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Evaluation of genetic variations at glutenin loci in Korean wheat landraces using allele-specific DNA markers

Published online by Cambridge University Press:  30 October 2014

Jeong Hwan Ahn ,
Soo-Kyung Lee  and
Chul Soo Park
Jeong Hwan Ahn
Affiliation:
Department of Crop Science and Biotechnology, Chonbuk National University, Jeonju561-756, Korea
Soo-Kyung Lee
Affiliation:
National Academy of Agricultural Science, RDA, Suwon431-707, Korea
Chul Soo Park*
Affiliation:
Department of Crop Science and Biotechnology, Chonbuk National University, Jeonju561-756, Korea
*
* Corresponding author. E-mail: [email protected]
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Abstract

The allelic variations at glutenin loci could significantly affect the bread baking quality, and specific glutenin alleles might be closely associated with greater gluten strength, which, in turn, is related to superior bread baking quality. In this study, allelic variations at Glu-1, Glu-A3 and Glu-B3 loci were evaluated in 222 Korean wheat landraces using gene-specific polymerase chain reaction (PCR) markers. Ten alleles were identified at Glu-1 loci. Glu-A1c, Glu-B1b, and Glu-D1a or Glu-D1f alleles were predominantly found at the respective loci and their frequencies were 86.5, 87.8 and 96.9 %, respectively. Seven Korean wheat landraces carried the Glu-D1d allele, and only one Korean wheat landrace (IT173162) achieved 10 points for the Glu-1 score. Fifteen alleles were identified at Glu-A3 and Glu-B3 loci; Glu-A3c and Glu-B3d or Glu-B3i alleles were commonly found at the respective loci and their frequencies were 77.0, 33.3 and 37.8 %, respectively. Glu-B3 alleles exhibited the highest genetic diversity than other alleles, while Glu-B1 and Glu-A1 alleles exhibited the lowest genetic diversity than other alleles. Twenty Korean wheat landraces had the Glu-A3d and Glu-B3b, Glu-B3d, Glu-B3f, Glu-B3g or Glu-B3i alleles, which were correlated with superior bread baking quality. Among these wheat lines, two (IT59787 and IT236544) carried the Glu-D1d allele.

Keywords

Glu-1Glu-A3Glu-B3Korean wheatlandraces
Type
Short Communication
Information
Plant Genetic Resources , Volume 12 , Issue 3 , December 2014 , pp. 353 - 356
Copyright
Copyright © NIAB 2014 

