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Development of HP-TLC method for rapid quantification of sugars, catechins, phenolic acids and saponins to assess Yam (Dioscorea spp.) tuber flour quality

Published online by Cambridge University Press:  18 October 2018

V. Lebot ,
R. Malapa  and
T. Molisalé
V. Lebot*
Affiliation:
CIRAD, UMR AGAP, PO Box 946, Port-Vila, Vanuatu
R. Malapa
Affiliation:
Vanuatu Agricultural Research and Technical Centre, Santo PO Box 231, Vanuatu
T. Molisalé
Affiliation:
Department of Agriculture and Rural Development (DARD), Ministry of Agriculture, PMB Santo, Vanuatu
*
*Corresponding author. E-mail: [email protected]
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Abstract

Eight yam species (Dioscorea spp.) represented by 522 accessions (landraces and hybrids) were analysed for the individual sugars, catechins, phenolic acids and saponins contents of their tuber flours. Maltose, sucrose, glucose and fructose were quantified. Reducing sugars mean values were highly variable within species and ranged from 0.16%dry weight (DW) (SD ± 0.12) in D. dumetorum to 3.15%DW (±2.49) in D. esculenta. Maltose was detected only in D. esculenta. Chlorogenic acid, gallic acid and other phenolic acids ranged from 4.33 mg/g DW in D. bulbifera to 4.87 mg/g in D. alata and 9.55 mg/g in D. nummularia but were not detected in other species. Catechins (epicatechin and catechin) were highest in D. bulbifera bulbils (25.18 mg/g) and tubers (6.96 mg/g), and lowest in D. esculenta (0.32 mg/g). Their content is significantly correlated with dark flour colour and they most likely contribute to the oxidation of tuber flesh. Saponins (dioscin and gracillin) were quantified in only two species: D. cayenensis (5.94 mg/g, SD ± 3.78) and D. esculenta (3.74 mg/g, SD ± 3.72). Varietal selection may tend to reduce sugars levels and increase secondary metabolites with bioactive properties. HP-TLC is a suitable technique for the rapid quantification of compounds related to yam tuber flour quality.

Keywords

catechindioscinDioscoreaepicatechingracillintuber oxidation
Type
Research Article
Information
Plant Genetic Resources , Volume 17 , Issue 1 , February 2019 , pp. 62 - 72
Copyright
Copyright © NIAB 2018 

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Footnotes

†

Deceased.

