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Rationalization of taro germplasm collections in the Pacific Island region using simple sequence repeat (SSR) markers

Published online by Cambridge University Press:  08 March 2007

E. S. Mace ,
P. N. Mathur ,
L. Izquierdo ,
D. Hunter ,
M. B. Taylor ,
D. Singh ,
I. H. DeLacy ,
G. V. H. Jackson  and
I. D. Godwin
E. S. Mace*
Affiliation:
School of Land and Food Sciences, The University of Queensland, Brisbane, Qld 4072, Australia
P. N. Mathur
Affiliation:
IPGRI Office for South Asia, c/o NBPGR, Pusa Campus, New Delhi, 110 012, India
L. Izquierdo
Affiliation:
School of Land and Food Sciences, The University of Queensland, Brisbane, Qld 4072, Australia
D. Hunter
Affiliation:
SPC (Secretariat of the Pacific Community), Suva, Fiji
M. B. Taylor
Affiliation:
SPC (Secretariat of the Pacific Community), Suva, Fiji
D. Singh
Affiliation:
National Agricultural Research Institute, Lae, Papua New Guinea, Sydney, NSW 2002, Australia
I. H. DeLacy
Affiliation:
School of Land and Food Sciences, The University of Queensland, Brisbane, Qld 4072, Australia
G. V. H. Jackson
Affiliation:
24 Att Street, Queens Park, Sydney, NSW 2002, Australia
I. D. Godwin
Affiliation:
School of Land and Food Sciences, The University of Queensland, Brisbane, Qld 4072, Australia
*
*Corresponding author (present address): Hermitage Research Station, 604 Yangan Road, Warwick, Qld 4370, Australia. E-mail: [email protected]
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Abstract

A regional (Oceania) core collection for taro germplasm has been developed based on phenotypic and molecular characterization. In total, 2199 accessions of taro germplasm have been collected by TaroGen (Taro Genetic Resources: Conservation and Utilisation) from 10 countries in Oceania: Papua New Guinea, Solomon Islands, Vanuatu, New Caledonia, Fiji, Palau, Niue, Tonga, Cook Islands and Samoa. Our objective was to select 10% from each country to contribute to a regional core. The larger collections from Papua New Guinea, Vanuatu and New Caledonia were analysed based on phenotypic characters, and a diverse subset representing 20% of these collections was fingerprinted. A diverse 20% subsample was also taken from the Solomon Islands. All accessions from the other six countries were fingerprinted. In total, 515 accessions were genotyped (23.4% overall) using taro specific simple sequence repeat (SSR) markers. DNA fingerprint data showed that great allelic diversity existed in Papua New Guinea and the Solomon Islands. Interestingly, rare alleles were identified in taros from the Solomon Islands province of Choiseul which were not observed in any of the other collections. Overall, 211 accessions were recommended for inclusion in the final regional core collection based on the phenotypic and molecular characterization.

Keywords

genetic diversityPacific Island regionSSRstaro
Type
Research Article
Information
Plant Genetic Resources , Volume 4 , Issue 3 , December 2006 , pp. 210 - 220
Copyright
Copyright © NIAB 2006

