Hostname: page-component-78c5997874-t5tsf Total loading time: 0 Render date: 2024-11-17T18:04:14.799Z Has data issue: false hasContentIssue false
  • English
  • Français

Flavonoid content in seeds of guar germplasm using HPLC

Published online by Cambridge University Press:  01 August 2007

M. L. Wang  and
J. B. Morris
M. L. Wang*
Affiliation:
USDA-ARS, Plant Genetic Resources Conservation Unit, 1109 Experiment Street, Griffin, GA 30223, USA
J. B. Morris
Affiliation:
USDA-ARS, Plant Genetic Resources Conservation Unit, 1109 Experiment Street, Griffin, GA 30223, USA
*
*Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Legume flavonoids have received considerable attention due to their beneficial effects on human health. Flavonoid content in the seeds of 36 germplasm accessions of guar [Cyamopsis tetragonoloba (L.) Taub.] was quantified by high-performance liquid chromatography (HPLC). On a per 100 g basis, the seed contained, on average, 1.114 mg (0–2.355) daidzein, 0.700 mg (0–1.685) genistein, 0.553 mg (0–1.250) quercetin and 14.460 mg (10.70–19.82) kaempferol. A significant positive correlation was observed between the content of daidzein and genistein; however, significant negative correlations existed between the content of kaempferol and daidzein and of kaempferol and genistein. Compared to soybean seeds, guar seeds contained very low amounts of daidzein and genistein, but a high amount of kaempferol. The information about the levels of flavonoids in guar seeds will be useful to breeding programmes for improving guar seed quality. Furthermore, the high kaempferol content of guar seeds may expand its nutraceutical and pharmaceutical utilization.

Keywords

flavonoid contentgermplasmguarhigh-performance liquid chromatography
Type
In Brief
Information
Plant Genetic Resources , Volume 5 , Issue 2 , August 2007 , pp. 96 - 99
Copyright
Copyright © NIAB 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Dhaubhadel, S, McGrarvey, BD, Williams, R and Gijzen, M (2003) Isoflavonoid biosynthesis and accumulation in developing soybean seeds. Plant Molecular Biology 53: 733–743.CrossRefGoogle ScholarPubMed
Hymowitz, T and Matlock, RS (1963) Guar in the United States. Oklahoma Agriculture Experiment Station Bulletin B 611: 1–34.Google Scholar
Koes, RE, Quattrocchio, F and Mol, JNM (1994) The flavonoid biosynthetic pathway in plants: function and evolution. BioEssays 16: 123–132.CrossRefGoogle Scholar
Morris, JB (2004) Legumes: nutraceutical and pharmaceutical uses. In: Goodman, RM (ed.) Encyclopedia of Plant and Crop Science. New York: Marcel Dekker, pp. 651–655.CrossRefGoogle Scholar
Whistler, RL and Hymowitz, T (1979) Speciation and cytogenetics. In: Whistler, RL and Hymowitz, T (eds) Guar: Agronomy, Production, Industrial Use, and Nutrition. West Lafayette, IN: Purdue University Press, pp. 16–28.Google Scholar