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Genetic diversity assessment of the almond (Prunus dulcis (Mill.) D.A. Webb) traditional germplasm of Algarve, Portugal, using molecular markers

Published online by Cambridge University Press:  16 July 2014

Luis Cabrita ,
Elena Apostolova ,
Alcinda Neves ,
António Marreiros  and
José Leitão
Luis Cabrita
Affiliation:
Faculdade de Ciências e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139Faro, Portugal
Elena Apostolova
Affiliation:
Faculdade de Ciências e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139Faro, Portugal
Alcinda Neves
Affiliation:
Faculdade de Ciências e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139Faro, Portugal
António Marreiros
Affiliation:
Direcção Regional de Agricultura e Pescas do Algarve, Apartado 282, Patacão, 8001-904Faro, Portugal
José Leitão*
Affiliation:
Faculdade de Ciências e Tecnologia, Universidade do Algarve, Campus de Gambelas, 8005-139Faro, Portugal
*
* Corresponding author. E-mail: [email protected]
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Abstract

In this study, 123 almond (Prunus dulcis (Mill.) D.A. Webb) trees identified among traditional orchards in the Algarve region and 53 trees of the local field collection managed by the regional office of the Portuguese Ministry of Agriculture (DRAALG) were assessed using isozyme, inter-single sequence repeat and simple sequence repeat or microsatellite techniques for the evaluation of genetic diversity and genetic relatedness and identification of new accessions for the field collection. The isozyme analysis allowed the distribution of the 176 plants into 13 different classes of enzyme similarity, while the use of DNA markers increased the distribution of the analysed trees among 140 discriminating DNA patterns. Multiple cases of homonymy and synonymy were identified in the local germplasm. Some traditional varieties, such as Lourencinha, appeared to be relatively homogeneous, while other local denominations, e.g. Galamba, included diverse genotypes. Of the 13 commercial varieties analysed in this study, 11 assembled in one major cluster clearly differentiated from the majority of the local genotypes. These results reinforced the perception that the Algarve traditional germplasm constitutes an important repository of genetic diversity, eventually carrying alleles of high agricultural interest such as the recently identified Phomopsis resistance in the traditional variety Barrinho Grado.

Keywords

almondgermplasmisozymesISSRsmicrosatellites
Type
Research Article
Information
Plant Genetic Resources , Volume 12 , Issue S1: Special issue on the 3rd International Symposium on “Genomics of Plant Genetic Resources” , July 2014 , pp. S164 - S167
Copyright
Copyright © NIAB 2014 

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References

Arulsekar, S, Parfitt, DE and Kester, DE (1986) Comparison of isozyme variability in peach and almond cultivars. Journal of Heredity 77: 272–274.CrossRefGoogle Scholar
Arús, P, Olarte, C, Romero, M and Vargas, F (1994) Linkage analysis of ten isozyme genes in F1 segregating almond progenies. Journal of the American Society for Horticultural Science 119: 334–339.Google Scholar
Bartolozzi, F, Warburton, ML, Arulsekar, S and Gradziel, TM (1998) Genetic characterization and relatedness among California almond cultivars and breeding lines detected by randomly amplified polymorphic DNA (RAPD) analysis. Journal of the American Society for Horticultural Science 123: 381–387.CrossRefGoogle Scholar
Cabrita, L, Neves, A and Leitão, J (2004) Evaluation of resistance to Phomopsis amygdali in almond. Acta Horticulturae (ISHS) 663: 235–238.Google Scholar
Cao, MQ, Jiang, YQ, Ma, R, Ma, Y, Xie, H and Xu, Y (2005) Molecular analysis of almond germplasm in China. In: Oliveira, MM and Cordeiro, V (eds) XIII GREMPA Meeting on Almonds and Pistachios. Zaragoza, Spain: CIHEAM. Options Méditerranéennes: Série A 63, pp. 281–290.Google Scholar
Cipriani, G, Lot, G, Huang, W-G, Marrazzo, MT and Peterlunger, E (1999) AC/GT and AG/CT microsatellite repeats in peach [Prunus persica (L.) Batsch]: isolation, characterisation and cross-species amplification in Prunus . Theoretical and Applied Genetics 99: 65–72.Google Scholar
Dangl, GS, Yang, J, Golino, DA and Gradziel, T (2009) A practical method for almond cultivar identification and parental analysis using simple sequence repeat markers. Euphytica 168: 41–48.CrossRefGoogle Scholar
Elisiário, PJ, Neto, MC, Cabrita, LF and Leitão, JM (1998) Isozyme and RAPDs characterization of a collection of Fig (Ficus carica L.) traditional varieties. Acta Horticulturae (ISHS) 480: 149–154.CrossRefGoogle Scholar
Elisiário, PJ, Santos, GG, Guerreiro, AR, Ollitrault, P, Luro, F and Leitão, JM (1999) Isozyme analysis revealed that the Portuguese mandarin ‘Carvalhais’ originated as a single clone. Scientia Horticulturae 82: 145–152.CrossRefGoogle Scholar
Farinhó, M, Coelho, P, Carlier, J, Svetleva, D, Monteiro, A and Leitão, J (2004) Mapping of a locus for adult plant resistance to downy mildew in broccoli (Brassica oleracea convar. Italica). Theoretical and Applied Genetics 109: 1392–1398.Google Scholar
Goulão, L, Cabrita, L, Oliveira, CM and Leitão, JM (2001) Comparing RAPD and AFLPTM analysis in discrimination and estimation of genetic similarities among apple (Malus domestica Borkh.) cultivars. Euphytica 119: 259–270.Google Scholar
Joobeur, T, Periam, N, de Vicente, MC, King, G and Arús, P (2000) Development of a second generation linkage map for almond using RAPD and SSR markers. Genome 43: 649–655.Google Scholar
Martins, M, Farinha, A, Ferreira, E, Cordeiro, V, Monteiro, A, Tenreiro, R and Oliveira, M (2001) Molecular analysis of the genetic variability of Portuguese almond collections. Acta Horticulturae (ISHS) 546: 449–452.CrossRefGoogle Scholar
Martins, M, Tenreiro, R and Oliveira, MM (2003) Molecular diversity among Portuguese almond cultivars assessed by RAPD and ISSR markers. Plant Cell Reports 22: 71–78.Google Scholar
Monte-Corvo, L, Cabrita, L, Oliveira, C and Leitão, J (2000) Assessment of genetic relationships among Pyrus species and cultivars using AFLP and RAPD markers. Genetic Resources and Crop Evolution 47: 257–265.Google Scholar
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