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Transfer of simple sequence repeat (SSR) markers across the legume family for germplasm characterization and evaluation

Published online by Cambridge University Press:  16 October 2024

M. L. Wang ,
A. G. Gillaspie ,
M. L. Newman ,
R. E. Dean ,
R. N. Pittman ,
J. B. Morris  and
G. A. Pederson
M. L. Wang*
Affiliation:
USDA-ARS, Plant Genetic Resources Conservation Unit, 1109 Experiment Street, Griffin, GA 30223, USA
A. G. Gillaspie
Affiliation:
USDA-ARS, Plant Genetic Resources Conservation Unit, 1109 Experiment Street, Griffin, GA 30223, USA
M. L. Newman
Affiliation:
USDA-ARS, Plant Genetic Resources Conservation Unit, 1109 Experiment Street, Griffin, GA 30223, USA
R. E. Dean
Affiliation:
USDA-ARS, Plant Genetic Resources Conservation Unit, 1109 Experiment Street, Griffin, GA 30223, USA
R. N. Pittman
Affiliation:
USDA-ARS, Plant Genetic Resources Conservation Unit, 1109 Experiment Street, Griffin, GA 30223, USA
J. B. Morris
Affiliation:
USDA-ARS, Plant Genetic Resources Conservation Unit, 1109 Experiment Street, Griffin, GA 30223, USA
G. A. Pederson
Affiliation:
USDA-ARS, Plant Genetic Resources Conservation Unit, 1109 Experiment Street, Griffin, GA 30223, USA
*
* Corresponding author. E-mail: [email protected]
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Abstract

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A set of 68 simple sequence repeat (SSR) markers were selected from existing databases (including Medicago, soybean, cowpea and peanut) for the purpose of exploiting the transferability of SSRs across species and/or genera within the legume family. Primers were tested for cross-species and cross-genus fragment amplification with an array of 24 different legume accessions. Nearly one-third (30.78%) of the SSR primers screened generated reproducible and cross-genus amplicons. One hundred and seventeen cross-species polymorphic amplicons were identified and could be used as DNA markers. These polymorphic markers are now being used for characterization and evaluation of our collected and donated legume germ- plasm. The transferability of SSRs, mis-/multiple-primings, homologous/heterologous amplifications, single/multiple-amplicons and application of these amplicons as DNA markers are discussed. The transfer of SSR markers across species or across genera can be a very efficient approach for DNA marker development, especially for minor crops.

Keywords

characterization and evaluationgermplasmlegumeSSRtransfer
Type
Research Article
Information
Plant Genetic Resources , Volume 2 , Issue 2 , August 2004 , pp. 107 - 119
Copyright
© NIAB 2004

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