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Allele mining in the gene pool of wild Solanum species for homologues of late blight resistance gene RB/Rpi-blb1

Published online by Cambridge University Press:  21 February 2011

Artem Pankin ,
Ekaterina Sokolova ,
Elena Rogozina ,
Maria Kuznetsova ,
Kenneth Deahl ,
Richard Jones  and
Emil Khavkin
Artem Pankin*
Affiliation:
Institute of Agricultural Biotechnology, Moscow, Russia
Ekaterina Sokolova
Affiliation:
Institute of Agricultural Biotechnology, Moscow, Russia
Elena Rogozina
Affiliation:
Institute of Plant Industry, St. Petersburg, Russia
Maria Kuznetsova
Affiliation:
Institute of Phytopathology, Bol'shiye Vyazemy, Russia
Kenneth Deahl
Affiliation:
USDA-ARS, Agriculture Research Center, Beltsville, MD, USA
Richard Jones
Affiliation:
USDA-ARS, Agriculture Research Center, Beltsville, MD, USA
Emil Khavkin
Affiliation:
Institute of Agricultural Biotechnology, Moscow, Russia
*
*Corresponding author. E-mail: [email protected]
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Abstract

A coiled coil-nucleotide binding site-leucine-rich repeat gene RB/Rpi-blb1 isolated from Solanum bulbocastanum confers broad-spectrum resistance to Phytophthora infestans and is currently employed in potato breeding for durable late blight (LB) resistance. RB homologues were reported in several Solanum species; some of them retained defence function. Here, we report additional evidence on RB-like sequences in 21 Solanum species of the section Petota. The panel of Solanum species was screened with three RB-related PCR markers. RB-like sequences were found in every tested Solanum accession, suggesting universal distribution of RB structural homologues among Solanum genomes, while locus-specific RB-629 was found only in 15 species. Phylogenetic analysis of RB-629 sequences suggested a highly conservative pattern of polymorphisms that was neither species- nor series-specific. Apparently, duplication and evolution of RB-like loci preceded Solanum speciation. Marker presence and particular haplotypes were not immediately associated with high LB resistance.

Keywords

late blight resistancePhytophthora infestanspotatoR genesSolanum spp.
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 2 , July 2011 , pp. 305 - 308
Copyright
Copyright © NIAB 2011

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