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Genetic diversity in woad (Isatis tinctoria L.) accessions detected by ISSR markers

Published online by Cambridge University Press:  16 March 2011

Luís Rocha ,
Sandra Martins ,
Valdemar Carnide ,
Fernando Braga  and
Carlos Carvalho
Luís Rocha
Affiliation:
Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal
Sandra Martins
Affiliation:
Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal
Valdemar Carnide
Affiliation:
Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal Centre of Genomics and Biotechnology/Institute for Biotechnology and Bioengineering, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal
Fernando Braga
Affiliation:
Centre of Chemistry of Vila Real, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal
Carlos Carvalho*
Affiliation:
Department of Genetics and Biotechnology, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal Centre of Genomics and Biotechnology/Institute for Biotechnology and Bioengineering, University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal
*
*Corresponding author. E-mail: [email protected]
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Abstract

Woad (Isatis tinctoria L.) was introduced in Europe in ancient times to produce indigo, a natural blue pigment used mainly for dyestuff. This species was cultivated in Portugal until the beginning of the 20th century, especially in the inner North and South. A set of nine inter-simple sequence repeat (ISSR) markers generated 177 reproducible fragments, of which 171 were polymorphic. The mean number of fragments/accession was 111, ranging between 100 (Portugal-Coimbra) and 124 (Poland). The total polymorphism observed was 0.3272, the average polymorphism was 0.1784 and the gene differentiation between accessions was 0.4546. Polymorphism ranged between 53.8% (Austria) and 73.1% (Belgium). The genetic relationship among woad accessions was obtained with unweighted pair group method with arithmetic mean dendrogram based on a molecular marker, clearly clustering the woad accessions according to their geographic origin. The genetic diversity observed in this collection shows that there is a considerable potential for its improvement and that ISSR could be used to evaluate intra- and inter-accession similarities in I. tinctoria species.

Keywords

genetic relationshipsinter-simple sequence repeat-PCR markersIsatis tinctoria L.
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 2 , July 2011 , pp. 210 - 213
Copyright
Copyright © NIAB 2011

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