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Development of novel polymorphic microsatellite markers in the invasive plant shortawn foxtail (Alopecurus aequalis Sobol.)

Published online by Cambridge University Press:  07 June 2018

Ning Zhao ,
Yanyan Yan ,
Weitang Liu  and
Jinxin Wang Open the ORCID record for Jinxin Wang [Opens in a new window]
Ning Zhao
Affiliation:
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Tai'an 271018, Shandong, People's Republic of China
Yanyan Yan
Affiliation:
State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, Shandong, People's Republic of China
Weitang Liu*
Affiliation:
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Tai'an 271018, Shandong, People's Republic of China
Jinxin Wang*
Affiliation:
Key Laboratory of Pesticide Toxicology and Application Technique, College of Plant Protection, Shandong Agricultural University, Tai'an 271018, Shandong, People's Republic of China
*
*Corresponding author. E-mail: [email protected]; [email protected]
*Corresponding author. E-mail: [email protected]; [email protected]
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Abstract

Shortawn foxtail (Alopecurus aequalis Sobol.) is an invasive and highly troublesome weed species originating from North America that has become widespread across China. Since its proliferation seriously threatens crop production worldwide, understanding its genetic diversity is critical for developing a forecasting system for integrated pest management plans. To accelerate the application of molecular markers in A. aequalis, this study aimed to develop a set of expressed sequence tag-simple sequence repeat (SSR) markers using previous high-throughput sequencing data. In this study, a total of 1411 SSR loci were identified from 95,479 unigenes. Tri-nucleotide repeat motifs were the most abundant type with a frequency of 66.27%, followed by di- (24.95%) and tetra-nucleotide (8.78%). Among the loci, 584 primer pairs were successfully designed for marker development. Subsequently, a subset of 36 primer pairs was randomly selected and synthesized, of which 12 (33.33%) pairs successfully revealed abundant allelic polymorphism. Additionally, to investigate their utility, the genotypes of 160 individuals from 20 natural populations representing diverse wild genotypes of A. aequalis were analysed by using these 12 polymorphic markers. These novel SSR markers developed here are reliable and useful for genetic analysis on this invasive plant and will greatly enrich its genetic resource.

Keywords

Genetic resourcegrass weedmolecular markersimple sequence repeat
Type
Short Communication
Information
Plant Genetic Resources , Volume 16 , Issue 6 , December 2018 , pp. 564 - 567
Copyright
Copyright © NIAB 2018 

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