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Identification of root morphology mutants in barley

Published online by Cambridge University Press:  16 March 2011

Riccardo Bovina ,
Valentina Talamè ,
Matteo Ferri ,
Roberto Tuberosa ,
Beata Chmielewska ,
Iwona Szarejko  and
Maria Corinna Sanguineti
Riccardo Bovina
Affiliation:
Department of Agroenvironmental Science and Technology (DiSTA), University of Bologna, Italy
Valentina Talamè
Affiliation:
Department of Agroenvironmental Science and Technology (DiSTA), University of Bologna, Italy
Matteo Ferri
Affiliation:
Department of Agroenvironmental Science and Technology (DiSTA), University of Bologna, Italy
Roberto Tuberosa
Affiliation:
Department of Agroenvironmental Science and Technology (DiSTA), University of Bologna, Italy
Beata Chmielewska
Affiliation:
Department of Genetics, University of Silesia, Poland
Iwona Szarejko
Affiliation:
Department of Genetics, University of Silesia, Poland
Maria Corinna Sanguineti*
Affiliation:
Department of Agroenvironmental Science and Technology (DiSTA), University of Bologna, Italy
*
*Corresponding author. E-mail: [email protected]
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Abstract

In this study, a forward-genetics analysis was performed on a portion of TILLMore, a chemically mutagenized population of barley cv. ‘Morex’ (http://www.distagenomics.unibo.it/TILLMore/), to identify root morphology alterations by comparison with ‘Morex’ wild-type. For this purpose, a simple paper-roll approach was performed to identify phenotypic variants at the seedling stage. The analysis of c. 1000 M4 families allowed us to identify c. 70 lines with altered root morphology. A more accurate phenotypic characterization of a portion of the mutant lines has been performed using stereomicroscopy and a scanning electron microscopy approach.

Keywords

forward-geneticsHordeum vulgareroot hairsroot morphologyTILLING
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 2 , July 2011 , pp. 357 - 360
Copyright
Copyright © NIAB 2011

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References

Chen, B, Roos, P, Borggaard, OK, Zhu, YG and Jakobsen, I (2005) Mycorrhiza and root hairs in barley enhance acquisition of phosphorus and uranium from phosphate rock but mycorrhiza decreases root to shoot uranium transfer. Plant Phytology 165: 591–598.Google Scholar
Coudert, Y, Périn, C, Courtois, B, Khong, NG and Gantet, P (2010) Genetic control of root development in rice, the model cereal. Trends in Plant Science 15: 219–226.Google Scholar
de Dorlodot, S, Forster, B, Pagès, L, Price, A, Tuberosa, R and Draye, X (2007) Root system architecture: opportunities and constraints for genetic improvement of crops. Trends in Plant Science 12: 474–481.Google Scholar
Eapen, D, Barroso, ML, Campos, ME, Ponce, G, Corkidi, G, Dubrovsky, JG and Cassab, GI (2003) A no hydrotropic response root mutant that responds positively to gravitropism in Arabidopsis. Plant Physiology 131: 536–546.CrossRefGoogle ScholarPubMed
Engvild, KC and Rasmussen, SK (2004) Root hair mutants of barley. Barley Genetics Newsletter 34: 13–15.Google Scholar
Gahoonia, TS, Nielsen, NE, Joshi, PA and Jahoor, A (2001) A root hairless barley mutant for elucidating genetic of root hairs and phosphorus uptake. Plant and Soil 235: 211–219.Google Scholar
Hochholdinger, F and Tuberosa, R (2009) Genetic and genomic dissection of maize root development and architecture. Current Opinion in Plant Biology 12: 172–177.Google Scholar
Hodge, A, Berta, G, Doussan, C, Merchan, F and Crespi, M (2009) Plant root growth, architecture and function. Plant and Soil 321: 153–187.Google Scholar
Kobayashi, A, Takahashi, A, Kakimoto, Y, Miyazawa, Y, Fujii, N, Higashitani, A and Takahashi, H (2007) A gene essential for hydrotropism in roots. Proceedings of the National Academy of Science USA 104: 4724–4729.Google Scholar
Kwasniewski, M and Szarejko, I (2006) Molecular cloning and characterization of beta-expansin gene related to root hair formation in barley. Plant Physiology 141: 1149–1158.CrossRefGoogle ScholarPubMed
Massa, GD and Gilroy, S (2003) Touch modulates gravity sensing to regulate the growth of primary roots of Arabidopsis thaliana. Plant Journal 33: 435–445.Google Scholar
McCallum, CM, Comai, L, Greene, EA and Henikoff, S (2000) Targeting Induced Local Lesion IN Genomes (TILLING) for plant functional genomics. Plant Physiology 123: 439–442.Google Scholar
Nawrot, M, Szarejko, I and Maluszynski, M (2005) Barley mutants with short roots. Barley Genetics Newsletter 35: 3–8.Google Scholar
Richards, RA (2008) Genetic opportunities to improve cereal root system for dryland agriculture. Plant Production Science 11: 12–16.Google Scholar
Shao, HB, Chu, LY, Jaleel, CA and Zhao, CX (2008) Water-deficit stress-induced anatomical changes in higher plants. Comptes Rendus Biologies 331: 215–225.CrossRefGoogle ScholarPubMed
Szarejko, I, Janiak, A, Chmielewska, B and Nawrot, M (2005) Genetic analysis of several root hair mutants of barley. Barley Genetics Newsletter 35: 36–38.Google Scholar
Talamè, V, Bovina, R, Sanguineti, MC, Tuberosa, R, Lundqvist, U and Salvi, S (2008) TILLMore, a resource for the discovery of chemically induced mutants in barley. Plant Biotechnology Journal 6: 477–485.Google Scholar
Talamè, V, Bovina, R, Salvi, S, Sanguineti, MC, Piffanelli, P, Lundquist, U and Tuberosa, R (2009) TILLING with TILLMore. In: Shu, QY (ed.) Induced Plant Mutations in the Genomics Era. Joint FAO/IAEA Programme. Rome: Food and Agriculture Organization of the United Nations, pp. 240–242.Google Scholar
Taramino, G, Sauer, M, Stauffer, JL Jr, Multani, D, Niu, X, Sakai, H and Hochholdinger, F (2007) The maize (Zea mays L.) RTCS gene encodes a LOB domain protein that is a key regulator of embryonic seminal and post-embryonic shoot-borne root initiation. Plant Journal 50: 649–659.Google Scholar
Woll, K, Borsuk, LA, Stransky, H, Nettleton, D, Schnable, PS and Hochholdinger, F (2005) Isolation, characterization, and pericycle-specific transcriptome analyses of the novel maize lateral and seminal root initiation mutant rum1. Plant Physiology 139: 1255–1267.Google Scholar