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Heavy metal analysis in lens and aqueous humor of cataract patients by total reflection X-ray fluorescence spectrometry

Published online by Cambridge University Press:  24 April 2014

Martina Schmeling ,
Bruce I. Gaynes  and
Susanne Tidow-Kebritchi
Martina Schmeling*
Affiliation:
Loyola University Chicago, Chicago, Illinois 60660
Bruce I. Gaynes
Affiliation:
Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois 60153
Susanne Tidow-Kebritchi
Affiliation:
Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois 60153
*
a)Author to whom correspondence should be addressed. Electronic mail: [email protected]
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Abstract

The human eye is continuously exposed to the environment yet little is known about how much of toxins, specifically heavy metals are present in its different parts and how they influence vision and acuity. To shed light into this subject, aqueous humor and lens samples were collected from 14 cataract patients to study the presence and concentration of selected metals in the eye. Subjects undergoing routine cataract surgery were consecutively enrolled for study by simple random sampling. Prior to surgery, subject demographic were compiled. The surgical procedure involved small incision cataract removal using phacoemulsification. During the procedure, a small aliquot of aqueous humor was retained for analysis, whereas homogenized lens fragments were obtained during phacoemulsification. A balanced salt solution was used as control for each set of samples. Both ocular specimens were analyzed by total reflection X-ray fluorescence spectrometry after dilution and addition of an internal standard. The data obtained show substantial variations in elemental signature between the two media (aqueous humor and lens) and the patients themselves. Most commonly found heavy metals in both types of media were chromium and manganese. Barium was found in the lens, but not in aqueous tissue, whereas nickel was found only in the aqueous humor. Concentrations were generally higher in aqueous samples. Further study and increased sample size are required to more accurately elucidate the relationship between systemic and ocular metal accumulation and the impact of metal accumulation on measures of visual function and ocular disease.

Type
Technical Articles
Information
Powder Diffraction , Volume 29 , Issue 2 , June 2014 , pp. 155 - 158
Copyright
Copyright © International Centre for Diffraction Data 2014 

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References

Erie, J. C., Butz, J. A., Good, J. A., Erie, E. A., Burritt, M. F., and Cameron, J. D. (2005). “Heavy metal concentrations in human eyes,” Am. J. Ophthalmol. 139(5), 888–893.Google Scholar
Guidotti, T. L., McNamara, J., and Moses, M. S. (2008). “The interpretation of trace element analysis in body fluids,” Indian J. Med. Res. 128, 524–532.Google ScholarPubMed
Hernandez-Caraballo, E. A. and Marco-Parra, L. M. (2003). “Direct analysis of blood serum by total reflection X-ray fluorescence spectrometry and application of an artificial neural network approach for cancer diagnosis,” Spectrochim. Acta B 58, 2205–2213.CrossRefGoogle Scholar
Marco, L. M., Greaves, E. D., and Alvarado, J. (1999). “Analysis of human blood serum and human brain samples by total reflection X-ray fluorescence spectrometry applying Compton peak standardization,” Spectrochim. Acta B 54, 1469–1480.Google Scholar
National Heart, Lung and Blood Institute (2013). Body Mass Index Calculator. http://www.nhlbi.nih.gov/guidelines/obesity/BMI/bmicalc.htm. Accessed July 2013.Google Scholar
Nordberg, G. F., Fowler, B. A., Nordberg, M., and Friberg, L. (Eds.) (2007). Handbook on the Toxicology of Metals (Elsevier, Oxford), 3rd ed.Google Scholar
Praamsma, M. L., Arnason, J. G., and Parsons, P. J. (2011). “Monitoring Mn in whole blood and urine: a comparison between electrothermal atomic absorption and inorganic mass spectrometry,” J. Anal. At. Spectrom. 26, 1224–1232.Google Scholar
Seiler, H. G., Sigel, A., and Sigel, H. (Eds.) (1994). Handbook on Metals in Clinical and Analytical Chemistry (Marcel Dekker, NY).Google Scholar
Stosnach, H. and Mages, M. (2009). “Analysis of nutrition-relevant trace elements in human blood and serum by means of total reflection X-ray fluorescence (TXRF) spectroscopy,” Spectrochim. Acta B 64, 354–356.Google Scholar
Varga, I., Szebeni, A., Szoboszlai, N., and Kovacs, B. (2005). “Determination of trace elements in human liver biopsy samples by ICP-MS and TXRF: hepatic steatosis and nickel accumulation,” Anal. Bioanal. Chem. 383, 476–482.Google Scholar
Von Bohlen, A., Klockenkaemper, R., Toelg, G., and Wiecken, B. (1988). “Microtome sections of biomaterials for trace analysis by TXRF,” Fresenius Z. Anal. Chem. 331, 454–458.Google Scholar
World Health Organization, Food and Agriculture Organization of the United Nations, (2002). “Human Mineral and Vitamin Requirements. http://www.fao.org, Accessed September 2013.Google Scholar
Zeimer, R., Weinreb, A., Loewinger, E., Kalman, Z. H., and Belkin, M. (1974). “Detection and analysis of metals in the eye by x-ray spectrometry,” Med. Phys. 1(5), 251–256.Google Scholar