Hostname: page-component-78c5997874-ndw9j Total loading time: 0 Render date: 2024-11-07T10:30:04.070Z Has data issue: false hasContentIssue false
  • English
  • Français

Modified AFLP technique for fingerprinting of Anemone coronaria

Published online by Cambridge University Press:  12 February 2007

J. Fang  and
T. Paz
J. Fang*
Affiliation:
Nanjing Agricultural University, Nanjing, 210095, China
T. Paz
Affiliation:
Agricultural Research Organization, The Volcani Center, P.O.B. 6, Bet-Dagan 50 250, Israel
*
*Corresponding author: Department of Botany and Plant Sciences, University of California–Riverside, Riverside, CA 92 521, USA. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

We successfully generated clear amplified fragment length polymorphism (AFLP) profiles of Anemone coronaria using only a single restriction enzyme (EcoR I). Four primer combinations generated 571 bands in 37 anemone accessions with an average 142.75 bands per primer combination. Polymorphism among the 37 samples was very high (98.15%). This high polymorphism showed that the one-enzyme AFLP method is effective for the assessment of genetic diversity as well as cultivar differentiation of anemone. We observed 26 bands which differentiated between fingerprints derived from leaf and corm DNA of eight plant individuals. However, the low percentage of such bands showed that anemone corms can also be used to evaluate anemone germplasm using AFLP markers.

Keywords

AFLPAnemone coronariafingerprintingmodificationpolymorphic bands
Type
Short communication
Information
Plant Genetic Resources , Volume 3 , Issue 1 , April 2005 , pp. 73 - 75
Copyright
Copyright © NIAB 2005

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

David, L, Rajasekaran, P, Fang, J, Hillel, J and Lavi, U (2001) Polymorphism in ornamental and common carp strains (Cyprinus carpio L.) as revealed by AFLP analysis and a set of microsatellite markers. Molecular Genetics and Genomics 266: 353–362.CrossRefGoogle Scholar
Horovitz, A (1973) Introduction of Wild Genes into Commercial Anemone coronaria L. Comparison Progress Report, U.S.–Israel Binational Science Foundation (BSF) Project 47. Israel, 37 pp.Google Scholar
Kashkush, K, Fang, J, Tomer, E, Hillel, J and Lavi, U (2001) Cultivar identification and genetic map of mango (Mangifera indica). Euphytica 122: 129–136.CrossRefGoogle Scholar
Lockhart, PJ and McLenacham, PA (1997) Isolating polymorphic plant DNA fragments identified using AFLP technology without acrylamide gels: markers for evolutionary studies. Focus 19: 70–71.Google Scholar
Murray, MG and Thompson, WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8: 4321–4325.CrossRefGoogle ScholarPubMed
Vos, P, Hogers, R, Bleeker, M, Reijans, M, Van de Lee, T, Hornes, M, Frijters, A, Pot, J, Peleman, J, Kuiper, M and Zabeau, M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23: 4407–4414.CrossRefGoogle ScholarPubMed