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Elemental mapping in slices of human brain by SR-μXRF

Published online by Cambridge University Press:  01 March 2012

N. Zoeger ,
C. Streli ,
P. Wobrauschek ,
C. Jokubonis ,
G. Pepponi ,
P. Roschger ,
S. Bohic  and
W. Osterode
N. Zoeger*
Affiliation:
Atominstitut, TU-Wien, A-1020 Vienna, Austria
C. Streli
Affiliation:
Atominstitut, TU-Wien, A-1020 Vienna, Austria
P. Wobrauschek
Affiliation:
Atominstitut, TU-Wien, A-1020 Vienna, Austria
C. Jokubonis
Affiliation:
Atominstitut, TU-Wien, A-1020 Vienna, Austria
G. Pepponi
Affiliation:
ITC-irst Centro per la Ricerca Scientifica e Tecnologica, via Sommarive 18, 38050 Povo, Trento, Italy
P. Roschger
Affiliation:
Ludwig Boltzmann Institute of Osteology at the Hanusch Hospital of WGKK and AUVA Trauma Centre Meidling, 4th Medical Department, Hanusch Hospital, Vienna, Austria
S. Bohic
Affiliation:
Micro-FID ID 22, ESRF, BP 220, 38043 Grenoble, France
W. Osterode
Affiliation:
Universitätsklinik für Innere Medizin IV, Vienna, Austria
*
a)Electronic mail: [email protected]
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Abstract

Cognitive deficits are repeatedly described with Pb exposure, but little is known about the distribution of lead in brain. To determine the local distribution of lead (Pb) and other trace elements, X-ray fluorescence spectroscopy measurements have been performed, using a microbeam setup and highest flux synchrotron radiation. Experiments have been carried out at ID-22, ESRF, Grenoble, France. The installed microprobe setup provides a monochromatic beam (16 keV) from an undulator station focused by Kirkpatrick-Baez X-ray optics to a spot size of 5 μm×3 μm. Brain slices from frontal cortex, thalamus, and hippocampus have been investigated (20 μm thickness, imbedded in paraffin and mounted on kapton foils). In general no significant increase in fluorescence intensities could be detected in one of the investigated brain compartments. Pb and other (trace) elements such as S, Ca, Fe, Cu, Zn, and Br could be detected in all samples and showed strong inhomogeneities within the analysed areas. While S, Ca, Fe, Cu, Zn, and Br could be clearly assigned to brain vessel structures (blood vessels, plexus choroidei), Pb was very inhomogeneously distributed. The local distribution of the detected elements in various brain structures will be discussed.

Type
XRF Characterization
Information
Powder Diffraction , Volume 20 , Issue 2 , June 2005 , pp. 158 - 160
Copyright
Copyright © Cambridge University Press 2005

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