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Characterization, genetic improvement and cultivation of Chlorophytum borivilianum—an important medicinal plant of India

Published online by Cambridge University Press:  12 February 2007

Satyabrata Maiti  and
K.A. Geetha
Satyabrata Maiti*
Affiliation:
National Research Centre for Medicinal and Aromatic Plants, Boriavi 387 310, Anand, Gujarat, India
K.A. Geetha
Affiliation:
National Research Centre for Medicinal and Aromatic Plants, Boriavi 387 310, Anand, Gujarat, India
*
*Corresponding author: E-mail: [email protected]
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Abstract

Chlorophytum borivilianum is an important medicinal plant known as ‘Safed musli’, used in many Ayurvedic vital tonics and aphrodisiac formulations. The species was first described from India in 1954 and reached rare status in nature due to overexploitation. Owing to its increased demand, the species has attracted the attention of farmers as well as researchers in several institutions. The present paper deals with various research aspects such as conservation biology, cytology, chemistry, plant genetic resources, micropropagation, crop management, etc. conducted on the crop for the last two decades in India. The species is diploid 2n=4x=28 and is mainly vegetatively propagated. Seeds remain dormant for nearly 10 months and also suffer from poor germination. Fleshy roots of the species contain saponins having therapeutic value. Germplasm collections made from different states in India show the occurrence of wide genetic variability in terms of plant type, maturity period, growth and yield characters, and size and shape of fleshy roots. Although the species is cross-pollinated in nature, self-pollination is also feasible artificially. One high-yielding cultivar and two high-yielding morphotypes have been recently identified. A micropropagation protocol using different explants has been standardized and agrotechnology for cultivation developed. Successful use of synthetic seed has also been reported. The article presents a comprehensive overview of the current state of research in the species with due emphasis on future thrust and possibilities.

Keywords

Chlorophytum borivilianumchromosomal statuscultivationgenetic diversitymicropropagationseed dormancyspononinsynthetic seed
Type
Research Article
Information
Plant Genetic Resources , Volume 3 , Issue 2 , August 2005 , pp. 264 - 272
Copyright
Copyright © NIAB 2005

