Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-26T09:14:26.052Z Has data issue: false hasContentIssue false
  • English
  • Français

Polymorphism of waxy proteins in Spanish hulled wheats

Published online by Cambridge University Press:  15 March 2011

C. Guzmán ,
L. Caballero ,
M. V. Gutierrez  and
J. B. Alvarez
C. Guzmán*
Affiliation:
Departamento de Genética, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, Edificio Gregor Mendel, Campus de Rabanales, Universidad de Córdoba, ES-14071 Córdoba, Spain
L. Caballero
Affiliation:
Departamento de Mejora Genética Vegetal, Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Apdo. 4084, ES-14080 Córdoba, Spain
M. V. Gutierrez
Affiliation:
Departamento de Genética, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, Edificio Gregor Mendel, Campus de Rabanales, Universidad de Córdoba, ES-14071 Córdoba, Spain
J. B. Alvarez
Affiliation:
Departamento de Genética, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, Edificio Gregor Mendel, Campus de Rabanales, Universidad de Córdoba, ES-14071 Córdoba, Spain
*
*Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Hulled wheats are neglected crops that have potential in plant breeding programmes of modern durum and common wheat. Among these wheats, three species were widely cultivated in Spain until the mid 20th century: Triticum monococcum ssp. monococcum (einkorn), Triticum turgidum ssp. dicoccum (emmer) and Triticum aestivum ssp. spelta (spelt). One important aspect of wheat grain quality is starch composition, which is related to the action of waxy proteins. A collection of 536 accessions of Spanish hulled wheats was analyzed for waxy protein composition using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Polymorphism was found for the Wx-A1, Wx-B1 and Wx-D1 proteins, including new and null alleles in the three species. An allelic variant with an electrophoretic mobility not previously described was found in einkorn wheat. In emmer and spelt, some alleles with different mobility were also found. A Wx-B1 null allele was detected in emmer wheat, and null alleles for Wx-A1, Wx-B1 and Wx-D1 were found in spelt wheat. The variations found could be used to enlarge the gene pool available to breeders, and to design new cultivars with different levels of amylose content.

Keywords

amylose contentgenetic polymorphismhulled wheatswaxy proteins
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 2 , July 2011 , pp. 330 - 333
Copyright
Copyright © NIAB 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ainsworth, CC, Clark, J and Balsdon, J (1993) Expression, organization and structure of the genes encoding the waxy protein (granule-bound starch synthase) in wheat. Plant Molecular Biology 22: 67–82.CrossRefGoogle ScholarPubMed
Alvarez, JB, Moral, A and Martín, LM (2006) Polymorphism and genetic diversity for the seed storage proteins in Spanish cultivated einkorn wheat (Triticum monococcum L. ssp. monococcum). Genetic Resources and Crop Evolution 53: 1061–1067.CrossRefGoogle Scholar
Caballero, L, Martín, LM and Alvarez, JB (2001) Allelic variation of the HMW glutenin subunits in Spain accessions of spelt wheat. Theoretical and Applied Genetics 103: 124–128.CrossRefGoogle Scholar
Caballero, L, Martín, LM and Alvarez, JB (2004a) Genetic variability of the low-molecular-weight glutenin subunits in spelt wheat (Triticum aestivum ssp. spelta L. em Thell.). Theoretical and Applied Genetics 108: 914–919.CrossRefGoogle ScholarPubMed
Caballero, L, Martín, LM and Alvarez, JB (2004b) Variation and genetic diversity for gliadins in Spanish spelt wheat accessions. Genetic Resources and Crop Evolution 51: 679–686.CrossRefGoogle Scholar
Caballero, L, Bancel, E, Debiton, C and Branlard, G (2008) Granule-bound starch synthase (GBSS) diversity of ancient wheat and related species. Plant Breeding 127: 548–553.CrossRefGoogle Scholar
Dvorak, J, Terlizzi, P, Zhang, HB and Resta, P (1993) The evolution of polyploidy wheats: identification of the A genome species. Genome 36: 21–31.CrossRefGoogle Scholar
Echt, CS and Schwartz, D (1981) Evidence for the inclusion of controlling elements within structural gene at the waxy locus in maize. Genetics 99: 275–284.CrossRefGoogle ScholarPubMed
Laemmli, UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.CrossRefGoogle ScholarPubMed
Pflüger, LA, Martín, LM and Alvarez, JB (2001) Variation in the HMW and LMW glutenin subunits from Spanish accessions of emmer wheat (Triticum turgidum ssp. dicoccum Schrank). Theoretical and Applied Genetics 102: 767–772.CrossRefGoogle Scholar
Rodríguez-Quijano, M, Nieto-Taladriz, MT and Carrillo, JM (1998) Polymorphism of waxy proteins in Iberian hexaploid wheats. Plant Breeding 117: 341–344.CrossRefGoogle Scholar
Yamamori, M, Nakamura, T, Endo, R and Nagamine, T (1994) Waxy protein deficiency and chromosomal location of coding genes in common wheat. Theoretical and Applied Genetics 89: 179–184.CrossRefGoogle ScholarPubMed
Yamamori, M, Nakamura, T and Nagamine, T (1995) Polymorphism of two waxy proteins in the emmer group of tetraploid wheat, Triticum dicoccoides, T. dicoccum, and T. durum. Plant Breeding 114: 215–218.CrossRefGoogle Scholar
Zeng, M, Morris, CF and Batey Wrigley, CW II (1997) Sources of variation for starch gelatinization, pasting, and gelation properties in wheat. Cereal Chemistry 74: 63–71.CrossRefGoogle Scholar