References

Agatonovic-Kustrin, S, Milojkovic-Opsenica, D, Morton, DW and Ristivojevic, P (2017) Chemometric characterization of wines according to their HPTLC fingerprints. European Food Research and Technology 243: 659–667.Google Scholar
Akissoe, N, Mestres, C, Hounhouigan, J and Nago, M (2005) Biochemical origin of browning during the processing of fresh yam (Dioscorea spp.) into dried product. Journal of Agricultural and Food Chemistry 53: 2552–2557.Google Scholar
Asiedu, R and Sartie, A (2010) Crops that feed the world. Yams. Yams for income and food security. Food Security 2: 305–315.Google Scholar
Aumsuwan, P, Khan, S, Khan, IA, Ali, Z, Avula, B, Walker, LA, Shariat-Madar, Z, Helferich, WG, Katzenellenbogen, BS and Dasmahapatra, AK (2016) The anticancer potential of steroidal saponin, dioscin, isolated from wild yam (Dioscorea villosa) root extract in invasive human breast cancer cell line MDA-MB-231 in vitro. Archives of Biochemistry and Biophysics 591: 98–110.Google Scholar
Baah, FD, Maziya-Dixon, B, Asiedu, R, Oduro, I and Ellis, WO (2009) Physicochemical and pasting characterisation of water yam (Dioscorea spp.) and relationship with eating quality of pounded yam. Journal of Food Agriculture and Environment 7: 107–112.Google Scholar
Bai, Y, Zhang, L, Jin, W, Wei, M, Zhou, P, Zheng, G, Niu, L, Nie, L, Zhang, Y, Wang, H and Yu, L (2015) In situ high valued utilization and transformation of sugars from Dioscorea zingiberensis C.H. Wright for clean production of diosgenin. Bioresource Technology 96: 642–647.Google Scholar
Biedermann-Brem, S, Noti, A, Grob, K, Imhof, D, Bazzocco, D and Pfefferle, A (2003) How much reducing sugar may potatoes contain to avoid excessive acrylamide formation during roasting and baking? European Food Research and Technology 217: 369–373.Google Scholar
Champagne, A, Bernillon, S, Moing, A, Rolin, D, Legendre, L and Lebot, V (2010) Carotenoid profiling of tropical root crop chemotypes from Vanuatu, South Pacific. Journal of Food Composition and Analysis 23: 763–771.Google Scholar
Champagne, A, Bernillon, S, Moing, A, Rolin, D, Legendre, L and Lebot, V (2011) Diversity of anthocyanins and other phenolic compounds among tropical root crops from Vanuatu, South Pacific. Journal of Food Composition and Analysis 24: 315–325.Google Scholar
Chandrasekara, A and Kumar, TJ (2016) Roots and tuber crops as functional foods: a review on phytochemical constituents and their potential health benefits. International Journal of Food Science 2016: Article ID 3631647, 15 p. doi: dx.doi.org/10.1155/2016/3631647.Google Scholar
Cretu, G, Morlock, G, Raluca-Miron, A and Nechifor, AC (2013) A high-performance thin layer chromatographic method for chlorogenic acid and hyperoside determination from berry extracts. Romanian Biotechnological Letters 18: 8657–8665.Google Scholar
Deshpande, HA and Bhalsing, SR (2014) Plant derived novel biomedicinal: diosgenin. International Journal of Pharmacy and Phytochemistry Research 6: 780–784.Google Scholar
Ezeocha, VC and Ojimelukwe, PC (2012) The impact of cooking on the proximate composition and antinutritional factors of water yam (Dioscorea alata). Journal of Stored Products and Post Harvest Research 3: 172–176.Google Scholar
FAOSTAT (2017) http://www.fao.org (accessed 18 August 2017).Google Scholar
Fu, YC, Ferng, LHA and Huang, PY (2006) Quantitative analysis of allantoin and allantoic acid in yam tuber, mucilage, skin and bulbil of the Dioscorea species. Food Chemistry 94: 541–549.Google Scholar
Go, HK, Rahman, M, Kim, GB, Na, CS, Song, CH, Kim, JS, Kim, SJ and Kang, HS (2015) Antidiabetic effects of yam (Dioscorea batatas) and its active constituent, allantoin, in a rat model of streptozotocin-induced diabetes. Nutrition 7: 8532–8544.Google Scholar
Govaerts, R, Wilkin, P and Saunders, RMK (2007) World Checklist of Dioscoreales. Yams and Their Allies. Royal Botanic Gardens, Kew, UK: Kew Publishing, 65p.Google Scholar
Graham-Acquaah, S, Ayernor, GS, Bediako-Amoa, B, Saalia, FK and Afoakwa, EO (2014) Spatial distribution of total phenolic content, enzymatic activities and browning in white yam (Dioscorea rotundata) tubers. Journal of Food Science and Technology 51: 2833–2838.Google Scholar
