Hostname: page-component-cd9895bd7-dzt6s Total loading time: 0 Render date: 2024-12-26T18:05:51.114Z Has data issue: false hasContentIssue false
  • English
  • Français

Screening of cultivated and wild Helianthus species reveals herbicide tolerance in wild sunflowers and allelic variation at Ahasl1 (acetohydroxyacid synthase 1 large subunit) locus

Published online by Cambridge University Press:  05 April 2016

Jinu Jacob ,
Mulpuri Sujatha  and
Sivannarayana Kodeboyina Varaprasad
Jinu Jacob*
Affiliation:
ICAR-Indian Institute of Millets Research, Hyderabad, Telangana State, 500030, India
Mulpuri Sujatha
Affiliation:
ICAR-Indian Institute of Oilseeds Research, Hyderabad, Telangana State, 500030, India
Sivannarayana Kodeboyina Varaprasad
Affiliation:
ICAR-Indian Institute of Oilseeds Research, Hyderabad, Telangana State, 500030, India
*
*Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Acetohydroxyacid synthase (AHAS) inhibiting herbicides have played a significant role in effective weed control in the cultivation of sunflower since their discovery. The development of sunflower lines and hybrids resistant to these herbicides made their post-emergence application possible. In this study, wild Helianthus species were screened phenotypically for imidazolinone (IMI) and sulfonylurea (SU) resistance by herbicide spray and for allelic variations at Ahasl1 (Ahas1 large subunit) locus using simple sequence repeat and single nucleotide polymorphism markers. Helianthus praecox accession 1823 plants showed promising resistance to three classes of SU-based herbicides and Helianthus nuttallii accession NUT05 plants remained green for a longer time after imazethapyr spray. A total of 50 accessions belonging to 21 wild Helianthus species of different ploidy levels were analysed for allelic variation along with some parents of commercial sunflower hybrids. None of the wild species showed the resistant allele (Ahasl1-1) similar to SCG101 (IMI-resistant line), although it is present in some of the parental lines of hybrids. However, the parental lines having Ahasl1-1-type allele failed to survive the field dose of imazethapyr spray. Inter-species and inter-accessional allelic variation could be observed among the species. PRA1823 and NUT05 showed repeat length variations at Ahasl1 locus. Sequencing of full length Ahasl1 gene from both these accessions did not reveal any resistance mutations in the protein sequences. The molecular basis of the phenotypes identified in this study could be explored further and utilized in breeding programmes for imparting herbicide resistance in cultivated hybrids across sunflower growing regions of the world.

