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Genetic diversity and phylogeny analysis of 3-hydroxy 3-methylglutaryl-CoA reductase gene (SmHMGR) in Danshen (Salvia miltiorrhiza Bunge)

Published online by Cambridge University Press:  18 May 2022

Ruihua Ren ,
Fang Liao ,
Deying Kong ,
Yanyan Yin ,
Wei Liu ,
Shaona Teng ,
Jie Feng  and
Guanrong Li Open the ORCID record for Guanrong Li [Opens in a new window]
Ruihua Ren
Affiliation:
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
Fang Liao
Affiliation:
Animal, Plant and Foodstuff Inspection Center, Tianjin Customs, Tianjin 300461, China
Deying Kong
Affiliation:
Technology Center, Chongqing Customs, Chongqing, 401147, China
Yanyan Yin
Affiliation:
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
Wei Liu
Affiliation:
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
Shaona Teng
Affiliation:
Technology Center, Chongqing Customs, Chongqing, 401147, China
Jie Feng
Affiliation:
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
Guanrong Li*
Affiliation:
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China
*
Author for correspondence: Guanrong Li, E-mail: [email protected]
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Abstract

HMGR, 3-hydroxy-3-methylglutaryl-CoA reductase, is a major rate-limiting enzyme in mevalonate (MVA) pathway for isoprenoids and subsequent tanshinone biosynthesis in the Chinese traditional bulk herbal medicine Danshen, Salvia miltiorrhiza, mainly for cardiovascular disorders. In this paper, the genomic SmHMGR genes of 38 cultivated populations of S. miltiorrhiza collected in China were for the first time sequenced to reveal the genetic diversity and phylogeny. The SmHMGR gene was shown to be intron-free, 1650~1659 bp in complete CDS with the majority being 1656 bp, and two unique populations (W-FJLY-V-1 and W-SCHY-W-1) being 1659 and 1650 bp respectively. A total of 103 SNP variation sites were detected with a variation rate of 6.22%, most of which occurred in S. miltiorrhiza f. alba population W-SCHY-W-1; a total of 25 amino acid variation sites were found, of which 19 was in W-SCHY-W-1. The same four populations, W-SCHY-W-1, V-HBAG-V-1, V-JLCC-V-1 and S-NM-V-1 could be discriminated from the remaining 34 by both the SNP fingerprints and the deduced amino acid variation sites. Other or composite DNA markers are needed for better identification. The SmHMGR gene of white flower S. miltiorrhiza f. alba population W-SCHY-W-1 is especially rich in variations and worthy of further studies. Phylogenetic trees based on both the gene and the deduced amino acid sequences showed a very similar two-clade topological structure. This research enriched the content and the genetic means for the molecular identification, genetic diversity and phylogenetic studies of the cultivated S. miltiorrhiza populations, and laid a solid foundation for further related and in-depth investigations.

Keywords

3-hydroxy-3-methylglutaryl-CoA reductase gene (HMGR)cultivated populationgenetic diversityphylogenyS. miltiorrhiza Bunge
Type
Research Article
Information
Plant Genetic Resources , Volume 20 , Issue 1 , February 2022 , pp. 55 - 61
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of NIAB

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Footnotes

*

Equal contributing authors.

