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Cryopreservation of in vitro-grown shoot tips of strawberry by the vitrification method using aluminium cryo-plates

Published online by Cambridge University Press:  28 November 2011

Shin-ichi Yamamoto ,
Kuniaki Fukui ,
Tariq Rafique ,
Nayyar Iqbal Khan ,
Carlos Roman Castillo Martinez ,
Kentaro Sekizawa ,
Toshikazu Matsumoto  and
Takao Niino
Shin-ichi Yamamoto
Affiliation:
Genebank, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan
Kuniaki Fukui
Affiliation:
Genebank, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan
Tariq Rafique
Affiliation:
Plant Genetic Resources Programme, National Agricultural Research Center, Islamabad, Pakistan
Nayyar Iqbal Khan
Affiliation:
NWFP Agricultural Research System, Abottabad, Pakistan
Carlos Roman Castillo Martinez
Affiliation:
National Genetic Resources Center, INIFAP, Tepatitlan, Mexico
Kentaro Sekizawa
Affiliation:
National Center for Seeds and Seedlings, Tsukuba 305-0852, Japan
Toshikazu Matsumoto
Affiliation:
Faculty of Life and Environmental Science, Shimane University, Matsue 690-1102, Japan
Takao Niino*
Affiliation:
Genebank, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan
*
*Corresponding author. E-mail: [email protected]
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Abstract

Cryopreservation using an aluminium cryo-plate was successfully applied to in vitro-grown strawberry (Fragaria × ananassa Duch.) shoot tips. The shoots were cold-hardened at 5°C for 3 weeks with an 8-h photoperiod. The shoot tips (1.5–2.0 mm × 0.5–1.0 mm) were dissected from the shoot and pre-cultured at 5°C for 2 d on Murashige and Skoog medium containing 2 M glycerol and 0.3 M sucrose. The pre-cultured shoot tips were placed on the aluminium cryo-plate containing ten wells embedded in alginate gel. Osmoprotection was performed by immersing the cryo-plates in a loading solution (2 M glycerol and 0.8 M sucrose) for 30 min at 25°C. Dehydration was performed by immersing the cryo-plates in plant vitrification solution 2 for 50 min at 25°C. Then, the cryo-plate with shoot tips was transferred into an uncapped cryotube that was held on a cryo-cane and directly immersed into liquid nitrogen (LN). After storage in LN, shoot tips attached to the cryo-plate were directly immersed into 2 ml of a 1 M sucrose solution for regeneration. Using this procedure, the average regrowth level of vitrified shoot tips of 15 strawberry cultivars reached 81%. This new method has many advantages and will facilitate the cryostorage of strawberry germplasm.

Keywords

aluminium platecryo-platestrawberryV-Cryo-plate methodvitrification
Type
Research Article
Information
Plant Genetic Resources , Volume 10 , Issue 1 , April 2012 , pp. 14 - 19
Copyright
Copyright © NIAB 2011

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References

Hirai, D and Sakai, A (1999) Cryopreservation of in vitro-grown meristems of potato (Solanum tuberosum L.) by encapsulation–vitrification. Potato Research 42: 153–160.CrossRefGoogle Scholar
Hirai, D and Sakai, A (2003) Simplified cryopreservation of sweet potato (Ipomoea batatas (L.) Lam.) by optimizing conditions for osmoprotection. Plant Cell Reports 21: 961–966.CrossRefGoogle ScholarPubMed
Hirai, D, Shirai, K, Shirai, S and Sakai, A (1998) Cryopreservation of in vitro-grown meristems of strawberry (Fragaria × ananassa Duch.) by encapsulation–vitrification. Euphytica 101: 109–115.CrossRefGoogle Scholar
Karen, LC and Kartha, KK (2009) Recovery of plants from pea and strawberry meristems cryopreserved for 28 years. CryoLetters 30: 41–46.Google Scholar
Kartha, KK, Leung, NL and Pahl, K (1980) Cryopreservation of strawberry meristems and mass propagation of plantlets. Journal of American Society of Horticultural Science 105: 481–484.CrossRefGoogle Scholar
Kim, HH, Yoon, JW, Pak, SU, Lee, SC, Baek, HJ, Cho, EG and Engelmann, F (2009) Development of alternative loading solutions in droplet-vitrification procedures. CryoLetters 30: 291–299.Google ScholarPubMed
Murashige, T and Skoog, F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiolgia Plantarum 15: 473–479.CrossRefGoogle Scholar
Navatel, J and Capron, M (1997) Cryopreservation of alginate-coated strawberry axillary buds. Acta Horticulturae 439: 659–662.CrossRefGoogle Scholar
Niino, T (2006) Developments in plant genetic resources cryopreservation technologies. In: Jung-Hoon Kang, (ed.) Effective Genebank Management in APEC Member Economies. Suwon: NIAB, pp. 197–217.Google Scholar
Niino, T, Tanaka, D, Ichikawa, S, Takano, J, Ivette, S, Shirata, K and Uemura, M (2003) Cryopreservation of in vitro-grown apical shoot tips of strawberry by vitrification. Plant Biotechnology 20: 75–80.CrossRefGoogle Scholar
Niino, T, Tanaka, D, Tantely, RR, Fukui, K and Shirata, K (2007) Cryopreservation of basal stem buds of in vitro-grown mat rush (Juncus spp.) by vitrification. CryoLetters 28: 197–206.Google ScholarPubMed
Nishizawa, S, Sakai, A, Amano, Y and Matsuzawa, T (1992) Cryopreservation of asparagus (Asparagus officinalis L.) embriogenic suspension cells and subsequent plant regeneration by a simple freezing method. CryoLetters 13: 379–388.Google Scholar
Pinker, I, Halmagyi, A and Olbricht, K (2009) Effects of sucrose preculture on cryopreservation by droplet-vitrification of strawberry cultivars and morphological stability of cryopreserved plants. CryoLetters 30: 202–211.Google ScholarPubMed
Reed, BM and Hummer, K (1995) Conservation of germplasm of strawberry (Fragaria species). In: Bajaj, YPS (ed.) Biotechnology in Agriculture and Forestry: Cryopreservation of Plant Germplasm I. Berlin: Springer-Verlag, pp. 323–343.Google Scholar
Sakai, A, Kobayashi, S and Oiyama, I (1990) Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. Var. brasiliensis Tanaka) by vitrification. Plant Cell Reports 9: 30–33.CrossRefGoogle ScholarPubMed
Sakai, A, Hirai, D and Niino, T (2008) Development of PVS-based vitrification and encapsulation–vitrification protocols. In: Reed, B (ed.) Plant Cryopreservation: A Practical Guide. New York: Springer LLC, pp. 33–58.CrossRefGoogle Scholar
Sekizawa, K, Yamamoto, S, Rafique, T, Fukui, K and Niino, T (2011) Cryopreservation of in vitro-grown shoot tips of carnation (Dianthus caryophyllus L.) by vitrification method using aluminium cryo-plates. Plant Biotechnology 28: 401–405.CrossRefGoogle Scholar
Yamamoto, S, Rafique, T, Priyantha, WS, Fukui, K, Matsumoto, T and Niino, T (2011a) Development of a cryopreservation procedure using aluminium cryo-plates. CryoLetters 32: 256–265.Google ScholarPubMed
Yamamoto, S, Rafique, T, Fukui, K, Sekizawa, K and Niino, T (2011b) V-Cryo-plate procedure as an effective protocol for cryobanks. Case study of mint cryopreservation. CryoLetters (in press).Google Scholar