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AFLP marker diversity in water yam (Dioscorea alata L.)

Published online by Cambridge University Press:  08 March 2007

C. N. Egesi ,
R. Asiedu ,
G. Ude ,
S. Ogunyemi  and
J. K. Egunjobi
C. N. Egesi*
Affiliation:
National Root Crops Research Institute, xyaUmudike, PMB 7006, Umuahia 440001, Abia State, Nigeria
R. Asiedu
Affiliation:
International Institute of Tropical Agriculture, c/o L. W. Lambourn & Co., Carolyn House, 26 Dingwall Road, Croydon, CR9 3EE, UK
G. Ude
Affiliation:
Department of Natural Science, Bowie State University, Bowie, MD 20715, USA
S. Ogunyemi
Affiliation:
Department of Crop Protection and Environmental Biology, University of Ibadan, Ibadan, Nigeria
J. K. Egunjobi
Affiliation:
Department of Crop Protection and Environmental Biology, University of Ibadan, Ibadan, Nigeria
*
* E-mail: [email protected]
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Abstract

Amplified fragment length polymorphism (AFLP) markers were employed to assess intraspecific variability in water yam (Dioscorea alata L.). Fifty-three accessions of diverse geographic origins in West and Central Africa (Benin, Côte d'Ivoire, Ghana, Nigeria and Chad) and Puerto Rico were analysed using three AFLP primer combinations. Of the 342 amplification products generated, a mean of 53.4% were polymorphic. Genetic similarity coefficients ranged from 0.29 to 0.95. The accessions could be clustered into three major genetic groups with one outlier. Each group was a mixture of accessions of different geographical origin indicating that geography has not played a major role in the differentiation of the species. A few accessions clustered very tightly suggesting that there may be duplicate accessions in the collection. The wide genetic variation observed constitutes a good basis for genetic improvement of yam.

Keywords

amplified fragment length polymorphismDioscorea alataDNA fingerprintinggenetic variabilitywater yam
Type
Research Article
Information
Plant Genetic Resources , Volume 4 , Issue 3 , December 2006 , pp. 181 - 187
Copyright
Copyright © NIAB 2006

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