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The identification of a new gene for leaf pubescence introgressed into bread wheat from Triticum timopheevii Zhuk. and its manifestation in a different genotypic background

Published online by Cambridge University Press:  27 April 2021

A. V. Simonov Open the ORCID record for A. V. Simonov [Opens in a new window] ,
O. G. Smirnova Open the ORCID record for O. G. Smirnova [Opens in a new window] ,
M. A. Genaev Open the ORCID record for M. A. Genaev [Opens in a new window]  and
T. A. Pshenichnikova Open the ORCID record for T. A. Pshenichnikova [Opens in a new window]
A. V. Simonov*
Affiliation:
Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia
O. G. Smirnova
Affiliation:
Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia
M. A. Genaev
Affiliation:
Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia Novosibirsk State University, Novosibirsk, Russia
T. A. Pshenichnikova
Affiliation:
Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia
*
*Corresponding author. E-mail: [email protected]
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Abstract

Leaf pubescence is widespread among higher plants. In bread wheat, a relationship was found between this trait and the efficiency of photosynthetic processes and productivity. In this work, we established the chromosomal localization of the gene for leaf pubescence introgressed from Triticum timopheevii into a bread wheat line 821 and studied its expression in the genetic background of two wheat cultivars differing in genetic control and phenotypic expression of pubescence. To obtain quantitative characteristics of pubescence in cultivars and hybrid populations, the LHDetect2 program was used, which makes it possible to estimate the length and number of trichomes on a leaf fold. A genetic analysis showed the dominant inheritance of the gene. Monosomic analysis F2 was used to establish chromosome localization and investigate the expression of the gene in cultivars Saratovskaya S29 (S29) and Diamant 2 (Dm2). As a result, the gene Hltt, introgressed from T. timopheevii, was identified and localized in the distal region of the long arm of 5A chromosome for the first time. In both F2 populations, the gene reduced the density of trichomes and formed long trichomes, uncharacteristic for the two recipient cultivars S29 and Dm2. A larger number of long trichomes was formed in the genetic background of S29, which carry the bread wheat gene Hl1 and Hl3 for leaf pubescence, than in Dm2. Development of substitution and isogenic lines with the fragment of introgression carrying the gene Hltt will allow determining function and assessing the adaptive significance of the gene more precisely.

Keywords

bread wheatgenetic and monosomic analysesintrogressionleaf pubescencequantitative characteristics of pubescenceTriticum timopheevii
Type
Research Article
Information
Plant Genetic Resources , Volume 19 , Issue 3 , June 2021 , pp. 238 - 244
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of NIAB

