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Genetic characterization of some wild grape populations (Vitis vinifera subsp. sylvestris) of Zagros mountains (Iran) to indentify a conservation strategy

Published online by Cambridge University Press:  24 April 2014

Hamed Doulati-Baneh ,
Seyed Abolghasem Mohammadi ,
Massimo Labra ,
Fabrizio De Mattia ,
Ilaria Bruni ,
Valerio Mezzasalma  and
Rahim Abdollahi
Hamed Doulati-Baneh
Affiliation:
Agricultural Research Center of West Azerbaijan, PO Box 365, Ourmia, Iran
Seyed Abolghasem Mohammadi
Affiliation:
Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz 51666, Iran
Massimo Labra*
Affiliation:
Department of Biosciences and Biotechnology, University of Milano-Bicocca, Piazza della Scienza 2, Milan I-20126, Italy
Fabrizio De Mattia
Affiliation:
Department of Biosciences and Biotechnology, University of Milano-Bicocca, Piazza della Scienza 2, Milan I-20126, Italy
Ilaria Bruni
Affiliation:
Department of Biosciences and Biotechnology, University of Milano-Bicocca, Piazza della Scienza 2, Milan I-20126, Italy
Valerio Mezzasalma
Affiliation:
Department of Biosciences and Biotechnology, University of Milano-Bicocca, Piazza della Scienza 2, Milan I-20126, Italy
Rahim Abdollahi
Affiliation:
Ms Horticulture, Oshnaviyeh Payame Noor University, West Azerbaijan Province, Tehran, Iran
*
*Corresponding author. E-mail: [email protected]
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Abstract

In this study, the genetic constitution of wild grape populations distributed in the Zagros Mountains in the north-west part of Iran was investigated to assess their risk of extinction and to identify the most suitable conservation strategy. A total of 65 wild grapevine samples from five different populations were analysed using 23 SSR loci. Consistent allelic richness was detected in all the analysed populations (from 4.01 to 5.35). The observed heterozygosity and expected heterozygosity were high with mean values of 0.67 and 0.69, respectively. A modest heterozygosity deficiency was detected only in the Piranshahr and Sardasht-Shalmash populations. The neighbour-joining dendrogram clustered the accessions into three groups corresponding to the geographical distribution of the populations. Molecular data combined with field evaluation revealed that an ex situ conservation approach is the most suitable strategy for preserving the genetic richness of Iranian wild grape populations with consistent rare alleles (about 20%). We propose in situ preservation only in the case of the Sardasht-Ghasmarash population to ensure that evolutionary dynamic forces continue to influence plant adaptation and survival in response to environmental alterations.

Keywords

genetic resourcesgermplasm collectionsmolecular markersplant conservationSSRswild grapevines
Type
Research Article
Information
Plant Genetic Resources , Volume 13 , Issue 1 , April 2015 , pp. 27 - 35
Copyright
Copyright © NIAB 2014 

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