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Sequence-related amplified polymorphism and inter-simple sequence repeat marker-based genetic diversity and nuclear DNA content variation in common vetch (Vicia sativa L.)

Published online by Cambridge University Press:  15 July 2015

Abdullah Cil  and
Iskender Tiryaki
Abdullah Cil
Affiliation:
East Mediterranean Agriculture Research Institute, Yuregir, Adana, Turkey
Iskender Tiryaki*
Affiliation:
Department of Agricultural Biotechnology, Faculty of Agriculture, Canakkale Onsekiz Mart University, Canakkale17100, Turkey
*
*Corresponding author. E-mail: [email protected]
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Abstract

Genetic diversity of 30 common vetch (Vicia sativa L.) lines and cultivars obtained from various resources or collected from natural flora of Turkey was evaluated by using 55 sequence-related amplified polymorphism (SRAP) and five inter-simple sequence repeat (ISSR) primer sets, and their nuclear DNA contents were determined by flow cytometer. A total of 188 polymorphic loci were detected, with an average of 3.62 loci per primer. The percentage of polymorphic loci was 82.1%. The polymorphism information content values ranged from 0.12 to 0.96, with an average of 0.63. The genetic distance coefficients were in the range of 0.112–0.627. Cluster analysis revealed that the 27 lines and three cultivars could be divided into two main groups. No polyploidy was detected within any vetch lines tested while significant (P< 0.0001) nuclear DNA content differences were determined. The present study revealed that fast and accurate fingerprinting analysis could be done using SRAP and ISSR markers, which indicated existing significant variation among common vetch lines and cultivars.

Keywords

DNA contentgenetic relationshipgenome sizeinter-simple sequence repeatpolymorphism information contentsequence-related amplified polymorphismVicia sativa
Type
Research Article
Information
Plant Genetic Resources , Volume 14 , Issue 3 , September 2016 , pp. 183 - 191
Copyright
Copyright © NIAB 2015 

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