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Molecular diversity in the Ukrainian melon collection as revealed by AFLPs and microsatellites

Published online by Cambridge University Press:  10 October 2008

Padmavathi Nimmakayala ,
Yan R. Tomason ,
Jooha Jeong ,
Gopinath Vajja ,
Amnon Levi ,
Paul Gibson  and
Umesh K. Reddy
Padmavathi Nimmakayala
Affiliation:
Department of Biology and Gus R. Douglas Institute, West Virginia State University, Institute, WV25112, USA
Yan R. Tomason
Affiliation:
Department of Biology and Gus R. Douglas Institute, West Virginia State University, Institute, WV25112, USA Department of Selection and Seed Production, Dnepropetrovsk State Agrarian University, Voroshilov 25, Dnepropetrovsk49600, Ukraine, e-mail: [email protected]
Jooha Jeong
Affiliation:
Department of Biology and Gus R. Douglas Institute, West Virginia State University, Institute, WV25112, USA
Gopinath Vajja
Affiliation:
Department of Biology and Gus R. Douglas Institute, West Virginia State University, Institute, WV25112, USA
Amnon Levi
Affiliation:
US Vegetable Laboratory, USDA, ARS, 2875 Savannah Highway, Charleston, SC29414, USA
Paul Gibson
Affiliation:
Department of Selection and Seed Production, Dnepropetrovsk State Agrarian University, Voroshilov 25, Dnepropetrovsk49600, Ukraine, e-mail: [email protected] Department of Plant, Soil and Agriculture Systems, Southern Illinois University, 62901Carbondale, IL, USA, e-mail: [email protected]
Umesh K. Reddy*
Affiliation:
Department of Biology and Gus R. Douglas Institute, West Virginia State University, Institute, WV25112, USA
*
*Corresponding author. E-mail: [email protected]
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Abstract

Thirty-eight melon accessions, which are of primary breeding importance in the Ukraine, were analysed for diversity. These collections represent a major non-US and non-western Europe source of melon germplasm that have not yet been subjected to molecular characterization. Molecular diversity was estimated based on a robust set of 465 polymorphisms gathered by amplified fragment length polymorphisms and simple sequence repeats (SSR). In this paper, we report 12 newly developed polymorphic SSR primer pairs, and their use for molecular characterization in the Ukrainian melon collections. Based on these polymorphisms, we estimated similarity indices that ranged from 0.70 to 1.00 among various accessions. The phylogenetic tree based on the similarity indices and a three-dimensional plot of the first three vectors of the principal component analysis corresponded fairly well with the existing three classical morphotypes namely aestivalis, europeus and hiemalis, under the convarEuropeus, which is also known as adana. The polymorphisms generated in the current study, which are specific to the grouping of fruit types and days to maturity will be very useful for further genetic studies and marker-assisted selections.

Keywords

AFLPCucumis melodiversitymelonSSR
Type
Research Article
Information
Plant Genetic Resources , Volume 7 , Issue 2 , August 2009 , pp. 127 - 134
Copyright
Copyright © NIAB 2008

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