research Institute, Bucksburn, ABERDEEN, AB2 9SB
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Abstract

Rice straw was ensiled in laboratory containers of 11 capacity. Three concentrations of moisture (50, 60 or 70%) 3 concentrations of a commercial cellulase enzyme preparation (0, 5 or 10g/kg dry matter (DM)) and 3 types of straw processing (2 cm, 5 mm or 2 mm length) were used. The preparations were stored at room temperature (approximately 20°C) for 30 days. The straw treated with cellulase had a lower final pH (5.21, 4.87, 4.82; P<0.05), higher concentrations of lactic acid (198, 383, 367 mg/100g; P<0.05), a lower content of neutral detergent fibre (689, 630, 620 g/kg DM; P<0.05) and a higher solubility, measured as washing losses from nylon bags (152, 196, 212 g/kg DM; P<0.05) for the 0, 5 and 10 g/kg cellulase treatments respectively. The samples were subsequently incubated in nylon bags in the rumen of 3 sheep for 8, 16, 24, 48 and 72 h to estimate degradation rate and potential degradability using the expression p+a+b(1-e-ct) where p is degradability at time t and a, b and c are constants. While there was no effect of moisture content or physical form of the straw, the degradation rate constant (c) was significantly increased (P<0.05) by the addition of cellulase. The maximum potential (a+b) however was unchanged. The values for c were 0.0496, 0.0677 and 0.0847/h-1 and the values for (a+b) were 624, 621 and 628 g/kg for the 0, 5 and 10 g/kg cellulase enzyme additions respectively. It is concluded that the use of cellulase enzymes can assist in the preservation of wet straw and can result in improved degradation characteristics.

Type
General
Copyright
Copyright © The British Society of Animal Production 1988

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References

Ørskov, E.R. & MacDonald, I. 1979. The estimation of protein degradability in the rumen from incubation measurements weighed according to rate of passage. Journal of Agriculture Science, Cambridge 92: 199502.Google Scholar