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In vitro culture and cultivation of Chinese medicinal plants for industrial utilization and genetic resource conservation

Published online by Cambridge University Press:  12 February 2007

Wenyuan Gao ,
Wei Jia ,
Xianfu Gao ,
Renfeng Wang  and
Peigen Xiao
Wenyuan Gao
Affiliation:
College of Pharmaceuticals & Biotechnology, Tianjin University, Tianjin, 300072, China
Wei Jia*
Affiliation:
School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200030, China
Xianfu Gao
Affiliation:
School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200030, China
Renfeng Wang
Affiliation:
School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200030, China
Peigen Xiao
Affiliation:
Institute of Medicinal Plant, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100094, China
*
*Corresponding author: E-mail: [email protected]
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Abstract

In China, medicinal plants enjoy an inherent and prominent role in the general health service. Due to excessive collection in the wild of rare and endangered plants, the natural resources of medicinal plants are depleting fast. In order to protect the medicinal plant resources, the Chinese government has implemented Good Agricultural Practice (GAP) programmes to cultivate the main popular medicinal plants in China. Thus far, around 800 GAP cultivation bases have been established nationwide and the total cultivation area of medicinal plants has reached 5000 km2. Besides GAP cultivation of medicinal plants, tissue cultural biotechnology has been applied to serve as an alternative for the supply of medicinal plant materials in China. Thus far, shoot production by tissue culture technology has been successful in medicinal plants such as Anoectochilus formosanus, Dalbergia odorifera, Dendrobium, Momordica grosvenorii, Pseudostellaria heterophylla and Taxus chinensis. In addition, the cell culture of Lithospermum erythrorhizon and Saussurea involucrata has been industrialized in 300–20,000-litre bioreactors. Besides the production of shoot and cell culture in bioreactors, tissue culture technology is also being practised for the conservation of rare medicinal plants.

Keywords

bioreactorcell and organ cultureGAP cultivationgenetic resource conservationmedicinal plant tissue culture
Type
Research Article
Information
Plant Genetic Resources , Volume 3 , Issue 2 , August 2005 , pp. 116 - 126
Copyright
Copyright © NIAB 2005

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References

An, Cui-hong, Li, Jian-guo, Jiang, Danet al. (2002) Study on the rapid propagation of pepper test-tube plantlet by micro-cuttage method. Journal of Liaoning Normal University (Natural Science Edition) 25(3): 296–297.Google Scholar
Asaka, I, Li, L and Hirotani, M (1993) Production of ginsenosides saponin by culturing ginseng (Panax notoginseng) embryogenic tissues in bioreactors. Biotechnology Letters 15(12): 1259–1264.Google Scholar
Bai, Jie, Liu, Jiang-xin, Tang, Linet al. (2003) The callus induction culture of Buddleja officinalis. Journal of Sichuan University (Natural Science Edition) 40(5): 958–962.Google Scholar
Cai, Jian-rong, Zeng, Jun, Zhang, Zhi-yonget al. (2002) Study on stem tissue culture and multiplication of Chinese Yam. Fujian Agricultural Science and Technology 2: 14–15.Google Scholar
Cao, Xiao-yan, Wa, Jie-zhi and Zhao, Yin-ping (2003) Anther culture and plant regeneration of Robinia hispida. Acta Botanica Boreali-occidentalia Sinica 23(3): 456–459.Google Scholar
Chen, Fang, Ying, Chun-rong and Guo, Yuan-linet al. (2003) Plantlet regeneration from calli of Bittergourd (Momordica charantia). Physiology Communications 39(6): 633Google Scholar
