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Starch metabolism mutants in barley: A TILLING approach

Published online by Cambridge University Press:  16 March 2011

Riccardo Bovina ,
Valentina Talamè ,
Salvi Silvio ,
Maria Corinna Sanguineti ,
Paolo Trost ,
Francesca Sparla  and
Roberto Tuberosa
Riccardo Bovina
Affiliation:
Department of Agroenvironmental Science and Technology, University of Bologna, Bologna, Italy
Valentina Talamè
Affiliation:
Department of Agroenvironmental Science and Technology, University of Bologna, Bologna, Italy
Salvi Silvio
Affiliation:
Department of Agroenvironmental Science and Technology, University of Bologna, Bologna, Italy
Maria Corinna Sanguineti
Affiliation:
Department of Agroenvironmental Science and Technology, University of Bologna, Bologna, Italy
Paolo Trost
Affiliation:
Department of Experimental Evolutionary Biology, University of Bologna, Bologna, Italy
Francesca Sparla
Affiliation:
Department of Experimental Evolutionary Biology, University of Bologna, Bologna, Italy
Roberto Tuberosa*
Affiliation:
Department of Agroenvironmental Science and Technology, University of Bologna, Bologna, Italy
*
*Corresponding author. E-mail: [email protected]
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Abstract

In this study, the targetting-induced local lesions in genomes approach was used to identify mutants for genes related to starch metabolism in barley. Starch is the major reserve of plants and serves as primary carbohydrate component in human and livestock diets and has also numerous industrial applications. Mutants for biosynthetic or regulatory genes of starch metabolism often produce starch granules with abnormal morphological and molecular features that could be of interest for technological applications. We report the identification of 29 mutations in five starch-related barley genes (Bmy1, GBSSI, LDA1, SSI and SSII) through the molecular screening of TILLMore, a sodium azide-mutagenized population. Almost all the mutations detected were CG–TA transitions and several (c. 60%) implied a change in amino-acid sequence and therefore possible phenotypic effects. Four mutants showed non-sense or splice-junction alterations, which could drastically affect the protein function.

Keywords

Hordeum vulgarereverse-geneticsstarchTILLING
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 2 , July 2011 , pp. 170 - 173
Copyright
Copyright © NIAB 2011

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