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Broccoli ‘Fiolaro’ (Brassica oleracea)an endangered typical Italian cultivar. A genetic analysis by SSR markers

Published online by Cambridge University Press:  16 May 2008

M. Vischi ,
M. Fiori ,
E. De Paoli ,
S. Padovan ,
M. Guarda  and
A. Olivieri
M. Vischi*
Affiliation:
Department of Agriculture and Environmental Sciences, University of Udine, Via delle Scienze 208, 33100 Udine, Italy
M. Fiori
Affiliation:
Department of Agriculture and Environmental Sciences, University of Udine, Via delle Scienze 208, 33100 Udine, Italy
E. De Paoli
Affiliation:
Department of Agriculture and Environmental Sciences, University of Udine, Via delle Scienze 208, 33100 Udine, Italy
S. Padovan
Affiliation:
Institute of Genetics and Agricultural Experimentation ‘N. Strampelli’, Via Marconi 1, 36045 Lonigo, Vicenza, Italy
M. Guarda
Affiliation:
Institute of Genetics and Agricultural Experimentation ‘N. Strampelli’, Via Marconi 1, 36045 Lonigo, Vicenza, Italy
A. Olivieri
Affiliation:
Department of Agriculture and Environmental Sciences, University of Udine, Via delle Scienze 208, 33100 Udine, Italy
*
*Corresponding author. E-mail: [email protected]
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Abstract

‘Broccolo fiolaro’ (Brassica oleracea L. convar.Italica) is a typical vegetable produced in a restricted hill country area around Creazzo(Vicenza) in north-eastern Italy. The cultivation of this vegetable dates back severalcenturies ago, but a very few farms are still involved in the production.‘Broccolo fiolaro’ is a variety of broccoli highly valued for itsagronomic and organoleptic features.

Four ‘Broccolo fiolaro’ selections were characterized by simplesequence repeats (SSRs) molecular markers and compared with other five cultivatedvarieties of broccoli of the Italica group in order to reconstruct their genetic structureand evaluate the degree of similarity among and within populations. The analysis of 12 SSRpolymorphic loci pointed out a low genetic variability among the four ‘Broccolofiolaro’ selections. Moreover, the whole ‘Fiolaro’ groupshowed significant differentiation from the other broccoli cultivars and could be easilydistinguished by cluster analysis. An assignment test on ~30 genotyped plants randomlychosen from each broccoli population correctly recognized the proper accession 88% of thetime, proving this method to be useful for cultivar identification.

Keywords

alleleBroccolo fiolarosimple sequence repeats
Type
Research Article
Information
Plant Genetic Resources , Volume 6 , Issue 3 , December 2008 , pp. 215 - 221
Copyright
Copyright © NIAB 2008

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