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Current approaches for the authentication of medicinal Dendrobium species and its products

Published online by Cambridge University Press:  12 February 2007

Yan-Bo Zhang ,
Paul Pui-Hay But ,
Zheng-Tao Wang  and
Pang-Chui Shaw
Yan-Bo Zhang
Affiliation:
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
Paul Pui-Hay But
Affiliation:
Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China Department of Biology, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
Zheng-Tao Wang
Affiliation:
Department of Pharmacognosy, Chinese Pharmaceutical University, Nanjing, China
Pang-Chui Shaw*
Affiliation:
Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China
*
*Corresponding author: E-mail: [email protected]
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Abstract

Dendrobium Sw., a member of the family Orchidaceae, includes approximately 1100 species distributed in different parts of the world. In China, the genus is represented by 76 species and two varieties, of which D. loddigesii, D. fimbriatum, D. chrysanthum, D. officinale (=D. candidum) and D. nobile are listed in the Chinese Pharmacopoeia as the source material for Herba Dendrobii (Shihu). Because of increased demand and high price, Herba Dendrobii is often adulterated in the trade by other related species. Many Dendrobium species are over-collected from the wild and are listed under endangered taxa in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). Therefore, a reliable authentication method is needed to regulate the trade and help conserve the species from unscrupulous collections. This review describes the present status of using medicinal Dendrobium in China, current approaches to authenticate medicinal Dendrobium plants and Herba Dendrobii, and the efforts under way towards the development of DNA microarrays to facilitate differentiation/identification of genuine material in complex Chinese medicinal formulations.

Keywords

Chinese medicinal material formulationDendrobiumDNA microarrayDNA sequencingFengdouHerba DendrobiiITS rRNA geneOrchidaceae
Type
Research Article
Information
Plant Genetic Resources , Volume 3 , Issue 2 , August 2005 , pp. 144 - 148
Copyright
Copyright © NIAB 2005

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