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Determining genetic combining ability, heterotic potential and gene action for yield contributing traits and Yellow Vein Mosaic Virus (YVMV) resistance in Okra (Abelmoschus esculentus (L.) Monech.)

Published online by Cambridge University Press:  27 October 2020

Anjan Das Open the ORCID record for Anjan Das [Opens in a new window] ,
Ramesh Kumar Yadav ,
Harshawardhan Choudhary ,
Saurabh Singh Open the ORCID record for Saurabh Singh [Opens in a new window] ,
Yogesh P. Khade  and
Rahul Chandel
Anjan Das*
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India
Ramesh Kumar Yadav
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India
Harshawardhan Choudhary
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India
Saurabh Singh
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India
Yogesh P. Khade
Affiliation:
ICAR-Directorate of Onion and Garlic Research, Rajgurunagar, Pune-410505, India
Rahul Chandel
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi-110012, India
*
*Corresponding author. E-mail: [email protected]
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Abstract

Breeding for resistance to biotic stress and higher yield is a continuous process. Thus, the identification of desirable parents with good combining ability and nature of gene action for the target trait is of utmost importance. Hence, in this present investigation, 10 lines and three testers of Okra were crossed in line × tester mating design to generate 30 testcross progenies and their evaluation along with parents and check in a randomized complete block design with three replications. To depict the true picture of genetic variation among the parental genotypes, molecular diversity analysis was also carried out using genomic-simple sequence repeats before crossing to ascertain that sufficient variability is present among the parents. The molecular analysis grouped the parental genotypes into four clusters (I–IV). The analysis of variance revealed that all the treatments were significant for most of the traits. The combining ability analysis suggested Pusa A-4 as the best general combiner for earliness, Pusa Bhindi-5 for high yield, and DOV-92 for fruit length, plant height, yield per plant, and coefficient of infection for Yellow Vein Mosaic Virus Disease resistance. Similarly, the specific combining ability analysis suggested that the cross combinations DOV-92 × Pusa Bhindi-5 followed by DOV-92 × Pusa A-4 and DOV-92 × Pusa Sawani exhibit high economic heterosis for yield per plant as well as for disease resistance. Finally, estimation of the degree of dominance and predictability ratio was also worked out which indicated the prevalence of non-additive gene action for most of the traits pointing towards sufficient scope for heterosis breeding in Okra.

Keywords

combining abilitygenetic diversityheritabilityheterosisOkrasimple sequence repeatsvirus resistance
Type
Research Article
Information
Plant Genetic Resources , Volume 18 , Issue 5 , October 2020 , pp. 316 - 329
Copyright
Copyright © The Author(s), 2020. Published by Cambridge University Press on behalf of NIAB

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