Hostname: page-component-586b7cd67f-t7fkt Total loading time: 0 Render date: 2024-11-22T04:10:13.377Z Has data issue: false hasContentIssue false
  • English
  • Français

A DNA fingerprinting-based taxonomic allocation of Kamut wheat

Published online by Cambridge University Press:  08 March 2007

Elena K. Khlestkina ,
Marion S. Röder ,
Heinrich Grausgruber  and
Andreas Börner
Elena K. Khlestkina
Affiliation:
Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstr. 3, D-06466 Gatersleben, Germany Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences, Lavrentyeva ave. 10, Novosibirsk, 630090, Russia
Marion S. Röder
Affiliation:
Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstr. 3, D-06466 Gatersleben, Germany
Heinrich Grausgruber
Affiliation:
BOKU—University of Natural Resources and Applied Life Sciences, Institute of Agronomy and Plant Breeding, Gregor Mendel Str. 33, A-1180 Vienna, Austria
Andreas Börner*
Affiliation:
Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstr. 3, D-06466 Gatersleben, Germany
*
* E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

Kamut wheat, said to have been derived from seed found in the Egyptian pyramids, appeared on the market about 25 years ago. We have investigated its taxonomic placement using microsatellite genotyping. In all, 89 accessions of 13 tetraploid wheat species, along with samples of Kamut wheat, were genotyped using two A and B genome wheat microsatellite markers per chromosome, generating 453 alleles (8–33 alleles per locus), and a mean allelic polymorphic information content (PIC) of 0.80. A diversity analysis showed that nine major accession groups could be defined, and these were inconsistent with formal taxonomic classifications of about 10% of the material. Most of these misclassifications are due to either species introgression or seed admixture. Some accessions appear to be duplicates. The Kamut wheats grouped together in a cluster containing three accessions of Triticum polonicum and three of T. durum, originating from Turkey, Iraq, Iran and Israel. We suggest that Kamut perhaps derived from a natural hybrid between T. durum and T. polonicum, which occurred in the Fertile Crescent.

Keywords

Kamut wheatmicrosatellite markerstetraploid wheat speciesTriticum ssp
Type
Research Article
Information
Plant Genetic Resources , Volume 4 , Issue 3 , December 2006 , pp. 172 - 180
Copyright
Copyright © NIAB 2006

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alamerew, S, Chebotar, S, Huang, XQ, Röder, MS and Börner, A (2004) Genetic diversity in Ethiopian hexaploid and tetraploid wheat germplasm assessed by microsatellite markers. Genetic Resources and Crop Evolution 51: 559–567.CrossRefGoogle Scholar
Anderson, JA, Churchill, GA, Antrique, JE, Tanksley, SD and Sorrels, ME (1993) Optimising parental selection for genetic linkage maps. Genome 36: 181–188.CrossRefGoogle Scholar
Ben, Amer IM, Börner, A and Röder, MS (2001) Detection of genetic diversity in Libyan wheat genotypes using wheat microsatellite markers. Genetic Resources and Crop Evolution 48: 579–585.Google Scholar
Chebotar, SV and Sivolap, YM (2001) Differentiation, identification and characterization of Triticum aestivum L. varieties from Ukrainian breeding programs by using STMS analysis. Cytology and Genetics 35: 18–27.(in Russian)Google Scholar
Dice, LR (1945) Measures of the amount of ecologic association between species. Ecology 26: 297–302.CrossRefGoogle Scholar
Donini, P, Stephenson, P, Bryan, GJ and Koebner, RMD (1998) The potential of microsatellites for high throughput genetic diversity assessment in wheat and barley. Genetic Resources and Crop Evolution 45: 415–421.CrossRefGoogle Scholar
Dorofeev, VF, Filatenko, AA, Migushova, EF, Udachin, RA andJakubziner, MM (1979) Flora of Cultivated Plants. Leningrad: Kolos, Leningrad Branch (in Russian).Google Scholar
Fahima, T, Röder, MSGrama, A and Nevo, E (1998) Microsatellite DNA polymorphism divergence in Triticum dicoccoides accessions highly resistant to yellow rust. Theoretical and Applied Genetics 96: 187–195.CrossRefGoogle Scholar
Hammer, K, Filatenko, AA and Korzun, V (2000) Microsatellite markers–a new tool for distinguishing diploid wheat. Genetic Resources and Crop Evolution 47: 497–505.CrossRefGoogle Scholar
Huang, XQ, Börner, A, Röder, MS and Ganal, MW (2002) Assessing genetic diversity of wheat ( Triticum aestivum L.) germplasm using microsatellite markers. Theoretical and Applied Genetics 105: 699–707.CrossRefGoogle ScholarPubMed
Kasarda, DD (2001) Grains in relation to celiac disease. Cereal Foods World 46: 209–210.Google Scholar
Khlestkina, EK, Huang, XQ, Quenum, FJBChebotar, S, Röder, MS and Börner, A (2004 a) Genetic diversity in cultivated plants–loss or stability?. Theoretical and Applied Genetics 108: 1466–1472.CrossRefGoogle ScholarPubMed
Khlestkina, EK, Röder, MS, Efremova, TT, Börner, A and Shumny, VK (2004b) The genetic diversity of old and modern Siberian varieties of common spring wheat determined by microsatellite markers. Plant Breeding 123: 122–127.CrossRefGoogle Scholar
Nei, M (1973) Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of the USA 70: 3321–3323.CrossRefGoogle ScholarPubMed
Plaschke, J, Ganal, MW and Röder, MS (1995) Detection of genetic diversity in closely related bread wheat using microsatellite markers. Theoretical and Applied Genetics 91: 1001–1007.CrossRefGoogle ScholarPubMed
Quinn, RM (1999) Kamut ® ancient grain, new cereal. in Janick, J (ed.) Perspectives on New Crops and New Uses. Alexandria, VA: ASHS Press, pp 182–183.Google Scholar
Röder, MS, Plaschke, J, König, SU, Börner, A, Sorrells, ME, Tanksley, SD and Ganal, MW (1995) Abundance, variability and chromosomal location of microsatellites in wheat. Molecular and General Genetics 246: 327–333.CrossRefGoogle ScholarPubMed
Röder, MS, Korzun, V, Wendehake, K, Plaschke, J, Tixier, M-H, Leroy, P and Ganal, MW (1998) A microsatellite map of wheat. Genetics 149: 2007–2023.CrossRefGoogle ScholarPubMed
Röder, MS, Wendehake, K, Korzun, V, Bredemeijer, G, Laborie, D, Bertrand, L, Isaak, P, Rendell, S, Jackson, J, Cooke, RJ, Vosman, B and Ganal, MW (2002) Construction and analysis of a microsatellite-based database of European wheat varieties. Theoretical and Applied Genetics 106: 67–73.CrossRefGoogle ScholarPubMed
Rohlf, FJ (1998) NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System, version 2.0. New York: Applied Biostatistics.Google Scholar
Simonato, B, Pasini, G, Giannattasio, M and Curioni, A (2002) Allergenic potential of Kamut wheat. Allergy 57: 653–654.Google ScholarPubMed
Stallknecht, GF, Gilbertson, KM and Ranney, JE (1996) Alternative wheat cereals as food grains: Einkorn, Emmer, Spelt, Kamut, and Triticale. In: Janick, J (ed.) Progress in New Crops Alexandria, VA: ASHS Press. 156–170.Google Scholar