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Molecular distinction of two closely resembling Morinda species using rbcL and matK loci for quality management of Indian herbal medicines

Published online by Cambridge University Press:  29 November 2012

Rahul R. Nair ,
P. S. Udayan ,
S. Thilaga ,
M. Kavitha ,
R. M. Bharathanandhini ,
A. M. Nizzy  and
D. Ganesh
Rahul R. Nair
Affiliation:
Plant Genetic Improvement Laboratory, Department of Biotechnology, SPK Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi 627 412 Tirunelveli District, Tamil Nadu, India
P. S. Udayan
Affiliation:
Department of Botany, Sree Krishna College, Ariyannur 680 102, Thrissur District, Kerala, India
S. Thilaga
Affiliation:
Plant Genetic Improvement Laboratory, Department of Biotechnology, SPK Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi 627 412 Tirunelveli District, Tamil Nadu, India
M. Kavitha
Affiliation:
Plant Genetic Improvement Laboratory, Department of Biotechnology, SPK Centre for Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi 627 412 Tirunelveli District, Tamil Nadu, India
R. M. Bharathanandhini
Affiliation:
Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Palkalai Nagar Madurai 625 021, Tamil Nadu, India
A. M. Nizzy
Affiliation:
Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Palkalai Nagar Madurai 625 021, Tamil Nadu, India
D. Ganesh*
Affiliation:
Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Palkalai Nagar Madurai 625 021, Tamil Nadu, India
*
*Corresponding author. E-mail: [email protected]
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Abstract

Morinda reticulata Gamble and Morinda umbellata Linn. (Rubiaceae) are medicinally important climbers distributed as a mixed population in southern Western Ghats of India. A close morphological resemblance of these two species misleads the harvester in the identification of plant parts for preparation of herbal medicines. Though both species contain anthraquinone derivatives and share common medicinal properties for treating stomach disorders, each of these species has unique curative properties for treating selective diseases. Conventional methods are not reliable for identification of these species due to similarities in morphology. Thus, misidentification often leads to the deterioration of the quality of medicines. Thus, authentication utilizing conserved gene sequences in the chloroplast genome of these two Morinda spp. has been attempted for precise identification. Here we report the use of two barcoding genes (maturase kinase and ribulose 1,5-bisphosphate carboxylase large subunit) to distinguish M. reticulata and M. umbellata based on single nucleotide polymorphism. The present findings can be used for authenticating leaf samples of M. reticulata and M. umbellata.

Keywords

herbal medicinesM. reticulataM. umbellataquality managementRubiaceaesingle nucleotide polymorphism
Type
Short Communication
Information
Plant Genetic Resources , Volume 11 , Issue 1 , April 2013 , pp. 90 - 93
Copyright
Copyright © NIAB 2012

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