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References

Branlard, G, Dardevet, M, Amiour, N and Igrejas, G (2003) Allelic diversity of HMW and LMW glutenin subunits and omega-gliadins in French bread wheat (Triticum aestivum L.). Genetic Resources and Crop Evolution 50: 669–679.Google Scholar
De Bustos, A, Rubio, P, Soler, C, García, P and Jouve, N (2001) Marker assisted selection to improve HMW-glutenins in wheat. Euphytica 119: 69–73.Google Scholar
D'Ovidio, R and Masci, S (2004) The low-molecular-weight glutenin subunits of wheat gluten. Journal of Cereal Science 39: 321–339.CrossRefGoogle Scholar
Eagles, HA, Hollamby, GJ, Gororo, NN and Eastwood, RF (2002) Estimation and utilization of effects from the analysis of unbalanced data from wheat breeding programs. Australian Journal of Agricultural Research 53: 367–377.Google Scholar
Gianibelli, MC, Larroque, OR, Mac-Ritchie, F and Wrigley, CW (2001) Biochemical, genetic and molecular characterization of wheat glutenin and its component subunits. Cereal Chemistry 78: 635–646.CrossRefGoogle Scholar
Goutam, U, Kukreja, S, Tiwari, R, Chaudhury, A, Gupta, RK, Dholakia, BB and Yadav, R (2013) Biotechnological approaches for grain quality improvement in wheat: present status and future possibilities. Australian Journal of Crop Science 7: 469–483.Google Scholar
Lafiandra, D, Tucci, GF, Pavoni, A, Turchetta, T and Margiotta, B (1997) PCR analysis of x- and y-type genes present at the complex Glu-A1 locus in durum and bread wheat. Theoretical and Applied Genetics 94: 235–240.Google Scholar
Lei, ZS, Gale, KR, He, ZH, Gianibelli, C, Larroque, O, Xia, XC, Butow, BJ and Ma, W (2006) Y-type gene specific markers for enhanced discrimination of high-molecular weight glutenin alleles at the Glu-B1 locus in hexaploid wheat. Journal of Cereal Science 43: 94–101.CrossRefGoogle Scholar
Lerner, SE, Kolman, MA and Rogers, WJ (2009) Quality and endosperm storage protein variation in Argentinean grown bread wheat. I. Allelic diversity and discrimination between cultivars. Journal of Cereal Science 49: 337–345.Google Scholar
Li, Y, Huang, C, Sui, X, Fan, Q, Li, G and Chu, X (2009) Genetic variation of wheat glutenin subunits between landraces and varieties and their contributions to wheat quality improvement in China. Euphytica 169: 159–168.Google Scholar
Liu, SX, Chao, SM and Anderson, JA (2008) New DNA markers for high molecular weight glutenin subunits in wheat. Theoretical and Applied Genetics 118: 177–183.Google Scholar
Ma, W, Zhang, W and Gale, KR (2003) Multiplex-PCR typing of high molecular weight glutenin alleles in wheat. Euphytica 134: 51–60.Google Scholar
Nakamura, H, Inazu, A and Hirano, H (1999) Allelic variation in high-molecular-weight glutenin subunit loci of Glu-1 in Japanese common wheats. Euphytica 106: 131–138.Google Scholar
Park, CS, Kang, C-S, Jeung, J-U and Woo, S-H (2011) Influence of allelic variations in glutenin on the quality of pan bread and white salted noodles made from Korean wheat cultivars. Euphytica 180: 235–250.Google Scholar
Payne, PI (1987) Genetics of wheat storage proteins and the effects of allelic variation on bread-making quality. Annual Review of Plant Physiology 38: 141–153.CrossRefGoogle Scholar
Payne, PI and Lawrence, GJ (1983) Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1 and Glu-D1 which code for high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Research Communication 11: 29–35.Google Scholar
Payne, PI, Nightingale, MA, Krattiger, AF and Holt, M (1987) The relationship between HMW glutenin subunit composition and the bread-making quality of British-grown wheat varieties. Journal of the Science of Food and Agriculture 40: 51–65.CrossRefGoogle Scholar
Peakall, R and Smouse, P (2006) GenALEx 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6: 288–295.Google Scholar
Peña, RJ, Gonzalez-Santoyo, H and Cervantes, F (2004) Relationship between Glu-D1/Glu-B3 allelic combinations and bread-making quality-related parameters commonly used in wheat breeding. In: Lafiandra, D, Masci, S and D'Ovidio, R (eds) The Gluten Proteins. UK: Royal Society of Chemistry, pp. 156–159.Google Scholar
Shan, X, Clayshulte, SR, Haley, SD and Byrne, PF (2007) Variation for glutenin and waxy alleles in the US hard winter wheat germplasm. Journal of Cereal Science 45: 199–208.Google Scholar
Shewry, PI, Halford, NG and Tatham, AS (1992) High molecular weight subunits of wheat glutenin. Journal of Cereal Science 15: 105–120.CrossRefGoogle Scholar
Singh, NK and Shepherd, KW (1988) Linkage mapping of genes controlling endosperm storage proteins in wheat. I. Genes on the short arms of group 1 chromosomes. Theoretical and Applied Genetics 75: 628–641.Google Scholar
Takata, K, Nishio, Z, Iriki, N, Tabiki, T, Funatsuki, W and Yamauchi, H (2005) Comparison of quality characteristics of waxy wheat using near-isogenic line. Breeding Science 55: 87–92.CrossRefGoogle Scholar
Terasawa, Y, Takata, K, Hirano, H, Kato, K, Kawahara, T, Sasakuma, T and Sasanuma, T (2011) Genetic variation of high-molecular-weight glutenin subunit composition in Asian wheat. Genetic Resources and Crop Evolution 58: 283–289.CrossRefGoogle Scholar
Wang, L, Zhao, XL, He, ZH, Ma, W, Appels, R, Peña, RJ and Xia, XC (2009) Characterization of low-molecular-weight glutenin subunit Glu-B3 genes and development of STS markers in common wheat (Triticum aestivum L.). Theoretical and Applied Genetics 118: 525–539.Google Scholar
Wang, L, Li, G, Peña, RJ, Xia, X and He, Z (2010) Development of STS markers and establishment of multiplex PCR for Glu-A3 alleles in common wheat (Triticum aestivum L.). Journal of Cereal Science 51: 305–312.CrossRefGoogle Scholar
Zhang, X, Jin, H, Zhang, Y, Liu, D, Li, G, Xia, X, He, Z and Zhang, A (2012) Composition and functional analysis of low-molecular-weight glutenin alleles with Aroona near-isogenic lines of bread. BMC Plant Biology 12: 243.Google Scholar
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