Chen, Gang, Jia, Jing-feng and Hao, Jian-guo (2003) Fertile plant regeneration from tissue culture in Allium mongolicum Regel. Bulletin of Botanical Research 23(1): 51–54.Google Scholar
Chen, Ting-su, Zhang, Jun and Xia, Ning-shaoet al. (2003) Plantlet regeneration from transverse thin-cell-layer culture of aloe. Subtropical Plant Science 32(2): 58Google Scholar
Chen, Yi-guang and Huang, Heng-yu (2002) Tissue culture of medical plant Swertia davidii Franch. Guihaia 22(5): 432–436.Google Scholar
Chen, Yong, Wang, Jun-hui and Huang, Chun-nong (2002) Cryopreservation by vitrification of carrot (Daucus carota L.) suspension cells and protoplasts. Journal of Zhejiang University (Sciences Edition) 29(1): 94–98.Google Scholar
Chen, Yong-qin, Fan, Jin-yu, Yi, Feiet al. (2004) Studies on plantlet regeneration from the mature leaves of Dioscorea zingiberensis. China Journal of Chinese Materia Medica 29(2): 129–132.Google Scholar
Dong, Ling, Chen, Jing-xian, Liao, Hua-junet al. (2003) Tissue culture and rapid propagation of Oenanthe stolonifera. Plant Physiology Communications 39(3): 235Google Scholar
Fan, Guo-qiang, Qu, Xiao-qiao and Li, Song-lin (2002) Plant regeneration from leaf calli of paulownia plants. Chinese Bulletin of Botany 19(1): 92–97.Google Scholar
Gao, Shan-lin and Chen, Bai-jun (2004) Optimization on cloning rapid-propagation technology by tissue culture in Scutellaria baicalensis. Chinese Traditional and Herbal Drugs 35(3): 312–315.Google Scholar
Gao, Shan-lin and Zhi, Hui (2004) Tissue culture of shoot tip and in vitro propagation of Chrysanthemum morifolium Ramat. Journal of Plant Resources and Environment 13(1): 24–27.Google Scholar
Guan, Wen-ling, Wang, Li and Zheng, Si-xiang (2003) Establishment of a plantlet regeneration system for Aconitum carmichaeli. Chinese Traditional and Herbal Drugs 34(6): 561–563.Google Scholar
Hao, Yu-rong and Li, Ming-shi (2002) Study on plant regeneration in cotyledon and hypocotyl segments of kyndyt. Acta Botanica Boreali-occidentalia Sinica 22(7): 82–84.Google Scholar
He, Hong (2003) A study on the tissue culture and plant regeneration of Basella rubra L. Primary Journal of Chinese Materia Medica 17(1): 11–12.Google Scholar
He, Li-jun and Yu, Zhuo (2003) Study on plant regeneration from explant culture and histological observation in the rare endangered plant Tetraena mongolica. Journal of Inner Mongolia Agriculture University (Natural Science Edition) 24(2): 27–32.Google Scholar
He, Shu-lan, Shu, Xiao-chun and Yao, Chun-juet al. (2003) Tissue culture of Lycoris radiata (L'Her.) herb. Journal of Jiangsu Forestry Science & Technology 30(4): 18–20.Google Scholar
Hsu, Tai-hua, Shiao, Li-hua, Hsieh, Chien-yan and Chang, Der-ming (2002) A comparison of the chemical composition and bioactive ingredients of the Chinese medicinal mushroom DongChongXiaCao, its counterfeit and mimic, and fermented mycelium of Cordyceps sinensis. Food Chemistry 78: 463–469.Google Scholar
Hu, Bo-ran, Xu, Wen-biao, Zhang, Shuang-linget al. (2003) Screening and characterization of hydroxyproline-resistant variants in Lycium barbarum and analysis of its salt tolerance. Acta Botanica Boreali-occidentalia Sinica 23(3): 422–427.Google Scholar
Hu, Chun-xia and Tang, Jie (2003) Tissue culture and regeneration of Aralia elata (Miq.) for plantlets in east Liaoning. Journal of Liaoning Teachers College (Natural Science Edition) 5(4): 96–97.Google Scholar
Hu, Wang-qun, Wang, Yang, Xu, Hong-binet al. (2003) Research about rapid propagation and discard virus of Solanum muricatum Ait. Journal of West Anhui University 19(5): 46–47.Google Scholar
