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Population and multilocus isozyme structures in a barley landrace

Published online by Cambridge University Press:  12 February 2007

A.A. Jaradat*
Affiliation:
USDA-ARS, 803 Iowa Avenue, Morris, MN 56267, USA
M. Shahid
Affiliation:
Plant Genetic Resources Program, International Center for Biosaline Agriculture (ICBA), P.O. Box 14660, Dubai, United Arab Emirates
*
*Corresponding author: E-mail: [email protected]

Abstract

Isozyme data were used to assess genetic diversity within and among a subdivided population of the salt-tolerant Batini barley landrace. Population diversity and its components were estimated on the basis of 12 isozymes scored on 450 single plants representing seven subpopulations. Two principal components, based on mean gene diversity, Shannon's diversity index, percentage polymorphic loci, genetic identity and genetic distance among subpopulations accounted for 91.7% of total variation and separated the subpopulations into four distinct groups. Contributions to total diversity by individual subpopulations were partitioned into intra- and inter-population components. The level of population differentiation indicates that a large percentage of total genetic diversity was apportioned within subpopulations. The presence of valuable genetic diversity in this landrace was confirmed.

Type
Research Article
Copyright
Copyright © USDA 2006

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References

Agresti, A (1990) Categorical Data Analysis. New York: John Wiley & Sons.Google Scholar
Berg, EE and Hamrick, JL (1997) Quantification of genetic diversity at allozyme loci. Canadian Journal of Forest Research 27, 415424.CrossRefGoogle Scholar
Bernardo, A, Luque, A, Cuadrado, A, Negro, A, Jouve, N and Soler, C (1997) The assessment of genetic variation in Spanish primitive cultivars of barley Hordeum vulgare L., by a combination of isozymes and hordeins. Genetic Resources and Crop Evolution 44, 217226.CrossRefGoogle Scholar
Brown, ADH (1983) Barley. In: Tanksley, SD and Orton, TJ (eds) Isozymes in Genetics and Breeding, Part I.B. Amsterdam: Elsevier, pp. 5777.CrossRefGoogle Scholar
Brown, ADH and Feldman, MW (1981) Population structure of multilocus associations. Proceedings of the National Academy of Science USA 78, 59135916.CrossRefGoogle ScholarPubMed
Brown, ADH and Marshall, DR (1995) A basic sampling strategy: theory and practice. In: Guarino, L, Rao, R, Reid, R (eds) Collecting Plant Genetic Diversity Technical Guidelines. Wallingford: CAB International, pp. 7591.Google Scholar
Brush, SB (1994) In situ conservation of landraces in centers of diversity. Crop Science 35, 346354.CrossRefGoogle Scholar
Dai, X and Zhang, Q (1989) Genetic diversity of six isozyme loci in cultivated barley of Tibet. Theoretical and Applied Genetics 78, 281286.CrossRefGoogle ScholarPubMed
Demissie, A, Bjørnstad, A (1997) Geographical, altitude and agro-ecological differentiation of isozyme and hordein genotypes of landrace barley from Ethiopia: implications to germplasm conservation. Genetic Resources and Crop Evolution 44, 4355.CrossRefGoogle Scholar
Doebley, J (1989) Isozyme evidence and evolution of crop plants. In: Soltis, ED, Soltis, PM (eds) Isozymes in Plant Biology, Portland, OR: Oregon Dioscorides, pp. 165191.CrossRefGoogle Scholar
Finkeldey, R and Murillo, O (1999) Contribution of subpopulations to total gene diversity. Theoretical and Applied Genetics 98, 664668.CrossRefGoogle Scholar
Hartl, DL and Clark, AG (1989) Principles of Population Genetics, 2nd edn. Sunderland, MA: Sinauer Associates.Google Scholar
Jana, S and Pietrzak, L (1988) Comparative assessment of genetic diversity in wild and primitive cultivated barley in a center of diversity. Genetics 119, 981990.CrossRefGoogle Scholar
Jaradat, AA, Shahid, M, Al-Maskri, AY (2004 a) Genetic diversity in the Batini barley landrace from Oman: I. Spike and seed quantitative and qualitative traits. Crop Science 44, 304315.CrossRefGoogle Scholar