Keywords

imazethapyrSSR markersulfonylureasunflower
Type
Research Article
Information
Plant Genetic Resources , Volume 15 , Issue 5 , October 2017 , pp. 421 - 429
Copyright
Copyright © NIAB 2016 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Al-Khatib, K, Baumgartner, JR, Peterson, DE and Currie, RS (1998) Imazethapyr resistance in common sunflower (Helianthus annuus). Weed Science 46: 403–407.CrossRefGoogle Scholar
Al-Khatib, K, Baumgartner, JR and Currie, RS (1999) Survey of common sunflower (Helianthus annuus) resistance to ALS-inhibiting herbicides in northeast Kansas. In: Proceedings of 21th Sunflower Research Workshop, USA, pp. 210–215.Google Scholar
Block, C (1992) Screening for Alternaria head blight resistance in the USDA germplasm collection. In: Proceedings of 14th Sunflower Research Workshop. Bismarck, ND, USA: National Sunflower Association, pp. 64–66.Google Scholar
Breccia, G, Gil, M, Vega, T, Zorzoli, R, Picardi, L and Nestares, G (2012) Effect of cytochrome P450s inhibitors on imidazolinone resistance in sunflower. In: Proceedings of 18th Sunflower Conference, Mar del Plata-Balcarce, Argentina, pp. 507–512.Google Scholar
Brown, HM (1990) Mode of action, crop selectivity, and soil relations of the sulfonylurea herbicides. Pesticide Science 29: 263–281.CrossRefGoogle Scholar
Bruniard, JM and Miller, JF (2001) Inheritance of imidazolinone-herbicide resistance in sunflower. Helia 24: 11–16.CrossRefGoogle Scholar
Bulos, M, Sala, CA, Altieri, E and Ramos, ML (2013) Marker assisted selection for herbicide resistance in sunflower. Helia 36: 1–16.CrossRefGoogle Scholar
Cantamutto, M, Poverene, M, Presotto, A, Alvarez, D, Lenardon, S, Rodriguez, R, Martin, SJ, Fernandez, MI, Giolitti, F, Garayalde, A, Haucke, A, Bellido, A and Fraysse, M (2010) The Argentine wild Helianthus annuus L. genetic resource. Helia 33: 47–62.CrossRefGoogle Scholar
D'Halluin, KM, Bossut, M, Bonne, E, Mazur, B, Leemans, J and Botterman, J (1992) Transformation of sugarbeet (Beta vulgaris L.) and evaluation of herbicide resistance in transgenic plants. Nature Biotechnology 10: 309–314.CrossRefGoogle Scholar
Doyle, JJ and Doyle, JL (1990) Isolation of plant DNA from fresh tissue. Focus 12: 13–15.Google Scholar
FAO Statistics (2013) Sunflower crude and refined oils. In: Agribusiness Handbook. Rome, Italy: Food and Agriculture Organization (FAO), p. 9.Google Scholar
Hawley, RM (2005) The acetohydroxyacid gene family: its role in herbicide resistant sunflowers. M.Sc. Thesis, Oregon State University, Corvallis.Google Scholar
Jan, CC and Chandler, JM (1985) Transfer of powdery mildew resistance from Helianthus debilis Nutt. to cultivated sunflower (H. annuus L.). Crop Science 25: 664–666.CrossRefGoogle Scholar
Kolkman, JM, Slabaugh, MB, Bruniard, JM, Berry, S, Bushman, BS, Olungu, C, Maes, N, Abratti, G, Zambelli, A and Miller, JF (2004) Acetohydroxyacid synthase mutations conferring resistance to imidazolinone or sulfonylurea herbicides in sunflower. Theoretical and Applied Genetics 109: 1147–1159.CrossRefGoogle ScholarPubMed
Liu, Z, Wang, D, Feng, J, Seiler, GJ, Cai, X and Jan, CC (2013) Diversifying sunflower germplasm by integration and mapping of a novel male fertility restoration gene. Genetics 193: 727–737.CrossRefGoogle ScholarPubMed
McCourt, JA, Pang, SS, King-Scott, J, Guddat, LW and Duggleby, RG (2006) Herbicide-binding sites revealed in the structure of plant acetohydroxyacid synthase. Proceedings of National Academy of Sciences of the United States of America 103: 569–573.CrossRefGoogle ScholarPubMed
Miller, JF and Al-Khatib, K (2002) Imidazolinone herbicide-resistant sunflower germplasms. Crop Science 42: 988.CrossRefGoogle Scholar
Miller, JF and Al-Khatib, K (2004) Registration of two oilseed sunflower genetic stocks, SURES-1 and SURES-2, resistant to tribenuron herbicide. Crop Science 44: 1037–1038.CrossRefGoogle Scholar
Miller, JF and Gulya, TJ (1988) Registration of six downy mildew resistant sunflower germplasm lines. Crop Science 28: 1040–1041.CrossRefGoogle Scholar
Miller, JF and Gulya, TJ (1991) Inheritance of resistance to race 4 of downy mildew derived from interspecific crosses in sunflower. Crop Science 31: 40–43.CrossRefGoogle Scholar
Miller, JF and Seiler, GJ (2005) Tribenuron resistance in accessions of wild sunflower collected in Canada. In: Proceedings of the 27th Sunflower Research Workshop. Bismarck, ND: National Sunflower Association. Available at http://www.sunflowernsa.com/uploads/research/185/Miller_Tribenuron_05.PDF.Google Scholar