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References

Brown, AHD and Spillane, C (1999) Implementing core collections–principles, procedures, progress, problems and promise. In: Johnson, RC and Hodgkin, T(eds) Core Collections for Today and Tomorrow. Rome: International Plant Genetic Resources Institute, pp. 1–9.Google Scholar
Coates, DJ, Yen, DE and Gaffey, PM (1988) Chromosome variation in taro. Colocasia esculenta: implications for its origin in the Pacific. Cytologia 53: 551–560.CrossRefGoogle Scholar
de Vicente, C, Metz, T and Alercia, A (2004) Descriptors for Genetic Marker Technologies. Rome: International Plant Genetic Resources Institute.Google Scholar
Dice, LR (1945) Measures of the amount of ecologic association between species. Ecology 26: 297–302.CrossRefGoogle Scholar
Excoffier, L, Smouse, PE and Quattro, JM (1992) Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131: 479–491.CrossRefGoogle ScholarPubMed
FAOSTAT (2005) FAO Statistical Database: Agricultural Production of Primary Crops, http://apps.fao.org/default.htm.Google Scholar
Ford-Lloyd, BV (2001) Genotyping in plant genetic resources. In: Henry, RJ(ed.) Plant Genotyping: The DNA Fingerprinting of Plants. Cambridge: CAB International, pp. 59–82.CrossRefGoogle Scholar
Godwin, ID, Mace, ES and Nurzuhairawaity, (2001) Genotyping Pacific Island taro ( Colocasia esculenta (L.) Schott) germplasm. In: Henry, RJ(ed.) Plant Genotyping: The DNA Fingerprinting of Plants. Cambridge: CAB International, pp. 109–128.CrossRefGoogle Scholar
Hokanson, SC, Szewc-McFadden, AK, Lamboy, WF and McFerson, JR (1998) Microsatellite (SSR) markers reveal genetic identities, genetic diversity and relationships in a Malus × domestica Borkh. core subset collection. Theoretical and Applied Genetics 97: 671–683.CrossRefGoogle Scholar
Irwin, SV, Kaufusi, P, Banks, K, de la Peña, R and Cho, JJ (1998) Molecular characterisation of taro ( Colocasia esculenta ) using RAPD markers. Euphytica 99: 183–189.CrossRefGoogle Scholar
Jaccard, P (1908) Nouvelles recherches sur la distribution florale. Bulletin de la Société Vaudoise des Sciences Naturelles 44: 223–270.Google Scholar
Jackson, GVH and Firman, ID (1987) Characterization and preliminary evaluation of root crop germplasm in the South Pacific with special reference to pests and diseases. IBPGR/RECSEA Newsletter, Special Edition, pp. 19–23.Google Scholar
Kimura, M and Crow, JF (1964) The number of alleles that can be maintained in a finite population. Genetics 49: 725–738.CrossRefGoogle Scholar
Kreike, CM, van Eck, HJ and Lebot, V (2004) Genetic diversity of taro, Colocasia esculenta (L.) Schott, in Southeast Asia and the Pacific. Theoretical and Applied Genetics 109: 761–768.CrossRefGoogle ScholarPubMed
Kuruvilla, KM and Singh, A (1981) Karyotypic and electrophoretic studies on taro and its origins. Euphytica 30: 405–412.CrossRefGoogle Scholar
Lebot, V (1992) Genetic vulnerability of Oceania's traditional crops. Experimental Agriculture 29: 309–323.CrossRefGoogle Scholar
Lebot, V and Aradhya, KM (1991) Isozyme variation in taro ( Colocasia esculenta (L.) Schott.) in Asia and Oceania. Euphytica 56: 55–66.CrossRefGoogle Scholar
Lebot, V, Prana, MS, Kreike, Nvan Eck, H, Pardales, J, Okpul, T, Gendue, T, Thongkiem, M, Hue, H, Viet, N and Yap, TC (2004) Characterisation of taro ( Colocasia esculenta (L.) Schott) genetic resources in Southeast Asia and Oceania. Genetic Resources and Crop Evolution 51: 381–392.CrossRefGoogle Scholar
Lebot, V, Ivancic, A and Abraham, K (2005) The geographical distribution of allelic diversity, a practical means of preserving and using minor root crop genetic resources. Experimental Agriculture 41: 475–489.CrossRefGoogle Scholar
Léon, J (1977) Origin, evolution, and early dispersal of root and tuber crops. In: Cook, J, MacIntyre, R, Graham, M(eds) Proceedings of the 4th Symposium of the International Society for Tropical Root Crops. Ottawa: International Development Research Centre, pp. 20–36.Google Scholar
Mace, ES and Godwin, ID (2002) Development and characterisation of polymorphic microsatellite markers in taro, Colocasia esculenta (L.) Schott. Genome 45: 823–832.CrossRefGoogle Scholar