Hu, Xin, Ding, Zhu-hong, Chen, Yi-junet al. (2002) Bioaccumulation of lanthanum and cerium and their effects on the growth of wheat (Triticum aestivum L.) seedlings. Chemosphere 48(6): 621–629.Google Scholar
Huang, Hui-lian, Liu, Xian-wang, Xie, Pinget al. (2002) Tissue culture of Gynura segetum. Chinese Traditional and Herbal Drugs 33(2): 159–160.Google Scholar
Huang, Luqi and Cui, Guang-hong (2002) Study on complex system of GAP implementation of Chinese Materia Medica. China Journal of Chinese Materia Medica 27: 1–3.Google Scholar
Huang, Ying, Fan, Yan-ping, Wang, Wen-shenget al. (2003) The induction of callus and plantlet regeneration in Dianthus chinensis. Journal of South China Agricultural University 24(1): 50–52.Google Scholar
Huang, Yuan-xin, He, Feng-fa, Yu, Yong-xionget al. (2004) Preliminary study of in vitro culture of peduncle of Artemisia annua L. Sichuan Grassland 4: 15–17.Google Scholar
Huang, Zhi-ming, She, Hui-min and Lin, Qing-liang (2002) A study on the industrial breeding technology of Dendrobium phalaenopsis. Journal of Putian University 9(3): 22–26.Google Scholar
Huo, Li-yun and Zheng, Xiao-jian (2002) Studies on tissue culture of Gentianella albifpra. Chinese Pharmaceutical Journal 37(6): 415–418.Google Scholar
Jia, Wei and Zhang, Lixin (2005) Chinese herbal drug industry: past, present and future. In: Zhang, L and Demain, A (eds) Natural Products: Drug Discovery, Therapeutics, and Preventive Medicines. Totowa, NJ Humana Press.Google Scholar
Jiang, Chang-yang, Ning, Shu-xiang, Wang, Menget al. (2003 a) Tissue culture and plantlet regeneration of Atriplex triangularis. Plant Physiology Communications 39(4): 347Google Scholar
Jiang, Chang-yang, Ning, Shu-xiang, Wang, Yuet al. (2003 b) A highly efficient plant regeneration system from callus of Pteridium apuilinum. Acta Horticulturae Sinica 30(3): 343–345.Google Scholar
Jiang, Lin, Ding, Ping and Zheng, Ying-dong (2003) Effects of additives on tissue culture and rapid propagation of Dendrobium canducum. Journal of Chinese Medicinal Materials 26(8): 539–541.Google Scholar
Jiang, Ru-lan, Zhang, Shi-jun, Zheng, Ying-donget al. (2002) Tissue culture and rapid propagation of Anthurium andraeanum Lind. Journal of Zhougkai Agrotechnical College 15(4): 49–53.Google Scholar
Jin, Hong, Jia, Jing-feng and Hao, Jian-guo (2002) Establishment of a highly efficient regeneration system in vitro and transformation mediated by Agrobacterium rhizogenes in Buckwheat (Fagopyrum esculentum). Acta Botanica Boreali-occidentalia Sinica 22(3): 611–616.Google Scholar
Jin, Hong, Jia, Jing-feng and Hao, Jian-guo (2004) Protoplast culture and plant regeneration of the methionine resistant variant of Astragalus melilotoides Pall. Chinese Journal of Biotechnology 20(3): 221–226.Google ScholarPubMed
Lei, Ying (2003 a) Tissue culture and rapid propagation of Chamaenerion angustifolium. Plant Physiology Communications 39(4): 354Google Scholar
Lei, Ying (2003 b) Tissue culture and rapid propagation of Hypericum ascyron. Gansu Agricultural Science and Technology 38(3): 357–360.Google Scholar
Li, Guo-ping and Yang, Lu-sheng (2002) Tissue culture and plantlet regeneration of Hedyotis diffusa. Plant Physiology Communications 38(2): 150Google Scholar
Li, Hui-ning and An-yu, Yang Xin (2002) In vitro culture and rapid propagation of Hypericum perforatum. Chinese Wild Plant Resources 21(4): 61–63.Google Scholar
Li, Jing, Jia, Jing-feng and Hao, Jian-guo (2003) Tissue culture and plant regeneration of Peganum harmala. Acta Botanica Boreali-occidentalia Sinica 23(3): 428–432.Google Scholar
