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SSR marker-based DNA fingerprinting and cultivar identification of rice (Oryza sativa L.) in Punjab state of India

Published online by Cambridge University Press:  17 July 2009

Navraj K. Sarao ,
Yogesh Vikal ,
Kuldeep Singh ,
Monika A. Joshi  and
R. C. Sharma
Navraj K. Sarao*
Affiliation:
Seed Technology Centre, PAU, Ludhiana, Punjab141 004, India
Yogesh Vikal
Affiliation:
School of Agricultural Biotechnology, PAU, Ludhiana, Punjab141 004, India
Kuldeep Singh
Affiliation:
School of Agricultural Biotechnology, PAU, Ludhiana, Punjab141 004, India
Monika A. Joshi
Affiliation:
Seed Technology Centre, PAU, Ludhiana, Punjab141 004, India
R. C. Sharma
Affiliation:
Seed Technology Centre, PAU, Ludhiana, Punjab141 004, India
*
*Corresponding author. E-mail: [email protected]
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Abstract

In India, Protection of Plant Varieties and Farmer's Rights Act (PPV&FRA, 2001) requires the registration and protection of new and notified/extant plant varieties based on the criteria of distinctness, uniformity and stability (DUS) of morphological characteristics. However, these morphological traits have not been able to resolve closely related genotypes. The molecular markers can very well support the DUS testing in such cases. In the present study, therefore, 14 varieties of rice cultivated in Punjab state of India were fingerprinted using 75 simple sequence repeat (SSR) primers. Out of these, 58 primers produced polymorphic profiles, while 13 were monomorphic, 2 revealed null allele and the remaining 2 amplified only from super basmati. In a screen of 7 cultivars, 16 SSR loci produced 17 rare/unique alleles, which provided an opportunity for their unambiguous identification. Cluster analysis based on SSR data clearly distinguished the cultivars into two dist inct groups: comprising non-basmati (group I) and basmati (group II). The cluster pattern was consistent with the pedigree and breeding history of the cultivars.

Keywords

DNA fingerprintingOryza sativariceSSR markers
Type
Short Communication
Information
Plant Genetic Resources , Volume 8 , Issue 1 , April 2010 , pp. 42 - 44
Copyright
Copyright © NIAB 2009

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