Mace, ES, Mathur, PN, Godwin, ID, Hunter, D, Taylor, MB, Singh, D, DeLacy, IH and Jackson, GVH (2006) Development of a Regional Core Collection (Oceania) for taro, Colocasia esculenta (L.) Schott., based on molecular and phenotypic characterization. In: Ramanatha, Rao VPJ, Matthews, PJ and Eyzaguirre, PB(eds) The Global Diversity of Taro: Ethnobotany and Conservation. Rome: IPGRI; Osaka: National Museum of Ethnology (in press).Google Scholar
Matthews, PJ (1990) The origins, dispersal and domestication of taro. PhD Thesis, Australian National University.Google Scholar
Matthews, PJ (1991) A possible tropical wildtype taro: Colocasia esculenta var. aquatilis. Indo-Pacific Prehistory Bulletin 11: 69–81.Google Scholar
Matthews, PJ (1995) Aroids and the Austronesians. Tropics 4: 105–126.CrossRefGoogle Scholar
Morales, EAV, Valois, ACC and Costa, IRS (1995) Core collections for genebanks with limited resources. In: Hodgkin, T, Brown, AHD, van Hintum, TJL and Morales, EAV(eds) Core Collections of Plant Genetic Resources New York: John Wiley and Sons, 241–249.Google Scholar
Nei, M (1987) Molecular Evolutionary Genetics. New York: Colombia University Press.CrossRefGoogle Scholar
Nei, M and Li, W-H (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences USA 76: 5269–5273.CrossRefGoogle ScholarPubMed
Plucknett, DL (1976) Edible aroids: Alocasia, Colocasia, Cyrtosperma, Xanthosoma. In: Simmonds, NW(ed.) Evolution of Crop Plants. London: Longman, pp. 10–12.Google Scholar
Plucknett, DL, de la Peña, RS and Obrero, F (1970) Taro (Colocasia esculenta). Field Crop Abstracts 23: 413–426.Google Scholar
Powell, W, Morgante, M, Andre, C, Hanafey, M, Vogel, J, Tingey, S and Rafalski, A (1996) The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding 2: 225–238.CrossRefGoogle Scholar
Purseglove, JK (1972) Tropical Crops. Monocotyledons I. London: Longman.Google Scholar
Rohlf, FJ (1999) NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System, version 2.02i. New York: Exeter Software.Google Scholar
Savage, Dangl G, Mendum, ML, Prins, BH, Walker, MA, Meredith, CP and Simon, CJ (2001) Simple sequence repeat analysis of a clonally propagated species: a tool for managing a grape germplasm collection. Genome 44: 432–438.Google Scholar
Schneider, S, Roessli, D and Excofier, L (2000) Arlequin: A Software for Population Genetics Data Analysis, version 2.000 Geneva: Genetics and Biometry Lab, Department of Anthropology, University of Geneva.Google Scholar
Sokal, RR and Michener, CD (1958) A statistical method for evaluating systematic relationships. University of Kansas Scientific Bulletin 38: 1409–1438.Google Scholar
Tanimoto, T and Matsumoto, T (1986) Variations of morphological characters and isozyme patterns in Japanese cultivars of Colocasia esculenta Schott and C. gigantea Hook. Japanese Journal of Breeding 36: 100–111.Google Scholar
Teulat, B, Aldam, C, Trehin, R, Lebrun, P, Barker, JHA, Arnold, GM, Karp, A, Baudouin, L and Rognon, F (2000) An analysis of genetic diversity in coconut ( Cocos nucifera ) populations from across the geographic range using sequence-tagged microsatellites (Microsatellites) and AFLPs. Theoretical and Applied Genetics 100: 764–771.CrossRefGoogle Scholar
van Treuren, R, van Soest, LJM and van Hintum, TJL (2001) Marker-assisted rationalisation of genetic resources collections: a case study in flax using AFLPs. Theoretical and Applied Genetics 103: 144–152.CrossRefGoogle Scholar
Yen, DE (1991a) Domestication: the lessons from New Guinea.In: Pawley, A(ed.) Man and a Half. Auckland: Polynesian Society, pp. 558–569.Google Scholar
Yen, DE (1991b) Polynesian cultigens and cultivars: the questions of origin. In: Cox, PA, Banack, SA(eds) Islands, Plants and Polynesia. Portland: Dioscorides Press, pp. 67–95.Google Scholar
Yen, DE (1993) The origins of subsistence agriculture in Oceania and the potential for future tropical food crops. Economic Botany 47: 3–14.CrossRefGoogle Scholar
Yen, DE and Wheeler, JM (1968) Induction of taro into the Pacific: the indications of chromosome numbers. Ethnology 7: 259–267.CrossRefGoogle Scholar