Li, Qing and Su, Xue-hen (2003) Forsythia koreanna ‘SauonGold’ rooting in a tube and survival rate of transplantation. Journal of Beijing Forestry University 2(1): 58–61.Google Scholar
Li, Rui (2003) Tissue culture for rapid propagation of Lilium longiflorun. Journal of Gansu Forestry Science and Technology 28(2): 13–14, 30Google Scholar
Li, Rui-fen, Zhang, Jing-yuan and Zhao, Mao-lin (2003) Embryogenic callus induction and plant regeneration of Zoysia japonica. Acta Horticulturae Sinica 30(3): 355–357.Google Scholar
Li, Shi-jun, Liu, Qin, Wang, Guang-donget al. (2002) Effect of different hormones with various concentrations on apical bud proliferation of Wasabi (Wasabi japonica Matsum). Southwest China Journal of Agricultural Sciences 15(3): 66–69.Google Scholar
Li, Tao and Wang, Zheng-zhi (2003) Establishment of a regeneration system for Portulace deracea L.. Journal of Shaanxi Normal University (Natural Science Edition) 31(2): 95–98.Google Scholar
Li, Zheng-hong, Sun, Zhen-yuan and Li, Xing-xin (2003) Tissue culture and plantlet regeneration of Parthenocissus tricuspidata. Plant Physiology Communications 39(5): 482Google Scholar
Liang, Peng and Shi, He-ping (2003) Plantlet regeneration from leaf explants of Zamioculcas zamiifolia. Acta Horticulturae Sinica 30(5): 621–622.Google Scholar
Lin, Bi-ying and Lin, Yi-zhang (2003) Tissue culture and rapid propagation of Gynura bicolor. Plant Physiology Communications 39(4): 346Google Scholar
Ling, Zheng-zhu, Wu, Qing-hua and Lan, Zu-zai (2003) The tissue culture of Blumea aromatica. Lishizhen Medicine and Materia Medica Research 14(1): 4–7.Google Scholar
Liu, Bin, Liu, Xuan-ming and Wang, Xiao-rong (2003) The control of different hormones on clonal propagation in vitro of momordicacharantia. Journal of Natural Science of Huanan Normal University 26(4): 76–78.Google Scholar
Liu, Cui-ping, Jin, Guo-liang, Song, Jing-yuanet al. (2003) Establishment of the asexual system of carnation. Journal of Hunna University of Arts and Science (Natural Science Edition) 15(4): 29–31.Google Scholar
Liu, CZ, Murch, SJ, EL-Demerdash, Met al. (2004) Artemisia judaica L.: micropropagation and antioxidant activity. Journal of Biotechnology 110(1): 63–71.Google Scholar
Liu, Xue-an and Wang, Yong-qing (2003) A study on in vitro culture techniques of Zhongjiang large-leaf type Salvia. Journal of Sichuan Agricultural University 21(1): 73–75.Google Scholar
Liu, Yong and Liu, Xian-wang (2004) Briefing of Dendrobium herooglossum seedling culture. Journal of Jiangxi University of TCM 16(1): 54Google Scholar
Lou, Lai-qing, Shen, Zhen-guo and Li, Xiao-dong (2004) The copper tolerance mechanisms of Eisholtzia haichowensis, a plant from copper-enriched soils. Environment and Experimental Botany 51(2): 111–120.Google Scholar
Lu, Li-tang, Zhu, Dong-xue and Zhao, De-gang (2003) Rapid plantlet regeneration of Camptotheca acuminata. Molecular Plant Breeding 1(5): 829–830.Google Scholar
Lu, Shi-an, Shen, Yan-fen, Huang, Yuan-xunet al. (2003) Study on Amorphophallus rivieri tissue culture and fast propagating the grandparent seed techniques with test tube culture. Hubei Agriculture Sciences 6: 68–70.Google Scholar
Lu, Tian-ling, Zhou, Chuan-ming, Huang, Shou-xianet al. (2002) Tissue culture and rapid propagation of medicinal plant Stemona japonica. Chinese Traditional and Herbal Drugs 33(9): 843–846.Google Scholar
Luo, Guang-ming, Yang, Ya-qin, He, Yan (2003) Studies on fast propagation of Pinellia ternate. Chinese Traditional Medicinal Material 26(10): 699–701.Google Scholar
Luo, Wei-wei Hu, Qing, Duan Xue-tao (2003) Tissue culture of Agave americana cv. marginata. Jiangxi Forestry Science and Technology 6: 10–11.Google Scholar