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References

Aparna, V (2000) Evaluation of reproductive patterns and clonal propagation of two rare taxa for biodiversity conservation. PhD Thesis, Sardar Patel University, Gujarat, India.Google Scholar
Arora, DK, Suri, SS, Ramawat, KG and Merillon, JM (1999) Factors affecting somatic embryogenesis in long term callus cultures of Safed musli (Chlorophytum borivilianum), an endangered wonder herb. Indian Journal of Experimental Biology 37: 75–82.Google Scholar
Aundhe, DJ and Deokule, SS (2001) Studies on root morphology of Chlorophytum Ker-Gawl. Journal of Phytological Research 14: 195–197.Google Scholar
Bentham, G and Hooker, JD (1880) Genera Plantarum, Vol. 3, 2nd Delhi: Periodical Experts Book Agency Indian reprint 1988.Google Scholar
Bhagat, C and Jadeja, GC (2003) Variation and correlation in root yield and biochemical traits of Safedmusli (Chlorophytum borivilianum). Journal of Medicinal and Aromatic Plant Sciences 25: 33–36.Google Scholar
Bordia, PC and Jat, RD (1990) Comparative performance of transplanted seedlings of Safed musli (Chlorophytum species) from sexual and asexual means. Crop Breeding Newsletter 1: 14–15.Google Scholar
Bordia, PC, Joshi, A and Simlot, MM (1995) Safed musli. In: Chadha, KL, Gupta, R (eds) Advances in Horticulture, Vol. 11 Medicinal and Aromatic Plants. New Delhi: Malhotra Publishing House pp. 429–451.Google Scholar
Chandra, R, Kumar, D, Aishwath, OP and Jha, BK (2003 a) Response of Chlorophytum borivilianum to organic manure. Journal of Tropical Medicinal Plants 4: 261–264.Google Scholar
Chandra, R, Kumar, D and Jha, BK (2003 b) Growth and yield of safed musli (Chlorophytum borivilianum) as influenced by spacing. Indian Journal of Agricultural Sciences 73: 153–155.Google Scholar
Chopra, RN, Nayar, SL and Chopra, IC (1956) Glossary of Indian Medicinal Plants, reprinted 1992. New Delhi: CSIR.Google Scholar
Cooke, T (1908) Flora of the Presidency of Bombay, Vol. 2, Indian edn 1997. Dehra Dun, India: Bishen Singh Mahendra Pal Singh, pp. 771–774.Google Scholar
Darlington, CD and Wylie, AP (1955) Chromosome Atlas of Flowering Plants, 2nd edn. London: George Allan and Unwin.Google Scholar
Dave, A, Bilochi, G and Purohit, SD (2003 a) Scaling-up production and field performance of micropropagated medicinal herb ‘Safed Musli’ (Chlorophytum borivilianum). In Vitro Cellular & Developmental Biology—Plant 39: 419–424.Google Scholar
Dave, A, Joshi, N and Purohit, SD (2003 b) In vitro propagation of Chlorophytum borivilianum using encapsulated shoot buds. European Journal of Horticultural Science 69: 37–42.Google Scholar
Gaikwad, PD, John, SC and Billore, M (2003) Callus induction and regeneration studies in safed musli (Chlorophytum borivilianum Sant & Fern). Research on Crops 4: 249–253.Google Scholar
Geetha, KA and Maiti, S (2001) Reproductive biology of Chlorophytum borivilianum. Journal of Tropical Medicinal Plants 2: 287–290.Google Scholar
Geetha, KA and Maiti, S (2002) Biodiversity in Chlorophytum borivilianum Santapau and Fernandes. Plant Genetic Resources Newsletter 129: 52–53.Google Scholar
Geetha, KA and Maiti, S (2004) New basic chromosome number in Chlorophytum borivilianum Santapau and Fernandes. Journal of Spices and Aromatic Crops 13: 121–123.Google Scholar
Hooker, JD (1894) The Flora of British India, New Edition, Vol. 6, reprinted 1997. Dehra Dun, India: Bishen Singh Mehendra Pal Singh.Google Scholar
Jat, RD (1993) Study on variability and micropropagation in Safed musli (Chlorophytum borivilianum). MSc Thesis, Rajasthan College of Agriculture, Udaipur, India.Google Scholar
Jat, RD and Sharma, GS (1996) Genetic variability and correlation in Safed musli (Chlorophytum borivilianum). Journal of Medicinal and Aromatic Plant Sciences 18: 496–498.Google Scholar
John, CK, Somani, VJ and Thengane, RJ (1989) Some interesting features of male meiosis in Chlorophytum tuberosum Baker. Current Science 58: 254–255.Google Scholar
Joshi, A, Ganesh, R, Sharma, A and Sharma, GS (1999) Plant regeneration from stem-disc derived callus cultures of Chlorophytum borivilianum. Annals of Plant Physiology 13: 79–83.Google Scholar
Joshi, A, Ganesh, R, Sharma, A and Audichya, P (2000) Sapogenine content determination in Chlorophytum borivilianum. Bionature 20: 47–49.Google Scholar
Kothari, SK (2004) Safed musli (Chlorophytum borivilianum) revisited. Journal of Medicinal and Aromatic Plant Sciences 26: 60–63.Google Scholar
Kothari, SK and Singh, K (2001) Evaluation of Safed musli (Chlorophytum borivilianum Santapau and Fernandes) germplasm. Journal of Spices and Aromatic Crops 10: 147–149.Google Scholar
Kothari, SK and Singh, K (2003) Production techniques for the cultivation of Safed musli (Chlorophytum borivilianum). Journal of Horticultural Science and Biotechnology 78: 261–264.Google Scholar
Kumar, LSS and Rao, HKS (1958) Chromosome number in Chlorophytum tuberosum Baker. Current Science 27: 406–407.Google Scholar
Lavania, UC, Basu, S, Srivastava, S, Mukai, Y and Lavania, S (2005) In situ chromosomal localization of rDNA sites in ‘Safed musli’ Chlorophytum Ker-Gawl and their physical measurement by Fiber FISH. Journal of Heredity 96: 155–160.Google Scholar
Mandal, K, Maiti, S, Saxena, DR and Saxena, M (2004) A new leaf spot disease of Safed musli. Journal of Mycology and Plant Pathology 34: 162–163.Google Scholar
Murashige, T and Skoog, F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473–497.Google Scholar
