Hostname: page-component-cd9895bd7-gxg78 Total loading time: 0 Render date: 2024-12-24T03:10:36.706Z Has data issue: false hasContentIssue false
  • English
  • Français

Development and characterization of polymorphic microsatellite markers for Spathoglottis plicata (Orchidaceae)

Published online by Cambridge University Press:  03 August 2018

Chiuan-Yu Li ,
Chi-Chun Huang ,
Chaur-Tzuhn Chen  and
Kuo-Hsiang Hung
Chiuan-Yu Li
Affiliation:
Taiwan Endemic Species Research Institute, Nantou, Taiwan Graduate Institute of Bioresources, Pingtung University of Science and Technology, Pingtung, Taiwan
Chi-Chun Huang
Affiliation:
Kinmen National Park, Kinmen, Taiwan
Chaur-Tzuhn Chen
Affiliation:
Department of Forestry, Pingtung University of Science and Technology, Pingtung, Taiwan
Kuo-Hsiang Hung*
Affiliation:
Graduate Institute of Bioresources, Pingtung University of Science and Technology, Pingtung, Taiwan
*
*Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

We developed novel and polymorphic microsatellite primers for Spathoglottis plicata, a tropical and subtropical terrestrial orchid, to investigate the genetic patterns and population structure among wild populations, and also to identify the varieties and hybrids of S. plicata in horticultural industry. The 12 novel microsatellites from S. plicata were developed by using polymerase chain reaction (PCR)-based isolation of microsatellite arrays. These markers that were successfully PCR amplified exhibited polymorphisms in S. plicata. The number of alleles, observed heterozygosity, expected heterozygosity and polymorphism information content values across loci ranged from 2.000 to 8.000, 0.000 to 0.756, 0.208 to 0.813 and 0.405 to 0.805 in total populations, respectively. The newly developed microsatellite markers exhibited variation in S. plicata. These markers can be used as a tool to further investigate the genetic diversity, conservation genetics and variety/hybrid identification of S. plicata.

Keywords

conservation geneticsgenetic diversitymicrosatellitepolymorphismSpathoglottis plicata
Type
Short Communication
Information
Plant Genetic Resources , Volume 16 , Issue 6 , December 2018 , pp. 572 - 575
Copyright
Copyright © NIAB 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

†

These authors contributed equally to this work.

References

Backhouse, G (2007) Are our orchids safe down under? A national assessment of threatened orchids in Australia. Lankesteriana 7: 28–43.Google Scholar
Benson, G (1999) Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acids Research 27: 573–580.Google Scholar
Cronquist, A (1981) An Integrated System of Classification of Flowering Plants. Ithaca: Columbia University Press.Google Scholar
Doyle, JJ and Doyle, JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11–15.Google Scholar
Hung, KH, Lin, CY, Huang, CC, Hwang, CC, Hsu, TW, Kuo, YL, Wang, WK, Hung, CY and Chiang, TY (2012) Isolation and characterization of microsatellite loci from Pinus massoniana (Pinaceae). Botanical Studies 53: 191–196.Google Scholar
Lavarack, PS (1984) Spathoglottis plicata: a mystery solved. Orchadian 7: 160.Google Scholar
Liu, K and Muse, SV (2005) Powermarker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21: 2128–2129.Google Scholar
Lunt, DH, Hutchinson, WF and Carvalho, GR (1999) An efficient method for PCR-based isolation of microsatellite arrays (PIMA). Molecular Ecology 8: 891–894.Google Scholar
Peakall, R and Smouse, PE (2012) Genalex 6.5: genetic analysis in Excel. Population genetic software for teaching and research-an update. Bioinformatics 28: 2537–2539.Google Scholar
Raymond, M and Rousset, F (1995) GENEPOP (version-1.2): population genetics software for exact tests and ecumenicism. Journal of Heredity 86: 248–249.Google Scholar
Teoh, ES (2005) Orchids of Asia, 3rd ed. Marshall Cavendish, Singapore.Google Scholar
Untergrasser, A, Cutcutache, I, Koressaar, T, Ye, J, Faircloth, BC, Remm, M and Rozen, SG (2012) Primer3 – new capabilities and interfaces. Nucleic Acids Research 40: e115.Google Scholar
Wang, JC, Chiou, WL and Chang, HM (2012) A Preliminary Red List of Taiwanese Vascular Plants. Nantou: Endemic Species Research Institute (In Chinese).Google Scholar