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Aphid feeding response and microsatellite-based genetic diversity amongdiploid Brachypodium distachyon (L.) Beauv accessions

Published online by Cambridge University Press:  01 April 2009

Perumal Azhaguvel ,
Wanlong Li ,
Jackie C. Rudd ,
Bikram S. Gill ,
G. J. Michels Jr.  and
Yiqun Weng
Perumal Azhaguvel
Affiliation:
Texas AgriLife Research, 6500 Amarillo Boulevard West, Amarillo, TX 79106, USA
Wanlong Li
Affiliation:
Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA
Jackie C. Rudd
Affiliation:
Texas AgriLife Research, 6500 Amarillo Boulevard West, Amarillo, TX 79106, USA
Bikram S. Gill
Affiliation:
Department of Plant Pathology, Kansas State University, Manhattan, KS 66506, USA
G. J. Michels Jr.
Affiliation:
Texas AgriLife Research, 6500 Amarillo Boulevard West, Amarillo, TX 79106, USA
Yiqun Weng*
Affiliation:
Texas AgriLife Research, 6500 Amarillo Boulevard West, Amarillo, TX 79106, USA
*
*Corresponding author. E-mail: [email protected]
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Abstract

False brome grass, Brachypodium distachyon (L.) Beauv, has been proposedas a new model species to bridge rice and temperate cereal crops for genomics research.However, much basic information for this species is still lacking. In this study, sixdiploid B. distachyon(2n = 2x = 10)accessions (Bd1-1, Bd2-3, Bd3-1, Bd18-1, Bd21 and BD29) were evaluated for their responseto infestation by two cereal aphid pests of common wheat (Triticumaestivum L.): the greenbug, Schizaphis graminum Rondani, and theRussian wheat aphid (RWA), Diuraphis noxia Mordvilko. Through databasemining of B. distachyon expressed sequence tag (EST) and genomic DNAsequences, 160 EST- and 21 genomic microsatellite markers were developed and used toevaluate genetic diversity among the B. distachyon accessions. All sixaccessions were resistant to RWA biotype RWA1 but showed distinct responses to feeding bygreenbug biotypes C and E, as well as RWA2 RWAs. Although microsatellite-based geneticdiversity among different accessions was generally low, Bd1-1 and BD29 were the mostdiverged from the other four lines. The genetic divergence was correlated withgeographical distances between the Brachypodium accessions. Comparison ofsimple sequence repeat polymorphisms in three inbred lines (Bd2-3, Bd3-1 and Bd18-1) withtheir respective original parental lines revealed no effect of inbreeding on geneticdiversity. Phylogenetic analysis suggested that Aegilops tauschii (Coss.)Schmal., the D genome donor of common wheat, was closer to B. distachyonthan to rice. The greenbug - B. distachyon system seemsto be a model of choice for plant–aphid interaction studies in the grassgenome.

Keywords

aphid resistanceBrachypodium distachyonDiuraphis noxiagenetic diversitymicrosatelliteSchizaphis graminum
Type
Research Article
Information
Plant Genetic Resources , Volume 7 , Issue 1 , April 2009 , pp. 72 - 79
Copyright
Copyright © NIAB 2008

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