Murthy, SKR (2004) Bhavaprakasanighantu of Sri Bhavamisra, Vol. 1, English translation. Varanasy, India: Chowkhamba Krishnadas Academy.Google Scholar
Nadkarni, AK (1954) Indian Materia Medica. Delhi: Asiatic Publishing House.Google Scholar
Naik, VN (1977 a) Erroneous report of the new basic chromosome number in Chlorophytum. Science and Culture 43: 260–261.Google Scholar
Naik, VN (1977 b) Cytotaxonomic studies of six Indian species of Chlorophytum. Botanical Journal of the Linnaeus Society 74: 297–308.Google Scholar
Naik, VN and Nirgude, SM (1981) Anatomy in relation to taxonomy of Chlorophytum (Liliaceae). Indian Journal of Botany 4: 48–60.Google Scholar
Nayar, MP and Sastry, ARK (1988) Red Data Book of Indian Medicinal Plants, Calcutta: Botanical Survey of India.Google Scholar
Oudhia, P (2000) Record of orange banded blister beetle, Zonabris pustulata Thunb. [ Mylabris pustulata ] (Coleoptera: Meloidae) on safed musli (Chlorophytum borivilianum). Insect Environment 6: 138Google Scholar
Oudhia, P (2001) Problems perceived by safed moosli (Chlorophytum borivilianum) growers of Chhattisgarh (India) region: a study. Journal of Medicinal and Aromatic Plant Sciences 22/23 396–399.Google Scholar
Pahuja, AN and Kumar, V (1969) Cytogeography of three species of Chlorophytum. Current Science 38: 469–471.Google Scholar
Pandey, R (2003) Root-knot disease of safed musli. Indian Journal of Nematology 33: 64Google Scholar
Paturde, JT, Wankhade, SG, Khode, PP, Chatol, PU, Deo, DD and Bhuyar, SA (2002) Effect of organic manures and plant population on yield of safed musli (Chlorophytum borivilianum). Agricultural Science Digest 22: 51–52.Google Scholar
Pudake, RN and Dhumale, DB (2003) In vitro multiplication of Chlorophytum borivilianum Sant and Fern.. Journal of Maharashtra Agricultural University 28: 265–267.Google Scholar
Purohit, SD, Dave, A and Kukda, G (1994 a) Micropropagation of Safed musli (Chlorophytum borivilianum), a rare Indian medicinal herb. Plant Cell, Tissue and Organ Culture 39: 93–96.Google Scholar
Purohit, SD, Dave, A and Tyagi, YD (1994 b) Chlorophytum borivilianum Sant. & Fern. (Liliaceae): an interesting species from Aravalies in Rajasthan. Rheedia 4: 113–115.Google Scholar
Qiu, SX Li, XC, Xiong YS, Dong, YM, Chai, HB, Fransworth, NR, Pezzuto, JM and Fong, HHS (2000) Isolation and characterization of cytotoxic saponin chloromaloside-A from Chlorophytum malayense. Planta Medica 66: 587–590.Google Scholar
Raghavan, SR, Ansari, MY and Kamble, SY (1977) Cytotaxaonomic studies in Chlorophytum Ker-Gawl. (Liliaceae). Current Science 46: 534–535.Google Scholar
Santapau, H and Fernandes, RR (1954) A new species of Chlorophytum from Salsette Island. Journal of the Bombay Natural Historical Society 52: 896–900.Google Scholar
Shah, GL (1978) Flora of Gujarat State Part II. Vallabh Vidyanagar, India: Sardar Patel University.Google Scholar
Sheriff, A and Chennaveeraiah, MS (1972) Karyomorphology of four diploid species of Chlorophytum. Nucleus 15: 45Google Scholar
Shrivastava, DK, Mishra, PK, Gangrade, SK and Verma, S (2001) Studies on propagation methods and dormancy in safed musli (Chlorophytum spp.). Journal of Medicinal and Aromatic Plant Sciences 22/23, 275–276.Google Scholar
Singh, A and Chauhan, HS (2003) Safed musli (Chlorophytum borivilianum): distribution, biodiversity and cultivation. Journal of Medicinal and Aromatic Plant Sciences 25: 712–719.Google Scholar
Singh, A, Khanuja, SPS, Singh, S, Singh, A and Singh, A (2003) Agronomic practices for the production of Safed musli (Chlorophytum borivilianum Santapau and Fernandes). Natural Products Radiance 2: 308–313.Google Scholar
Singh, A, Singh, S, Patra, DD, Singh, M, Arya, SJK and Khanuja, SPS (2004) Cultivation and processing technologies of safed musli (Chlorophytum borivilianum). Journal of Medicinal and Aromatic Plant Sciences 26: 70–76.Google Scholar
Singh, HB, Singh, A, Tripathi, A, Tiwari, SK and Johri, JK (2001) Collar rot of Chlorophytum borivilianum caused by Corticium rolfsii: a new disease. Bulletin OEPP 31: 112–113.Google Scholar
Sivarajan, VV and Balachandran, I (1994) Ayurvedic Drugs and Their Sources. New Delhi: Oxford and IBH Publishing Co.Google Scholar
Suri, SS, Jain, S, Arora, DK and Ramawat, KG (1999) In vitro high frequency of plantlets and tuberous root formation in Chlorophytum borivilianum. Gartenbauwissenschaft 64: 106–110.Google Scholar
Tandon, M and Shukla, YN (1992) Sapogenins from Asparagus adscendens and Chlorophytum arundinaceum. Journal of the Indian Chemical Society 69: 893Google Scholar
Tandon, M and Shukla, YN (1995) Phytoconstituents of Asparagus adscendens, Chlorophytum arundinaceum and Curculigo orchioides: a review. Current Research on Medicinal and Aromatic Plants 17: 202–210.Google Scholar
Tandon, M and Shukla, YN (1996) Arundinoside B, a new acylated glucoside from Chlorophytum arundinaceum. Journal of the Indian Chemical Society 35: 988–989.Google Scholar
Tandon, M and Shukla, YN (1997) Arundinoside A—a new spirostane from Chlorophytum arundinaceum. Journal of the Indian Chemical Society 74: 56–58.Google Scholar
Tandon, M, Shukla, YN and Thakur, RS (1992) 4-Hydroxy-8,11-oxidoheneicosanol and other constituents from Chlorophytum arundinaceum roots. Phytochemistry 31: 2525–2526.Google Scholar
Von Steiner, M and Holtze, H (1954) Triterpene and triterpine saponin. In: Peach, K, Tracey, MV (eds) Modern Method of Plant Analysis, Vol. 3. Berlin: Springer Verlag, p.64.Google Scholar
Xing-Cong, Li, De-Zu, Wang, Chong-Re, Yang (1990) Steroidal saponins from Chlorophytum malayense. Phytochemistry 29: 3893–3898.Google Scholar