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First report of genome size and ploidy of the underutilized leguminous tuber crop Yam Bean (Pachyrhizus erosus and P. tuberosus) by flow cytometry

Published online by Cambridge University Press:  22 May 2019

Kalidas Pati ,
Fangning Zhang  and
Jacqueline Batley
Kalidas Pati*
Affiliation:
ICAR-Central Tuber Crops Research Institute, Regional Centre, Bhubaneswar, Odisha 751019, India School of Biological Sciences, University of Western Australia, Crawley, WA 6009, Australia
Fangning Zhang
Affiliation:
School of Biological Sciences, University of Western Australia, Crawley, WA 6009, Australia
Jacqueline Batley
Affiliation:
School of Biological Sciences, University of Western Australia, Crawley, WA 6009, Australia
*
*Corresponding author. E-mail: [email protected]
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Abstract

Cytological understanding is an important parameter to understand the genetic architecture of yam bean. The ploidy level and genome size of two cultivated species of yam bean (Pachyrhizus erosus and P. tuberosus) were estimated using flow cytometric analysis of young leaf tissue, with propidium iodide as a fluorescent dye. Six genotypes of P. erosus and three genotypes of P. tuberosus were analysed. Rice (Oryza sativa cv. Nipponbare) and Mung bean (Vigna radiata cv Berken) were used as internal reference standards. Variation of 2C nuclear DNA content among the six P. erousus lines was 4.18%, ranging from 1.17 to 1.22 pg, whereas only 1.8% variation was observed among the three P. tuberosus lines, which ranged from 1.07 to 1.09 pg. Moreover, it was found that the nuclear DNA content of P. tuberosus was lower than that of P. erosus. The result of the flow cytometric analysis showed that all the species were diploid (2n = 2x) and coefficient of variation (CV%) of all the accessions of the two species was less than 3.5%. This is the first report of ploidy analysis and genome size estimation of the leguminous underutilized tuber crop yam bean using flow cytometry. This result will be helpful for yam bean genome sequencing and crop improvement programmes.

Keywords

DNA contentflow cytometrygenome sizePachyrhizus sppploidy levelunderutilized crop
Type
Short Communication
Information
Plant Genetic Resources , Volume 17 , Issue 5 , October 2019 , pp. 456 - 459
Copyright
Copyright © NIAB 2019 

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