Jaradat, AA, Shahid, M, Al-Maskri, AY (2004 b) Genetic diversity in the Batini barley landrace from Oman: II. Response to salinity stress. Crop Science 44, 9971007.Google Scholar
Labate, J (2000) Software for population genetic analyses of molecular data. Crop Science 40, 15211528.CrossRefGoogle Scholar
Li, Z and Rutger, JN (2000) Geographic distribution and multilocus organization of isozyme variation of rice ( Oryza sativa L.). Theoretical and Applied Genetics 101, 379387.CrossRefGoogle Scholar
Linde-Laursen, I, Nielsen, G and Johansen, HB (1987) Distribution of enzyme markers at 37 loci in a pedigree of European spring barley. Hereditas 106, 241251.CrossRefGoogle Scholar
Liu, F, von Bothmer, R and Salomon, B (1999) Genetic diversity among East Asian accessions of the barley core collection. Theoretical and Applied Genetics 98, 12261233.CrossRefGoogle Scholar
Liu, F, von Bothmer, R and Salomon, B (2000) Genetic diversity in European accessions of the barley core collection as detected by isozyme electrophoresis. Genetic Resources and Crop Evolution 47, 571581.CrossRefGoogle Scholar
Marshall, DR and Brown, AHD (1975) Optimum sampling strategies in genetic conservation. In: Frankel, OH, Hawks, JG (eds) Crop Genetic Resources for Today and Tomorrow, Cambridge, Cambridge University Press, 5370.Google Scholar
Mohammadi, SA and Parsana, BM (2003) Analysis of genetic diversity in crop plants—salient statistical tools and considerations. Crop Science 43, 12351248.CrossRefGoogle Scholar
Nei, M (1973) Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Science USA 70, 33213323.CrossRefGoogle ScholarPubMed
Nei, M (1987) Molecular Evolutionary Genetics. New York: Columbia University Press.CrossRefGoogle Scholar
Nevo, E (2001) Evolution of genome-phenome diversity under environmental stress. Proceedings of the National Academy of Science USA 98, 62336240.CrossRefGoogle ScholarPubMed
Parzies, HK, Spoor, W and Ennos, RA (2004) Inferring seed exchange between farmers from population genetic structure of barley landrace Arabi Aswad from Northern Syria. Genetic Resources and Crop Evolution 51, 471478.CrossRefGoogle Scholar
Petit, RJ, Mousadik, AE and Pons, O (1998) Identifying populations for conservation on the basis of genetic markers. Conservation Biology 12, 844855.CrossRefGoogle Scholar
Ribeiro-Carvalho, C, Guedes-Pinto, H, Igrjas, G, Stephenson, P, Schwarzacher, T, Heslop-Harrison, JS (2004) High levels of genetic diversity throughout the range of the Portuguese wheat landrace ‘Barbela’. Annals of Botany 94, 699705.CrossRefGoogle ScholarPubMed
Rohlf, FJ (2000) NTSYSpc Numerical Taxonomy and Multivariate Analysis System. Version 2.1. User Guide. Setauket, NY: Exeter Software.Google Scholar
StatSoft Inc. (2003) STATISTICA Release 7. Tulsa, OK: StatSoft Inc.Google Scholar
Teshome, A, Brown, AHD and Hodgkin, T (2001) Diversity in landraces of cereals and legume crops. In: Janick, J (ed.) Plant Breeding Reviews. New York: John Wiley & Sons, pp. 221261.CrossRefGoogle Scholar
Willcox, G and Tengberg, M (1995) Preliminary report on the archaeobotanical investigations at Tell Abraq with special attention to the chaff impressions in mud brick. Arabian Archaeology Epigraphy 6, 129138.CrossRefGoogle Scholar
Yu, J, Mosjidis, JA, Klingler, KA and Woods, FM (2001) Isozyme diversity in North American cultivated red clover. Crop Science 41, 16251628.CrossRefGoogle Scholar
Zhang, Q, Yang, GP, Dai, X and Sun, JZ (1994) A comparative analysis of genetic polymorphism in wild and cultivated barley from Tibet using isozyme and ribosomal DNA markers. Genome 37, 631638.CrossRefGoogle ScholarPubMed
Zohary, D and Hopf, M (1993) Domestication of Plants in the Old World: The Origin and Spread of Cultivated Plants in West Asia, Europe, and the Nile Valley, Oxford: Clarendon Press.Google Scholar