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References

Budashkina, E (1988) Cytogenetic study of introgressive disease-resistant common wheat lines. Tag. Ber. Acad. Landwirtsch. Wiss. DDR 206: 209–212.Google Scholar
Dobrovolskaya, OB, Pshenichnikova, TA, Arbuzova, VS, Lohwasser, U, Röder, MS and Börner, A (2007) Molecular mapping of genes determining hairy leaf character in common wheat with respect to other species of the Triticeae. Euphytica 155: 285–293.CrossRefGoogle Scholar
Dorofeev, VF, Filatenko, AA, Migushova, EF, Udachin, RA and Yakubtsiner, MM (1979) Flora of Cultivated Plants of USSR. Vol. 1. Wheat. Leningrad: Kolos Publ. (in Russian).Google Scholar
Doroshkov, AV, Pshenichnikova, TA and Afonnikov, DA (2011) Morphological characterization and inheritance of leaf hairiness in wheat (Triticum aestivum L.) as analyzed by computer-aided phenotyping. Russian Journal of Genetics 47: 739–743.CrossRefGoogle Scholar
Doroshkov, AV, Afonnikov, DA, Dobrovolskaya, OB and Pshenichnikova, TA (2016) Interactions between leaf pubescence genes in bread wheat as assessed by high throughput phenotyping. Euphytica 207: 491–500.CrossRefGoogle Scholar
Doroshkov, AV, Konstantinov, DK, Afonnikov, DA and Gunbin, KV (2019) The evolution of gene regulatory networks controlling Arabidopsis thaliana L. trichome development. BMC Plant Biology 19(Suppl 1): 53.CrossRefGoogle ScholarPubMed
Filatenko, A, Grau, M, Knüpffer, H and Hammer, K (2001) Discriminating characters of diploid wheat species. Proceedings of the 4th International Triticeae Symposium. Cordoba, Spain, 10–12 September 2001. Available at https://wheat.pw.usda.gov/ggpages/GrainTax/Diploid_Wheat_Poster/.Google Scholar
Genaev, MA, Doroshkov, AV, Pshenichnikova, TA, Kolchanov, NA and Afonnikov, DA (2012a) Extracting quantitative characteristics of wheat leaf hairiness using image processing technique. Planta 236: 1943–1954.CrossRefGoogle Scholar
Genaev, MA, Doroshkov, АV, Morozova, EV, Pshenichnikova, TA and Afonnikov, DA (2012b) WheatPGE: a system for analysis of relationships among the phenotype, genotype, and environment in wheat. Russian Journal of Genetics: Applied Research 2: 262–269.CrossRefGoogle Scholar
Hamaoka, N, Yasui, H, Yamagata, Y, Inoue, Y, Furuya, N, Araki, T, Ueno, O and Yoshimura, A (2017) A hairy-leaf gene, BLANKET LEAF, of wild Oryza nivara increases photosynthetic water use efficiency in rice. Rice 10: 20.CrossRefGoogle ScholarPubMed
Jing, H-C, Kornyukhin, D, Kanyuka, K, Orford, S, Zlatska, A, Mitrofanova, OP, Koebner, R and Hammond-Kosack, K (2007) Identification of variation in adaptively important traits and genome-wide analysis of trait–marker associations in Triticum monococcum. Journal of Experimental Botany 58: 3749–3764.CrossRefGoogle ScholarPubMed
Kuspira, J, Maclagana, J, Bhambhan, RN, Sadasivai, RS and Kim, NS (1989) Genetic and cytogenetic analyses of the A genome of Triticum monococcum L. V. Inheritance and linkage relationships of genes determining the expression of 12 qualitative characters. Genome 32: 869–881.CrossRefGoogle Scholar
Lapochkina, IF (2001) Genetic diversity of ‘Arsenal’ collection and its use in wheat breeding. In: Proceedings of international applied sciences conference ‘Genetic Resources of Cultural Plants’, St. Petersburg, pp. 133–135.Google Scholar
Law, CN and Worland, JW (1987) Aneuploidy in wheat and its uses in genetic analysis. In: Lupton, FGH (ed.) Wheat Breeding: Its Scientific Bases. London, New York: Chapman and Hall, pp. 71–107.CrossRefGoogle Scholar
Leonova, IN, Kalinina, NP, Budashkina, EB, Röder, MS and Salina, EA (2001) Comparative molecular and genetic analysis of Triticum aestivum × Triticum timopheevii hybrid lines resistant to leaf rust. EWAC Newsletter. Proceeding of the 11th EWAC Conference, July 24–28, Novosibirsk, Russia, 2000, Ed. T.A. Pshenichnikova, A.J. Worland., pp. 140–143.Google Scholar
Maystrenko, OI (1976) Identification and location of genes controlling leaf pubescence in young plants of bread wheat. Genetika (Moscow) 12: 5–15, (in Russian).Google Scholar
McIntosh, RA, Yamazaki, Y, Dubcovsky, J, Roger, J, Morris, C, Appels, R and Xia, XC (2013) Catalogue of gene symbols for wheat. 12th International Wheat Genetic Symposium, 2013, Yokohama, Japan. http://wheat.pw.usda.gov/GG2/Triticum/wgc/2013/GeneCatalogueIntroduction.pdf.Google Scholar
Pausheva, ZP (1974) Workshop on Plant Cytology. Second edition, revised and enlarged. Moscow: Kolos. (in Russian).Google Scholar
Pshenichnikova, TA, Doroshkov, AV, Simonov, AV, Afonnikov, DA and Börner, A (2017) Diversity of leaf pubescence in bread wheat and relative species. Genetic Resources and Crop Evolution 64: 1761–1773.CrossRefGoogle Scholar
Pshenichnikova, TA, Doroshkov, AV, Osipova, SV, Permyakov, AV, Permyakova, MD, Efimov, VM and Afonnikov, DA (2019) Quantitative characteristics of pubescence in wheat (Triticum aestivum L.) are associated with photosynthetic parameters under conditions of normal and limited water supply. Planta 249: 839–847.CrossRefGoogle ScholarPubMed
Roberts, JJ, Gallun, RL, Patterson, FL and Foster, JE (1979) Effects of wheat leaf pubescence on the Hessian fly. Journal of Economic Entomology 72: 211–214.CrossRefGoogle Scholar
Schillinger, JA and Gallun, RL (1968) Leaf pubescence of wheat as a deterrent to the cereal leaf beetle, Oulema melanopus. Annals of the Entomological Society of America 61: 900–903.CrossRefGoogle Scholar
Sharma, HC and Waines, JG (1994) Inheritance of leaf pubescence in diploid wheat. Journal of Heredity 85: 286–288.CrossRefGoogle Scholar
Taketa, S, Chang, CL, Ishii, M and Takeda, K (2002) Chromosome arm location of the gene controlling leaf pubescence of a Chinese local wheat cultivar ‘Hong-mang-mai’. Euphytica 125: 141–147.CrossRefGoogle Scholar
Vavilov, NI (1987) Theoretical Basis of Breeding. (Collected Works). Moskva. (in Russian): Nauka.Google Scholar