Luo, Peng and Zhou, Song-dong (2002) Studies on factors of high rate regeneration of plants from Descurainia sophia hypocotyl. Acta Botanica Yunnanica 24(4): 515–520.Google Scholar
Ma, Hai-yun He, Ming-zhu, and, Li, Yi (2002) Induction, differentiation and plant regeneration of triploid calli of Populus tomentosa. Bulletin of Botanical Research 22(3): 288–291.Google Scholar
Ma, Huai-yu, Li, Ya-dong, Liu, Qing-zhonget al. (2004) Regeneration of highbush blueberry plantlets from leaf section. Journal of Northeast Agricultural University 35(2): 212–215.Google Scholar
Ma, Jian-sheng, Zeng, Fu-hua, Lan, Hai-tinget al. (2004) Tissue culture and plantlet regeneration of common centipedegrass. Plant Physiology Communications 40(1): 62Google Scholar
Ma, Yan, Xiao, Ya-ping and Hu, Ya-qin (2003) Tissue culture and plant regeneration in Celastrus angulatus. Chinese Traditional and Herbal Drugs 34(10): 4–7.Google Scholar
Mo, Rao, Zhu, Wen-li, Wu, Fan-huaet al. (2003) In vitro culture of Amomum longiligulare. Chinese Journal of Tropical Agriculture 23(4): 1–4.Google Scholar
Ni, Su (2003) Tissue culture and plantlet regeneration of Gaillardia pulchella. Plant Physiology Communications 39(4): 342Google Scholar
Pan, Chao-guan, He, Hong, Xiao, Sheng-eet al. (2003) Effects of crude extractive on growth of Pogostemon cablin (Blanco) Benth. plantlets bred in vitro. Journal of Guangzhou University of Traditional Chinese Medicine 20(1): 70–72.Google Scholar
Piao, Ri-zi, Cao, Hou-nan, Chen, Yan-qiuet al. (2003) Study on the in vitro cell technology of Saussurea inuol uorata kar, etkir. Journal of Agricultural Science Yanbian University 25(2): 117–121.Google Scholar
Qin, LP, Huang, BK, Zheng, HC (2001) The background, present situation, problem and tactics of GAP of Chinese material medica. Yaoxue Shijian Zazhi 19: 67–70.Google Scholar
Sha, Hong, Liao, Kang (2003 a) Study on tissue culture in medicinal herb Mentha spicata L. Journal of Xinjiang Agricultural University 26(1): 10–12.Google Scholar
Sha, Hong, Liao, Kang (2003 b) Study on tissue culture in medicinal herb Origanum vulgare L. Journal of Xinjiang Agricultural University 26(4): 49–51.Google Scholar
Shang, MF, Zhu, Y (2000) The situation of cultivation base of TCM in China. Research & Information on Traditional Chinese Medicine 2(10): 23–27.Google Scholar
Shen, Ning-dong, Tang, Rong and Wei, Mei-qin (2003) Root formation on tissue culture of test-tube shoots of Gypsophila paniculata. Journal of Qinghai Normal University (Natural Science Edition) 2: 62–64.Google Scholar
Sheng, Wei, Wang, Yue-hui, Zhang, Ai-minet al. (2003) Study on plant tissue culture of Cornus officinalis. China Journal of Chinese Materia Medica 28(2): 118–121.Google Scholar
Shi, He-ping and Huang, Qun-sheng (2003) Tissue culture and plantlet regeneration from leaves of Nicotiana tabacum. Subtropical Plant Science 32(4): 63Google Scholar
Shi, He-ping and Qi, Yin (2004) Tissue culture and plantlet regeneration from leaves of Euphorbia pulcherrima. Subtropical Plant Science 33(1): 56Google Scholar
Shi, Wei, Luo, Jian-ping and Huang, Xiu-yan (2003) Effect of growth regulators on rooting of regenerated shoots from Dendrobium huoshanense protocorm-like bodies. Chinese Traditional and Herbal Drugs 34(10): 954–957.Google Scholar
Song, Xi-quan and Song, Qin-qu (2003) Regeneration of green plants by means of stem section culture on Dendrobium nobile Lindl. Journal of Guizhou Normal University (Natural Science) 21(3): 80–82.Google Scholar
Su, Cheng-gang and Zhang, Xing-guo (2003) Studies on tissue culture and plant regeneration of Amorphophallus dunnii Tetcher. Journal of Southwest Agriculture University 25(5): 393–395.Google Scholar
