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Utilization of M. sativa ssp. caerulea × M. sativa ssp. sativa hybridization in improvement of alfalfa aluminium tolerance

Published online by Cambridge University Press:  29 November 2016

Dragan Milić ,
Ksenija Taški-Ajduković Open the ORCID record for Ksenija Taški-Ajduković [Opens in a new window] ,
Nevena Nagl ,
Jovanka Atlagić  and
Đura Karagić
Dragan Milić
Affiliation:
Institute of Field and Vegetable Crops, M. Gorkog 30, 21000 Novi Sad, Serbia
Ksenija Taški-Ajduković*
Affiliation:
Institute of Field and Vegetable Crops, M. Gorkog 30, 21000 Novi Sad, Serbia
Nevena Nagl
Affiliation:
Institute of Field and Vegetable Crops, M. Gorkog 30, 21000 Novi Sad, Serbia
Jovanka Atlagić
Affiliation:
Institute of Field and Vegetable Crops, M. Gorkog 30, 21000 Novi Sad, Serbia
Đura Karagić
Affiliation:
Institute of Field and Vegetable Crops, M. Gorkog 30, 21000 Novi Sad, Serbia
*
*Corresponding author. E-mail: [email protected]
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Abstract

Sensitivity of alfalfa to acidity and aluminium (Al) toxicity in soil is the major limiting factor in broadening of its growing area. Due to lack of Al tolerance in primary alfalfa germplasm, there is a need for transfer of genes for Al tolerance from other Medicago germplasm. One of the identified sources of Al tolerance is M. sativa ssp. caerulea accession PI 464724, which was used as a female parent in our study. The objectives of this study were: (i) obtaining the tetraploid offspring from 2x–4x M. sativa ssp. caerulea – M. sativa ssp. sativa spontaneous crosses, and (ii) development of a breeding strategy for Al/acid tolerance in alfalfa, using M. sativa ssp. caerulea as a source of Al tolerance. Out of eleven fully developed plants, five were morphologically similar to M. sativa ssp. caerulae, while six plants were similar to M. sativa ssp. sativa. All tested plants were fertile, with the pollen viability ranging from 21.45 to 97.09% and the average number of ovules per plant from 8.80 to 12.29. Eleven SSR primer pairs confirmed the hybrid nature of M. sativa ssp. caerulae × M. sativa ssp. sativa offspring. Both the Cluster Analysis and the Principal Coordinates Analysis separated plants in the caerulae type from plants in the sativa type, with one exception. Strategies based on conventional and molecular marker breeding efforts could lead towards development of tolerant alfalfa cultivars and successful crop production on acidic, Al-contaminated soils.

Keywords

acid tolerancealfalfacrop relativesgenetic resourcesSSR markers
Type
Research Article
Information
Plant Genetic Resources , Volume 16 , Issue 1 , February 2018 , pp. 68 - 73
Copyright
Copyright © NIAB 2016 