Güçlü-Üstündagḡ, Ö and Mazza, G (2007) Saponins: properties, applications and processing. Critical Review in Food Science and Nutrition 47: 231–258.Google Scholar
Jesus, M, Martins, APJ, Gallardo, E and Silvestre, S (2016) Diosgenin: recent highlights on pharmacology and analytical methodology. Journal of Analytical Methods in Chemistry 2016, Article ID 4156293, 16 p. doi: doi.org/10.1155/2016/4156293.Google Scholar
Kwon, HJ, Choi, SH, Yoo, CS, Choi, HY, Lee, SE and Park, YD (2013) Development of an analytical method for yam saponins using HPLC with pulsed amperometric detection at different column temperatures. Journal of Separation Science 36: 690–698.Google Scholar
Kwon, YK, Jie, EY, Sartie, A, Kim, DJ, Liu, JR, Min, BW and Kim, SW (2015) Rapid metabolomics discrimination and prediction of dioscin content from African yam tubers using Fourier transform-infrared spectroscopy combined with multivariate analysis. Food Chemistry 166, 389–396.Google Scholar
Lebot, V and Malapa, R (2012) Application of near infrared reflectance spectroscopy to the evaluation of yam (Dioscorea alata) germplasm and breeding lines. Journal of the Science of Food and Agriculture 93: 1788–1797.Google Scholar
Liu, Y, Li, H, Fan, Y, Man, S, Liu, Z, Gao, W and Wang, T (2016) Antioxidant and antitumor activities of the extracts from Chinese yam (Dioscorea opposite Thunb) flesh and peel and the effective compounds. Journal of Food Science 81: 1553–1564.Google Scholar
Manjunatha, SS, Ravi, N, Negi, PS, Raju, PS and Bawa, AS (2014) Kinetics of moisture loss and oil uptake during deep fat frying of Gethi (Dioscorea kamoonensis Kunth) strips. Journal of Food Science and Technology 51: 3061–3071.Google Scholar
Morlock, GE and Gulnar, S (2012) Comparison of two orthogonal liquid chromatographic methods for quantification of sugars in food. Journal of Liquid Chromatography and Related Technologies 34: 902–919.Google Scholar
Muttucumaru, N, Powers, SJ, Elmor, JS, Dodson, A, Briddon, A, Mottram, DS and Halford, NG (2017) Acrylamide-forming potential of potatoes grown at different locations, and the ratio of free asparagine to reducing sugars at which free asparagine becomes a limiting factor for acrylamide formation. Food Chemistry 220: 76–86.Google Scholar
Price, EJ, Wilkin, P, Sarasan, V and Fraser, PD (2016) Metabolite profiling of Dioscorea (yam) species reveals underutilized biodiversity and renewable sources for high-value compounds. Nature Scientific Reports 6, 29316.Google Scholar
Price, EJ, Bhattacharjee, R, Lopez-Montes, A and Fraser, PD (2018) Carotenoid profiling of yams: clarity, comparisons and diversity. Food Chemistry 259: 130–138.Google Scholar
Sautour, M, Mittaine-Offer, AC and Lacaille-Dubois, MA (2007) The Dioscorea genus: a review of bioactive steroid saponins. Journal of Natural Medicine 61, 91–101.Google Scholar
Skinner, DZ, Bauchan, GR, Auricht, G and Hughes, S (1999) A method for the efficient management and utilization of large germplasm collections. Crop Science 39: 1237–1242.Google Scholar
Spartzak, B, Krauze-Baranowska, M and Poblocka-Olech, L (2008) HPTLC determination of catechins in in-vitro cultures of two species of the genus Phyllanthus. Journal of Planar Chromatography 21: 103–108.Google Scholar
Wireko-Manu, FD, Ellis, WO, Oduro, I, Asiedu, R and Maziya-Dixon, B (2011) Physicochemical and pasting characteristics of water yam (D. alata) in comparison with pona (D. rotundata) from Ghana. European Journal of Food Research and Review 1: 149–158.Google Scholar
Wu, WH, Liu, LY, Chung, CJ, Jou, HJ and Wang, TA (2005) Estrogenic effect of yam ingestion in healthy postmenopausal women. Journal of the American College of Nutrition 24: 235–243.Google Scholar
Zhang, Z, Gao, W, Wang, R and Huang, W (2014) Changes in main nutrients and medicinal composition of Chinese yam (Dioscorea opposita) tubers during storage. Journal of Food Science and Technology 51: 2435–2543.Google Scholar
Zhang, Y, Xu, Y, Qi, Y, Xu, L, Song, S, Yin, L, Tao, X, Zhen, Y, Han, X, Ma, X, Liu, K and Peng, J (2017) Protective effects of dioscin against doxorubicin-induced nephrotoxicity via adjusting FXR-mediated oxidative stress and inflammation. Toxicology 378: 53–64.Google Scholar
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