Su, Ge-er and Bao, Ying-hua (2002) Study on the tissue culture of Astragalus membranaceus Bge. var. mongholicus (Bge.) Hisao. Journal of Shaoguan University 239: 70–76.Google Scholar
Sun, Shi-hai, Liu, Yu-qin, Wang, Zhen-xinget al. (2003) Study on fast propagation of cymbidium with tissue culture and test-tube seedling transplants. Journal of Tianjin Agricultural College 10(4): 33–37.Google Scholar
Tan, Xian-he, Chao, Jian-guo, Zhang, Yuet al. (2003) Research on fast-propagation of Pseudostellaria heterophylla. Journal of Chinese Medicinal Materials 26(2): 82–83.Google ScholarPubMed
Tang, De-ying, Wang, Yun-qiang, Li, Xue-lanet al. (2003) Rapid micropropagation of Dicorea composita. Tropical Agricultural Science & Technology 26(1): 16–18.Google Scholar
Tang, Wei, Luo, Xiao-yan, Samuels, V (2002) Growth and differentiation of transgenic callus regulated by phytohormones and antibiotics in transformation of loblolly pine. Acta Genetica Sinica 29(2): 166–174.Google Scholar
Teng, Nian-jun and Chen, Fa-li (2003) Tissue culture of Callistephus chinensis. Plant Physiology Communications 39(3): 277Google Scholar
Tian, Zhi-hong and Yan, Han (2003) Tissue culture and plantlet regeneration of Dichondra repens. Plant Physiology Communications 39(5): 481Google Scholar
Wang, Jian-wen and Tan, Ren-xiang (2002) Tissue culture and plantlet regeneration of Artemisia princeps. Plant Physiology Communications 38(1): 38Google Scholar
Wang, JM, Liu, HM, Jiang, CZ and Ren, QH (2002) The establishment of GAP cultivation base. Zhongguo Yaoshi 16: 32–34.Google Scholar
Wang, Li and Li, Chun-yan (2003) Effects of the combination of plant hormones and the concentrations of inorganic salts on the rooting of tissue cultured-seedlings of tetraploid Robinia pseudoacacia L. Journal of Jiangsu Forestry Science & Technology 30(6): 23–25.Google Scholar
Wang, Li and Long, Chun-lin (2003) Tissue culture of Boea hygrometrica. Plant Physiology Communications 39(3): 233Google Scholar
Wang, Li, Liu, Guang-yong and Li, Qun (2004) Effect of hormone on plantlet regeneration and studies of root regeneration culture of Eustoma sp. Guihaia 24(1): 40–42.Google Scholar
Wang, Li-yan and Liang, Guo-lu (2004) Culture in vitro of Lily and rapid propagation of regenerated plantlets. Guizhou Agricultural Sciences 31(6): 19–21.Google Scholar
Wang, LX and Xiao, LL (2000) Significance of medicinal botany to the conservation of endangered species. In: Zhang, ED and Zheng, HC (eds) Conservation of Endangered Medicinal Wildlife Resources in China. Shanghai: Second Military Medical University Press, 83–86.Google Scholar
Wang, Ri-zhao and Wang, Zhi-an (2002) Selecting new species of Dioscorea zingiberensis containing high saponin by tissue culture. Chinese Traditional and Herbal Drugs 33(4): 361–363.Google Scholar
Wang, Xiao-juan, Jin, Liang, Shen, Yan-songet al. (2002) Root induced in callus culture of Hemerocallis hybrida. Journal of Fudan University (Natural Science) 41(1): 89–91, 96Google Scholar
Wang, Yu-fu and Dai, Jian-ping (2004) Plant regeneration through Linum usitatissimum L. anther culture. Plant Fibers and Products 26(1): 49–52.Google Scholar
Wu, Ai-zhong, He, Huan-le, Liu, Yan-fanget al. (2004) Propagation in vitro and karyotype analysis of Armeria maritime. Guihaia 24(1): 33–36. 60Google Scholar
Wu, Gang (2002) Adventitious shoot regeneration of Sinnigia hybrida Hort. young leaf explants. Journal of Jiangsu Forestry Science & Technology 29(3): 356Google Scholar