References

Basson, ME, Thorsness, M, Finer-Moore, J, Stroud, RM and Rine, J (1988) Structural and functional conservation between yeast and human 3-hydroxy-3-methylglutaryl coenzyme A reductases, the rate-limiting enzyme of sterol biosynthesis. Molecular and Cellular Biology 8, 3797–3808.Google ScholarPubMed
Cao, HM, Wu, J, Jia, LQ and Yang, GL (2017) Progress in study of tanshinone II A's pharmacological effects on cardiovascular system. World Chinese Medicine 12, 1718–1722.Google Scholar
Chen, FY, Guo, R and Zhang, BK (2015) Advances in cardiovascular effects of tanshinone II A. China Journal of Chinese Materia Medica 40, 1649–1653.Google ScholarPubMed
Feng, J, Liao, F, Kong, DY, Liu, W, Yin, YY, Ma, HY, Tang, JH, Ren, RH and Li, GR (2021) Genetic diversity and phylogenetic analysis of rbcL gene of cultivated Salvia miltiorrhiza populations. Highlights of Sciencepaper Online 14, 304–313.Google Scholar
Guo, BL, Lin, S, Feng, YX and Zhao, YJ (2002) Primary research on genetic relationship among main populations of Salvia miltiorrhiza and genuineness of herb. Chinese Traditional and Herbal Drugs 33, 60–63.Google Scholar
Hang, L, Wang, JR, Yang, DF, Shu, ZM and Liang, ZS (2008) Distribution traits of bioactives in different parts of Salvia miltiorrhiza Bunge and Salvia miltiorrhiza Bunge. f. alba. Journal of Northwest A & F University 36, 217–222.Google Scholar
Hung, YC, Pan, TL and Hu, WL (2016) Roles of reactive oxygen species in anticancer therapy with S. miltiorrhiza Bunge. Oxidative Medicine and Cellular Longevity 2016, 1–10. doi: 10.1155/2016/5293284CrossRefGoogle Scholar
Jiang, T, Zhang, MD, Wen, CX, Xie, XL, Tian, W, Wen, SQ, Lu, RK and Liu, LD (2020) Integrated metabolomic and transcriptomic analysis of the anthocyanin regulatory networks in Salvia miltiorrhiza Bge. Flowers. BMC Plant Biology 20, 349.CrossRefGoogle ScholarPubMed
Kai, G, Liao, P, Xu, H, Wang, J, Zhou, CC, Zhou, W, Qi, YP, Xiao, JB, Wang, YL and Zhang, L (2012) Molecular mechanism of elicitor-induced tanshinone accumulation in Salvia miltiorrhiza hairy root cultures. Acta Physiologiae Plantarum 34, 1421–1433.CrossRefGoogle Scholar
Kumar, S, Stecher, G, Li, M, Knyaz, C and Tamura, K (2018) MEGA-X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology & Evolution 35, 1547–1549.CrossRefGoogle ScholarPubMed
Li, H and Guo, YW (2010) Research progress on the identification of Chinese traditional medicines. The 10th Plenary Academic Conference on the Identification of Chinese Traditional Medicines & WHO Identification Methods and Technology Symposium. Xi'an, China Association of Chinese Medicine, pp. 139–143.Google Scholar
Li, ZT, Yang, BJ and Ma, GE (1991) Chemical studies of Salvia miltiorrhiza. f. alba. Acta Pharmaceutica Sinica 26, 209–213.Google ScholarPubMed
Liao, P, Zhou, W, Zhang, L, Wang, J, Yan, XM, Zhang, Y, Zhang, R, Li, L, Zhou, GY and Kai, GY (2009) Molecular cloning, characterization and expression analysis of a new gene encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase from S. miltiorrhiza. Acta Physiologiae Plantarum 31, 565–572.CrossRefGoogle Scholar
Liao, F, Ren, RH, Sun, T, Kong, DY, Liu, JY, Yin, LP and Li, GR (2021) Genetic diversity analysis of cultivated populations of Salvia miltiorrhiza in internal transcribed spacers (ITS). Southwest China Journal of Agricultural Sciences 34, 2093–2099.Google Scholar
Lu, G and Moriyama, EN (2004) Vector NTI, a balanced all-in-one sequence analysis suite. Briefings in Bioinformatics 5, 378–388.CrossRefGoogle ScholarPubMed
Maione, F and Mascolo, N (2016) Danshen and the cardiovascular system: new advances for an old remedy. Seminars in Thrombosis and Hemostasis 42, 321–322.Google ScholarPubMed
Shao, MH, Zhang, YQ and Li, CF (2009) Comparative study on the yield and content of chemical constituent between Salvia miltiorrhiza Bge. And Salvia miltiorrhiza Bge. Var. alba C.Y. Wu et H.W.L. Qilu Pharmaceutical Affairs 28, 24–26.Google Scholar
Shi, Q, Liu, HT, Hao, WY and Xin, Y (2010) Random amplified polymorphic DNA studies of Salvia miltiorrhiza from inner Mongolia. Herald of Medicine 29, 441–442.Google Scholar
Song, ZQ, Wang, JH, Wang, HG, Wang, MM and Xie, YL (2008) Genetic diversity of different eco-geographical populations in Salvia miltiorrhiza revealed by ISSR markers in Shandong China. Acta Ecologica Sinica 28, 5370–5376.Google Scholar
Song, ZQ, Li, XF, Wang, HG and Wang, JH (2010) Genetic diversity and population structure of Salvia miltiorrhiza Bge in China revealed by ISSR and SRAP. Genetica 138, 241–249.CrossRefGoogle ScholarPubMed
Song, ZQ, Wang, JH, Chen, WX and Zhu, HM (2014) Genetic diversity analysis of Salvia miltiorrhiza Bge from different habitats by EST-SSR markers. Journal of Nuclear Agricultural Sciences 28, 193–199.Google Scholar
Song, ZQ, Lin, CC, Xing, PY, Fen, YY, Jin, H, Zhou, CH, Gu, YQ, Wang, JH and Li, XF (2020) A high-quality reference genome sequence of Salvia miltiorrhiza provides insights into tanshinone synthesis in its red rhizomes. The Plant Genome 13, e20041.CrossRefGoogle ScholarPubMed
Tang, XQ, Wang, KC, Chen, X, Wu, J and Yu, BY (2006) AFLP analysis of different cultivars of Salvia miltiorrhiza Bge. Pharmaceutical Biotechnology 13, 182–186.Google Scholar
Wang, H, He, DM, Wang, RT, Song, J, Guo, XH and Yan, ZY (2012) Genetic diversity investigation of cultivated Salvia miltiorrhiza Bge with cpSSR technology. Journal of Anhui Agricultural Sciences 40, 9985–9989.Google Scholar
Wen, CX, Wu, ZM, Tian, W, Liu, M, Zhou, QM and Xie, XL (2007) AFLP analysis of genetic diversity of Salvia miltiorrhiza Bge. Acta Agriculturae Boreali-Sinica 22(S), 122–125.Google Scholar
Zhao, SJ, Hu, ZB, Liu, D and Leung, FCC (2006) Two divergent members of 4-coumarate: coenzyme A ligase from S. miltiorrhiza Bunge: cDNA cloning and functional study. Journal of Integrative Plant Biology 48, 1355–1364.CrossRefGoogle Scholar
Zhou, X, Zhang, ZC, Huang, YB, Xiao, HW, Wu, JJ, Qi, ZC and Wei, YK (2021) Conservation genomics of wild red Sage (Salvia miltiorrhiza) and its endangered relatives in China: population structure and interspecific relationships revealed from 2b-RAD data. Frontiers in Genetics 12, 688323.CrossRefGoogle ScholarPubMed
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