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References

Acharya, NS (2014) AAC Meadowview alfalfa. Canadian Journal of Plant Science 94: 187–189.CrossRefGoogle Scholar
Atlagić, J, Terzić, S and Marjanović-Jeromela, A (2012) Staining and fluorescent microscopy methods for pollen viability determination in sunflower and other plant species. Industrial Crops and Products 35: 88–91.CrossRefGoogle Scholar
Barcaccia, G, Tavoletti, S, Mariani, A and Veronesi, F (2003) Occurrence, inheritance and use of reproductive mutants in alfalfa improvement. Euphytica 133: 37–56.CrossRefGoogle Scholar
Bingham, ET and Saunders, JW (1974) Chromosome manipulations in alfalfa: scaling the cultivated tetraploid to seven ploidy levels. Crop Science 14: 474–477.CrossRefGoogle Scholar
Bouton, JH (1996) Screening the alfalfa core collection for acid soil tolerance. Crop Science 36: 198–200.CrossRefGoogle Scholar
Bouton, JH (2012) Breeding lucerne for persistence. Crop Pasture Science 63: 95–106.CrossRefGoogle Scholar
Dall'Agnol, M, Buton, JH and Parrott, WA (1996) Screening methods to develop alfalfa populations tolerant to acid aluminum toxic soils. Crop Science 36: 64–70.CrossRefGoogle Scholar
Dattee, Y (1972) Analyse quantitative de l'auto-et de l'interfertilite chez quelques familles de luzern. Ann Amelior Plant 22, 5–21.Google Scholar
Diwan, N, Bouton, JH, Kochert, G and Cregan, PB (2000) Mapping of simple sequence repeat (SSR) DNA markers in diploid and tetraploid alfalfa. Theoretical and Applied Genetics 101: 165–172.CrossRefGoogle Scholar
Gomes, TF, Borges, CA, Neves, JCL and Fontes, PCR (2002) Nodulation, nitrogen fixation and alfalfa dry matter production as affected by rates of limestone with different calcium: magnesium ratios applied. Ciencia Rural 32: 925–930.CrossRefGoogle Scholar
Grewal, HS and Williams, R (2003) Liming and cultivars affect root growth, nodulation, leaf to stem ratio, herbage yield, and elemental composition of alfalfa on an acid soil. Journal of Plant Nutrition 26: 1683–1696.CrossRefGoogle Scholar
Hair, JF, Black, WC, Babin, BJ, Anderson, RE and Tatham, RL (2006) Multivariate Data Analysis, 6th edn. Upper Saddle River, NJ: Pearson Prentice Hall.Google Scholar
Havananda, T, Brummer, EC and Doyle, JJ (2011) Complex patterns of autopolyploid evolution in alfalfa and allies (Medicago sativa; Leguminosae). American Journal of Botany 98: 1633–1646.CrossRefGoogle ScholarPubMed
Julier, B, Flajoulot, S, Barre, P, Cardinet, G, Santoni, S, Huguet, T and Huyghe, C (2003) Construction of two genetic linkage maps in cultivated tetraploid alfalfa (Medicago sativa) using microsatellite and AFLP markers. BMC Plant Biology 3: 9.CrossRefGoogle ScholarPubMed
Khu, DM, Reyno, R, Han, Y, Zhao, PX, Bouton, JH, Brummer, EC and Monteros, MJ (2013) Identification of aluminum tolerance quantitative trait loci in tetraploid alfalfa. Crop Science 52: 161–167.CrossRefGoogle Scholar
Mohammadi, SA and Prasanna, BM (2003) Analysis of genetic diversity in crop plants-salient statistical tools and considerations. Crop Science 43: 1235–1248.CrossRefGoogle Scholar
Narasimhamoorthy, B, Bouton, JH, Olsen, KM and Sledge, MK (2007) Quantitative trait loci and candidate gene mapping of aluminum tolerance in diploid alfalfa. Theoretical and Applied Genetics 114: 901–913.CrossRefGoogle ScholarPubMed
Nei, M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583–590.CrossRefGoogle ScholarPubMed
Page, RDM (1996) TREEVIEW: an application to display phylogenetic trees on personal computers. Computer Applications in the Biosciences 12: 357–358.Google ScholarPubMed
Peakall, R and Smouse, PE (2006) GenAlEx 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6: 288–295.CrossRefGoogle Scholar
Quiros, CF and Bauchan, GR (1988) The genus Medicago and the origin of the Medicago sativa complex. In: Hanson, AA, Barnes, DK and Hill, RR (ed.) Alfalfa and Alfalfa Improvement. Madison: ASA–CSSA–SSSA, pp. 93–124.Google Scholar
Reyno, R, Khu, DM, Monteros, MJ, Bouton, JH, Parrot, W and Brummer, EC (2013) Evaluation of two transgenes for aluminum tolerance in alfalfa. Crop Science 53: 1581–1588.CrossRefGoogle Scholar
Şakiroğlu, M and Brummer, EC (2011) Clarifying the ploidy of some accessions in the USDA alfalfa germplasm collection. Turkish Journal of Botany 35: 509–519.Google Scholar
Sledge, MK, Bouton, JH, Dall'Agnoll, M, Parrott, WA and Kochert, G (2002) Identification and confirmation of aluminum tolerance QTL in diploid Medicago sativa subsp. caerulea . Crop Science 42: 1121–1128.CrossRefGoogle Scholar
Sledge, MK, Pechter, P and Payton, ME (2005) Aluminum tolerance in Medicago truncatula germplasm. Crop Science 45: 2001–2004.CrossRefGoogle Scholar
Somma, M (2004) Extraction and purification of DNA. In: Querci, M, Jermini, M and Van den Eade, G (ed.) The Analysis of Food Samples for the Presence of Genetically Modified Organisms. Ispra: European Commision, Joint Research Centre, Ch. 4.Google Scholar
Veronesi, F, Mariani, A and Bingham, ET (1986) Unreduced gametes in diploid Medicago and their importance in alfalfa breeding. Theoretical and Applied Genetics 72: 37–41.CrossRefGoogle ScholarPubMed
Yeh, FC, Yang, RC and Boyle, T (1997) POPGENE, the User Friendly Software for Population Genetic Analysis. Molecular Biology and Biotechnology Center, University of Alberta, Edmonton, Alberta, Canada.Google Scholar
Zhang, XG, Humphries, A and Auricht, G (2007) Genetic variability and inheritance of aluminium tolerance as indicated by long root regrowth in lucerne (Medicago sativa L.). Euphytica 157: 177–184.CrossRefGoogle Scholar