Wu, Jin-shou, Lin, Qing-liang, Lin, Shun-quanet al. (2004) Plant regeneration in vitro and industrialization of seedling production from pistil with pedicle in Rhododendron hybridum. Journal of Xiamen University (Natural Science) 43(1): 128–132.Google Scholar
Wu, Qing, Yan, Yong and Liang, Guo-yong (2002) Callus induction and plant regeneration from endosperm culture of Averrhoa carambola. Chinese Journal of Tropical Crops 23(2): 54–57.Google Scholar
Wu, Wei, Zheng, You-liang, Liu, Fanet al. (2004) Construction of a fast propagation system for Houttuynia cordata new line. China Journal of Chinese Materia Medica 29(1): 24–28.Google Scholar
Xiang, Yang, Zhao, De-gang and Zhu, Dong-xue (2003) Leaf culture in vitro of Eucommia ulmoides and rapid propagation of regenerated plantlet. Molecular Plant Breeding 1(5): 825–826.Google Scholar
Xiao, Jin-he Wu, Da-chun, Qin Ya-ping (2004) Study on tissue culture and quick reproduction technology of Aralia mandshurica. Journal of Anhui Agricultural Sciences 32(1): 111–114.Google Scholar
Xiong, Zhi-ting Li, Ying-hong, Xu Bin (2000) Nutrition influence on copper accumulation by Brassica pekinensis Rupr. Ecotoxicology and Environment Safety 53(2): 200–205.Google Scholar
Xu, Da-cheng, Liu, Xiao-yan, Cao, Weiet al. (2004) Influence of several plant growth substances on propagation of GGB of Cymbidium grandiflorum. Bulletin of Botanical Research 24(1): 76–77.Google Scholar
Xu, Pin-san, Luan, Yu-shi, Liu, Ji-wenet al. (2003) Study on production of virus-free bulblets by adventitious bud culture in Lilium spp. Chinese Bulletin of Botany 20(3): 313–318.Google Scholar
Xu, Qi-jiang, Yang, Shou-zhi, Chi, Chun-yuet al. (2003) Study on micropropagation through bulblet formation in tillered-onion. Chinese Bulletin of Botany 20(1): 80–84.Google Scholar
Xue, Hao, Chen, Zhong-li, Yan, Zhi-lianet al. (2003) Primary study on establishment of tissue culture seedling clones of aloe. Journal of Yanan University (Natural Science Edition) 22(2): 62–64.Google Scholar
Xue, Jian-ping, Zhang, Ai-min and Cheng, Jian-ping (2002) Study on callus induction and rapid propagation of Rubia cordifolia. China Journal of Chinese Materia Medica 27(2): 101–103.Google Scholar
Xue, Jian-ping, Wang, Ruo-yi, Zhang, Ai-minet al. (2003) Studies on organ phytomorphology of leaves from Rehmannia glutinosa. China Journal of Chinese Materia Medica 28(1): 21–24.Google Scholar
Xue, Jian-ping, Chang, Wei and Zhang, Ai-min (2004 a) Studies on the technology of directly inducing regenerated plantlet from leaf of Chrysanthemum morifolium. China Journal of Chinese Materia Medica 29(2): 132–135.Google Scholar
Xue, Jian-ping, Chang, Wei, Zhang, Ai-minet al. (2004 b) Studies on petal tissue culture of the traditional Chinese medicine Chrysanthemum morifolium. China Journal of Chinese Materia Medica 29(3): 211–214.Google Scholar
Xun, Xiao-hong, Jiang, Tai-wen, Peng, Xiao-yinget al. (2003) Studies on the ways of shoot regeneration and the technique to induce polyploids of Artemisia annua. Journal of Hunan Agricultural University (Natural Sciences) 29(2): 115–119.Google Scholar
Yang, Ben-peng (2003) Tissue culture of Dendrocalamus giganteus. Chinese Journal of Tropical Crops 24(3): 82–87.Google Scholar
Yang, Bo Li, Hong-lin, Long Yun-ming (2003) Tissue culture of Echinocodon lobophyllus Hong. Journal of Wuhan Botanical Research 21(5): 457–458.Google Scholar
Yang, Pei-jun Li, Hui-ning, Zhao Hua (2003) Tissue culture and rapid propagation of Reynoutria japonica. Acta Botanica Boreali-occidentalia Sinica 23(2): 2192–2195.Google Scholar
Yang, Yao-wen, Qian, Zi-gang, Xie, Huiet al. (2003) Tissue culture and rapid breeding of the rare and endangered medicinal plant Psammoseline tunicoides. World Science and Technology—Modernization of Traditional Chinese Medicine and Materia Medica 5(4): 56–59.Google Scholar