Prathap Reddy, K, Chander Rao, S, Kirti, PB and Sujatha, M (2013) Development of a scoring scale for powdery mildew (Golovinomyces cichoracearum (DC.) V.P. Heluta) disease and identification of resistance sources in cultivated and wild sunflowers. Euphytica 190: 385–399.CrossRefGoogle Scholar
Presotto, A, Ureta, MS, Cantamutto, M and Poverene, M (2012) Effects of gene flow from IMI resistant sunflower crop to wild Helianthus annuus populations. Agriculture, Ecosystems and Environment 146: 153–161.CrossRefGoogle Scholar
Rajasekaran, K, Grula, JW and Anderson, DM (1996) Selection and characterization of mutant cotton (Gossypium hirsutum L.) cell lines resistant to sulfonylurea and imidazolinone herbicides. Plant Science 199: 115–124.CrossRefGoogle Scholar
Rogers, CE and Thompson, TE (1978) Resistance in wild Helianthus to the sunflower beetle. Journal of Economic Entomology 71: 622–623.CrossRefGoogle Scholar
Rogers, CE and Thompson, TE (1980) Helianthus resistance to the sunflower beetle. Journal of the Kansas Entomological Society 53: 727–730.Google Scholar
Saari, LL, Cotterman, JC and Thill, DC (1994) Resistance to acetolactate synthase inhibiting herbicides. In: Powles, SB and Holtum, JAM (eds) Herbicide Resistance in Plants: Biology and Biochemistry. Boca Raton: Lewis Publishers, pp. 83–139.Google Scholar
Sala, CA and Bulos, M (2012) Inheritance and molecular characterization of broad range tolerance to herbicides targeting acetohydroxyacid synthase in sunflower. Theoretical and Applied Genetics 124: 355–364.CrossRefGoogle ScholarPubMed
Sala, CA, Bulos, M, Echarte, AM, Whitt, S, Budziszewski, G, Howie, W, Singh, B and Weston, B (2008a) Development of CLHA-Plus: a novel herbicide tolerance trait in sunflower conferring superior imidazolinone tolerance and ease of breeding. In: Proceedings of 17th International Sunflower Conference, Córdoba, Spain, pp. 489–494.Google Scholar
Sala, CA, Bulos, M and Echarte, AM (2008b) Genetic analysis of an induced mutation conferring imidazolinone resistance in sunflower. Crop Science 48: 1817–1822.CrossRefGoogle Scholar
Sala, CA, Bulos, M, Altieri, E and Ramos, ML (2012) Genetics and breeding of herbicide tolerance in sunflower. Helia 35: 57–70.Google Scholar
Saliman, M, Yang, SM and Wilson, L (1982) Reaction of Helianthus species to Erysiphe cichoracearum . Plant Disease Journal 66: 572–573.CrossRefGoogle Scholar
Schilling, EE and Heiser, CB Jr (1981) Infrageneric classification of Helianthus (Compositae). Taxon 30: 393–403.CrossRefGoogle Scholar
Sebastian, SA, Fader, GM, Ulrich, JF, Forney, DR and Chaleff, RS (1989) Semi-dominant soybean mutation for resistance to sulfonylurea herbicides. Crop Science 29: 1403–1408.CrossRefGoogle Scholar
Seiler, GJ (1992) Utilization of wild sunflower species for the improvement of cultivated sunflower. Field Crops Research 30: 195–230.CrossRefGoogle Scholar
Seiler, GJ (2007) Wild annual Helianthus anomalus and H. deserticola for improving oil content and quality in sunflower. Industrial Crops and Products 25: 95–100.CrossRefGoogle Scholar
Seiler, GJ, Christie, BR and Choo, TM (1991) Registration of 13 downy mildew tolerant interspecific sunflower germplasm lines derived from wild annual species. Crop Science 31: 1714–1716.Google Scholar
Sujatha, M (2006) Wild Helianthus species used for broadening the genetic base of cultivated sunflower in India. Helia 29: 77–86.CrossRefGoogle Scholar
Terzic, S and Atlagic, J (2008) Tribenuron-methyl resistance in accessions of annual wild sunflower species from the NoviSad germplasm collection. In: Proceedings of 17th International Sunflower Conference, June 8–12, 2008, Cordoba, Spain, pp. 667–671.Google Scholar
Tikhomirov, VT and Chiryaev, PV (2005) Sources of resistance to diseases in original material of sunflower. Helia 28: 101–106.CrossRefGoogle Scholar
Tranel, PJ and Wright, TR (2002) Resistance to ALS-inhibiting herbicides: what have we learned? Weed Science 50: 700–712.CrossRefGoogle Scholar
Vranceanu, VL, Pirvu, N and Stoenescu, FM (1981) New sunflower downy mildew resistance genes and their management. Helia 4: 23–27.Google Scholar
Supplementary material: File

Jacob et al supplementary material

Figure S1

Download Jacob et al supplementary material(File)
File 81.8 KB