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Genetic outcomes from a farmer-assisted landrace selection programme to develop a synthetic variety of broccoli

Published online by Cambridge University Press:  09 January 2014

Simona Ciancaleoni ,
Lorenzo Raggi  and
Valeria Negri
Simona Ciancaleoni
Affiliation:
Dipartimento di Biologia Applicata (DBA), University of Perugia, Borgo XX Giugno 74, Perugia06121, Italy
Lorenzo Raggi
Affiliation:
Dipartimento di Biologia Applicata (DBA), University of Perugia, Borgo XX Giugno 74, Perugia06121, Italy
Valeria Negri*
Affiliation:
Dipartimento di Biologia Applicata (DBA), University of Perugia, Borgo XX Giugno 74, Perugia06121, Italy
*
* Corresponding author. Fax: +390755856224. E-mail: [email protected]
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Abstract

To develop synthetic varieties (Syn) of broccoli for organic agriculture, we initiated a breeding programme from a landrace (LR). A Syn was obtained through a farmer-assisted selection programme that mirrors the original LR. The diversity level of the Syn was assessed using 14 putatively neutral microsatellite markers (simple sequence repeats (SSR)) and seven SSR related to genes involved in flowering control. Four commercial F1 hybrids were also assessed. Despite the strict selection procedure applied by the farmer to reproduce the LR annually and to obtain the Syn, the detected diversity level was high and similar to that of non-selected LRs. The possible reasons for these genetic outcomes (i.e. SSR position in the genome and farmer selection methods) are discussed here.

Keywords

Brassica oleracea L.dynamic on-farm conservationfarmer-assisted breedinggenetic diversity
Type
Short Communication
Information
Plant Genetic Resources , Volume 12 , Issue 3 , December 2014 , pp. 349 - 352
Copyright
Copyright © NIAB 2014 

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