Yang, Yong-hua (2003) Studying on tissue culture techniques for Hemerocallis middendorfii, Golden boy. Gansu Agricultural Science and Technology 12: 33–35.Google Scholar
Yi, Yin and Zhang, Yu-bin (2004) Tissue culture and plantlet regeneration of Dendrobium loddigesii. Plant Physiology Communications 40(1): 64Google Scholar
Yoshikawa, T, Furuya, T (1987) Saponin production by cultures of Panax ginseng transformed with Agrobacterium rhizogenes. Plant Cell Reports 6: 449–453.Google Scholar
Yuan, CQ, Wang, NH and Lu, Y (2000) Conservation of endangered medicinal plants in China. In: Zhang, ED and Zheng, HC (eds) Conservation of Endangered Medicinal Wildlife Resources in China. Shanghai: Second Military Medical University Press, 25–32.Google Scholar
Zeng, Shen (2003) Tissue culture and rapid propagation of Zanthoxylum piperitum. Plant Physiology Communications 39(4): 353Google Scholar
Zhang, Guang-fei and Su, Wen-hua (2002) Tissue culture of Neottopteris antrophyoides. Plant Physiology Communications 38(2): 144Google Scholar
Zhang, Guo-yu, Cheng, Zhi-hui, Li, Juanet al. (2004) Establishment of adventitious bud induction and a highly efficient propagation system in many flower betony (Stachys floridana Schuttl. Ex Benth.). Journal of the Northwest, University of Agricutural and Forestry Science and Technology (Natural Science Edition) 32(2): 49–52.Google Scholar
Zhang, Heng-tao, Li, Jing, Song, Shang-weiet al. (2004) Tissue culture and plantlet regeneration of dwarf Prunus persica rootstock. Journal of Henan Agricultural University 38(1): 77–81.Google Scholar
Zhang, Song, Da, Ke-dong, Cao, Zhen-xinget al. (2002) Rapid micropropagation system via in vitro culture in Amaryllis vittata and its embryogenesis. Acta Horticulturae Sinica 29(3): 285–287.Google Scholar
Zhang, Xiu-shen, Li, Ya-jie, Du, Xing-fanet al. (2002) Tissue culture of Aloe saponaria. Biotechnology 12(1): 28Google Scholar
Zhang, Zi-xue, Ding, Wei-qun, Tang, Yonget al. (2004) Study on tissue culture of pasqueflower. China Journal of Chinese Materia Medica 29(3): 215–218.Google Scholar
Zhao, Jun, Wang, Hua, Pan, Jun-songet al. (2004) In vitro culture and plantlet regeneration from cotyledonary nodes of cucumber (Cucumis sativus L.). Journal of Shanghai Jiao Tong University (Agricultural Science) 22(1): 43–47, 53Google Scholar
Zheng, Hai-rou, Ye, Zheng-wen, Su, Ming-shenet al. (2004) Induction of mutants in jujube in vitro culture by 60 Co γ-ray irradiation. Acta Agriculturae Shanghai 20(1): 13–18.Google Scholar
Zhou, RH (2001) Improve the process of GAP application in TCM in China. Foreign Medicine: Plant Drugs 16: 5–7.Google Scholar
Zhou, Yan-qing, Zhang, Gen-fa, Jia, Jing-fenget al. (2003) Factors influencing protoplast culture of the seedlings of Cassia obtusifolia (Leguminosae). Guihaia 23(4): 334–338.Google Scholar
Zhu, Gen-fa Ye, Xiu-bin, Chen Ming-li (2003) Effects of different ingredients of culture medium on the plant regeneration of rhizomes from Cymbidium sinense. Journal of Central South Forestry University 23(5): 42–44, 58Google Scholar
Zhu, YG, Huang, YZ, Hu, Yet al. (2003) Iodine uptake by spinach (Spinacia oleracea L.) plants grown in solution culture: effect of iodine species and solution concentrations. Environment International 29(1): 33–37.Google Scholar
Zou, TC (2001) Studies on investigation and utilization of medicinal plant germplasm resources for sustainable development in Guizhou, China. Journal of Guizhou Normal University (Natural Sciences) 19: 1–11.Google Scholar