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Molecular mapping of linked leaf rust resistance and non-glaucousness gene introgressed from Aegilops tauschii Coss. in hexaploid wheat Triticum aestivum L.

Published online by Cambridge University Press:  16 January 2017

Manny Saluja ,
Satinder Kaur ,
Urmil Bansal ,
Subhash Chander Bhardwaj  and
Parveen Chhuneja
Manny Saluja
Affiliation:
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana-141004, Punjab, India
Satinder Kaur*
Affiliation:
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana-141004, Punjab, India
Urmil Bansal
Affiliation:
The University of Sydney Plant Breeding Institute-Cobbitty, PMB4011, Narellan, NSW2567, Australia
Subhash Chander Bhardwaj
Affiliation:
Regional Research Station, Indian Institute of Wheat and Barley Research, Flowerdale, Shimla, HP, India
Parveen Chhuneja
Affiliation:
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana-141004, Punjab, India
*
*Corresponding author. E-mail: [email protected]
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Abstract

Aegilops tauschii, the D genome donor of wheat, is an invaluable source of genetic variability, which can be utilized for broadening the wheat gene pool. Linked leaf rust resistance and non-glaucousness genes transferred from Ae. tauschii to cultivated wheat variety WH542 were mapped in the present study. Genetic analysis in an F2 population from a BC3 plant derived from the cross Triticum durum cv. PBW114/Ae. tauschii acc. pau14195//4*T. aestivum cv. WH542 revealed monogenic dominant inheritance for both the traits. The leaf rust resistance and the non-glaucousness gene were tentatively named LrT and IwT, respectively. Leaf rust resistance gene exhibited all stage resistance. SSR markers Xbarc124, Xgdm5, Xgdm35, Xcfd51 and EST-derived markers Xcau96 and Xte6 on chromosome 2DS were linked with both genes. Chromosomal assignments of the genes were confirmed by testing linked SSR markers on Chinese Spring nulli-tetrasomics lines. SSR markers Xcau96 (1.6 cM) and Xbarc124 (0.6 cM) flanked LrT and Xgdm35 (4.1 cM) and Xte6 (2.5 cM) flanked non-glaucousness gene. LrT and IwT showed a recombination distance of 3.4 cM. Hence, IwT can be used as an easy to score morphological marker of LrT during its transfer to other glaucous backgrounds.

Keywords

Aegilops tauschiibridging speciesgenetic mappingleaf rust resistancenon-glaucousnesswide hybridization
Type
Research Article
Information
Plant Genetic Resources , Volume 16 , Issue 1 , February 2018 , pp. 82 - 88
Copyright
Copyright © NIAB 2017 

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