Hostname: page-component-586b7cd67f-rdxmf Total loading time: 0 Render date: 2024-11-22T04:09:18.493Z Has data issue: false hasContentIssue false
  • English
  • Français

Genotypic variation in leaf epicuticular wax quantity in a large faba bean (Vicia faba L.) germplasm collection

Published online by Cambridge University Press:  04 December 2018

Hamid Khazaei Open the ORCID record for Hamid Khazaei [Opens in a new window] ,
Arja Santanen ,
Kenneth Street  and
Frederick L. Stoddard Open the ORCID record for Frederick L. Stoddard [Opens in a new window]
Hamid Khazaei*
Affiliation:
Department of Agricultural Sciences, Viikki Plant Science Centre, Helsinki Sustainability Science Centre, University of Helsinki, Helsinki, Finland
Arja Santanen
Affiliation:
Department of Agricultural Sciences, Viikki Plant Science Centre, Helsinki Sustainability Science Centre, University of Helsinki, Helsinki, Finland
Kenneth Street
Affiliation:
International Center for Agricultural Research in the Dry Areas (ICARDA), Rabat, Morocco
Frederick L. Stoddard
Affiliation:
Department of Agricultural Sciences, Viikki Plant Science Centre, Helsinki Sustainability Science Centre, University of Helsinki, Helsinki, Finland
*
*Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Among grain legumes, faba bean is reputed to be relatively sensitive to drought stress. Epicuticular wax (ECW) quantity is considered as an important drought adaptation strategy in plant species. This study aimed to define variation in leaf ECW concentration as a drought-adaptive trait in 197 faba bean accessions under well-watered conditions. The relationship between ECW and stomatal characteristics was also investigated. Highly significant differences were found in the ECW concentration, which ranged from 0.680 to 2.104 mg/dm2. No relationships were found between ECW and any measure of stomatal morphology and function. This study provides evidence of the wide variation in ECW in faba bean germplasm, which is independent of stomatal characteristics and leaf water content. This variation may allow the genetic improvement of ECW as a drought-adaptive character in faba bean breeding programs aiming at the economical use of water.

Keywords

droughtepicuticular waxfaba beangenetic diversitystomata
Type
Short Communication
Information
Plant Genetic Resources , Volume 17 , Issue 3 , June 2019 , pp. 298 - 300
Copyright
Copyright © NIAB 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

†

Present address: Department of Plant Sciences, University of Saskatchewan, Saskatoon, Canada.

References

Barrs, HD and Weatherley, PE (1962) A re-examination of the relative turgidity technique for estimating water deficit in leaves. Australian Journal of Biological Sciences 15: 413–428.Google Scholar
Belachew, KY, Nagel, KA, Fiorani, F and Stoddard, FL (2018) Diversity in root growth responses to moisture deficit in young faba bean (Vicia faba L.) plants. PeerJ 6: e4401.Google Scholar
Darwish, DS and Fahmy, GM (1997) Transpiration decline curves and stomatal characteristics of faba bean genotypes. Biologia Plantarum 39: 243–249.Google Scholar
Daryanto, S, Wang, L and Jacinthe, P-A (2015) Global synthesis of drought effects on food legume production. PLoS ONE 10: e0127401.Google Scholar
Ebercon, A, Blum, A and Jordan, WR (1977) A rapid colorimetric method for epicuticular wax content of sorghum leaves. Crop Science 17: 179–180.Google Scholar
Figueiredo, KV, Oliveira, MT, Oliveira, AFM, Silva, GC and Santos, MG (2012) Epicuticular-wax removal influences gas exchange and water relations in the leaves of an exotic and native species from a Brazilian semiarid region under induced drought stress. Australian Journal of Botany 60: 685–692.Google Scholar
Huggins, T, Mohammed, S, Sengodon, P, Ibrahim, AMH, Tilley, M and Hays, DB (2018) Changes in leaf epicuticular wax load and its effect on leaf temperature and physiological traits in wheat cultivars (Triticum aestivum L.) exposed to high temperature during anthesis. Journal of Agronomy and Crop Science 204: 49–61.Google Scholar
Jenks, MA and Ashworth, EN (1999) Plant epicuticular waxes: function, production and genetics. In: Janick, J (ed.) Horticultural Reviews. Oxford, UK: John Wiley & Sons, Inc., pp. 1–68.Google Scholar
Khan, HR, Paull, JG, Siddique, KHM and Stoddard, FL (2010) Faba bean breeding for drought affected environments: a physiological and agronomic perspective. Field Crops Research 115: 279–286.Google Scholar
Khazaei, H, Street, K, Bari, A, Santanen, A and Stoddard, FL (2013) Do faba bean (Vicia faba L.) accessions from environments with contrasting seasonal moisture availabilities differ in stomatal characteristics and related traits? Genetic Resources and Crop Evolution 60: 2343–2357.Google Scholar
Khazaei, H, Link, W, Street, K and Stoddard, FL (2018) ILB 938, a valuable faba bean (Vicia faba L.) accession. Plant Genetic Resources: Characterization and Utilization 16: 478–482.Google Scholar
Kim, KS, Park, SH, Kim, DK and Jenks, MA (2007) Influence of water deficit on leaf cuticular waxes of soybean (Glycine max [L.] merr.). International Journal of Plant Sciences 168: 307–316.Google Scholar
McDonald, GK and Paulsen, GM (1997) High temperature effects on photosynthesis and water relations of grain legumes. Plant and Soil 196: 47–58.Google Scholar
Ouyang, W, Struik, PC, Yin, X and Yang, J (2017) Stomatal conductance, mesophyll conductance, and transpiration efficiency in relation to leaf anatomy in rice and wheat genotypes under drought. Journal of Experimental Botany 68: 5191–5205.Google Scholar
R Development Core Team (2016) R: A language and environment for statistical computing. R foundation for statistical computing, Vienna, Austria. ISBN 3–900051–07–0. Available at http://www.R-project.org.Google Scholar
Sánchez, FJ, Manzanares, M, de Andrés, EF, Tenorio, JL and Ayerbe, L (2001) Residual transpiration rate, epicuticular wax load and leaf colour of pea plants in drought conditions. Influence on harvest index and canopy temperature. European Journal of Agronomy 15: 57–70.Google Scholar
Sharma, P, Kothari, SL, Rathore, MS and Gour, VS (2018) Properties, variations, roles, and potential applications of epicuticular wax: a review. Turkish Journal of Botany 42: 135–149.Google Scholar
Wang, H and Clarke, JM (1993) Genotypic, intraplant, and environmental variation in stomatal frequency and size in wheat. Canadian Journal of Plant Science 73: 671–678.Google Scholar
Xue, D, Zhang, X, Lu, X, Chen, G and Chen, Z-H (2017) Molecular and evolutionary mechanisms of cuticular wax for plant drought tolerance. Frontiers in Plant Science 8: 621.Google Scholar
Zhang, JY, Broeckling, CD, Blancaflor, EB, Sledge, MK, Sumner, LW and Wang, ZY (2005) Overexpression of WXP1, a putative Medicago truncatula AP2 domain-containing transcription factor gene, increases cuticular wax accumulation and enhances drought tolerance in transgenic alfalfa (Medicago sativa). The Plant Journal 42: 689–707.Google Scholar
Supplementary material: File

Khazaei et al. supplementary material

Figure S1

Download Khazaei et al. supplementary material(File)
File 88.1 KB
Supplementary material: File

Khazaei et al. supplementary material

Tables S1-S3

Download Khazaei et al. supplementary material(File)
File 47.5 KB