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Reliability of germination testing of ex situ conserved seeds: a genebank case study on outsourced analyses

Published online by Cambridge University Press:  11 May 2012

Theo van Hintum  and
Rob van Treuren
Theo van Hintum*
Affiliation:
Centre for Genetic Resources, the Netherlands (CGN), Wageningen University and Research Centre, PO Box 16, NL-6700 AAWageningen, The Netherlands
Rob van Treuren
Affiliation:
Centre for Genetic Resources, the Netherlands (CGN), Wageningen University and Research Centre, PO Box 16, NL-6700 AAWageningen, The Netherlands
*
*Corresponding author. E-mail: [email protected]
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Abstract

Germination tests are performed on a routine basis to determine the viability of genebank accessions. The results determine which accessions have to be rejuvenated. The reliability of the germination test results used by the Centre for Genetic Resources, the Netherlands was determined by the retesting of 641 random samples anonymously, in the same year and by the same testing agency as the original tests. Results showed alarmingly low reliabilities, with error levels much higher than expected based on sampling effects. The result of a germination test of a random sample with a germination of 80% was shown to have a 95% confidence interval from 63 to 97%. The errors differed strongly over crops and testing years, and were larger for crop wild relatives than for crop species.

Keywords

error estimationgenebanksgenetic resourcesgerminationseed viability
Type
Short Communication
Information
Plant Genetic Resources , Volume 10 , Issue 2 , August 2012 , pp. 134 - 136
Copyright
Copyright © NIAB 2012

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References

Food and Agriculture Organization of the United Nations/International Plant Genetic Resources Institute (FAO/IPGRI), (1994) Genebank Standards. Rome: FAO/IPGRI, 17 p.Google Scholar
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