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Genetic diversity analysis using simple sequence repeat markers in soybean

Published online by Cambridge University Press:  16 July 2014

Zhenbin Hu ,
Guizhen Kan ,
Guozheng Zhang ,
Dan Zhang ,
Derong Hao  and
Deyue Yu
Zhenbin Hu
Affiliation:
National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, People's Republic of China
Guizhen Kan
Affiliation:
National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, People's Republic of China
Guozheng Zhang
Affiliation:
National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, People's Republic of China
Dan Zhang
Affiliation:
Department of Agronomy, Henan Agricultural University, Zhengzhou 450002, People's Republic of China
Derong Hao
Affiliation:
Nantong Institute of Agricultural Sciences, Nantong, Jiangsu 226541, People's Republic of China
Deyue Yu*
Affiliation:
National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, People's Republic of China
*
* Corresponding author. E-mail: [email protected]
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Abstract

To evaluate the genetic diversity (GD) of wild and cultivated soybeans and determine the genetic relationships between them, in this study, 127 wild soybean accessions and 219 cultivated soybean accessions were genotyped using 74 simple sequence repeat (SSR) markers. The results of the study revealed that the GD of the wild soybeans exceeded that of the cultivated soybeans. In all, 924 alleles were detected in the 346 soybean accessions using 74 SSRs, with an average of 12.49 alleles per locus. In the 219 cultivated soybean accessions, 687 alleles were detected, with an average of 9.28 alleles per locus; in the 127 wild soybean accessions, 835 alleles were detected, with an average of 11.28 alleles per locus. We identified 237 wild-soybean-specific alleles and 89 cultivated-soybean-specific alleles in the 346 soybean accessions, and these alleles accounted for 35.28% of all the alleles in the sample. Principal coordinates analysis and phylogenetic analysis based on Nei's genetic distance indicated that all the accessions could be classified into two major clusters, corresponding to wild and cultivated soybeans. These results will increase our understanding of the genetic differences and relationships between wild and cultivated soybeans and provide information to develop future breeding strategies to improve soybean yield.

Keywords

genetic diversityprincipal coordinate analysissoybean
Type
Research Article
Information
Plant Genetic Resources , Volume 12 , Issue S1: Special issue on the 3rd International Symposium on “Genomics of Plant Genetic Resources” , July 2014 , pp. S87 - S90
Copyright
Copyright © NIAB 2014 

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