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Assessment of genetic diversity of Guizotia abyssinica (L.f.) Cass. (Asteraceae) from Ethiopia using amplified fragment length polymorphism

Published online by Cambridge University Press:  01 April 2008

Mulatu Geleta ,
Tomas Bryngelsson ,
Endashaw Bekele  and
Kifle Dagne
Mulatu Geleta*
Affiliation:
Department of Plant Protection Biology, Swedish University of Agricultural Sciences, PO Box 44, SE 230 53 Alnarp, Sweden Department of Biology, Addis Ababa University, PO Box 1176, Addis Ababa, Ethiopia
Tomas Bryngelsson
Affiliation:
Department of Plant Protection Biology, Swedish University of Agricultural Sciences, PO Box 44, SE 230 53 Alnarp, Sweden
Endashaw Bekele
Affiliation:
Department of Biology, Addis Ababa University, PO Box 1176, Addis Ababa, Ethiopia
Kifle Dagne
Affiliation:
Department of Biology, Addis Ababa University, PO Box 1176, Addis Ababa, Ethiopia
*
*Corresponding author. E-mail: [email protected]; E-mail: [email protected]
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Abstract

Seventeen populations of niger (Guizotia abyssinica), representing all regions in Ethiopia where this crop is grown, were investigated using the amplified fragment length polymorphism (AFLP) technique, in order to determine the extent and distribution of its genetic diversity. A total of 539 AFLP loci were scored using seven primer combinations applied to 170 individual plants. Of these, 90% were polymorphic and all the individuals investigated were genetically unique. Despite the fact that most of the variation was within populations, populations were differentiated at a significant level (analysis of molecular variance; P < 0.001). There were no significant differences between populations in relation to the extent and altitude of cultivation. A significant positive correlation was revealed between Nei's standard genetic distance and geographic distance. Cluster analysis and principal coordinate analysis revealed that populations from the same regions were clustered together in most cases. Further collection of niger germplasm from areas underrepresented in gene bank collections is recommended.

Keywords

AFLPAMOVAgenetic diversitygermplasmGuizotia abyssinicanigerpopulation differentiation
Type
Research Article
Information
Plant Genetic Resources , Volume 6 , Issue 1 , April 2008 , pp. 41 - 51
Copyright
Copyright © NIAB 2008

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References

Abebe, M, Yermanos, DM and Bingham, FT (1978) The ecophysiology of noog (Guizotia abyssinica Cass.). African Journal of Agricultural Science 5: 55–66.Google Scholar
Alemaw, G and Teklewold, A (1995) An agronomic and seed-quality evaluation of noog (Guizotia abyssinica Cass.) germplasm from Ethiopia. Plant Breeding 114: 375–376.CrossRefGoogle Scholar
Assefa, K, Merker, A and Hailu, T (2003) Inter simple sequence repeat (ISSR) analysis of genetic diversity in tef [Eragrostis tef (Zucc.) Trotter]. Hereditas 139: 174–183.Google Scholar
Ayres, DR and Ryan, FJ (1997) The clonal and population structure of a rare endemic plant, Wyethia reticulate (Asteraceae): allozyme and RAPD analysis. Molecular Ecology 6: 761–772.CrossRefGoogle Scholar
Baagøe, J (1974) The genus Guizotia (Compositae). A taxonomic revision. Botanisk Tidsskrift 69: 1–39.Google Scholar
Bossdorf, O, Auge, H, Lafuma, L, Rogers, WE, Sicmann, E and Prati, D (2005) Phenotypic and genetic differentiation between native and introduced plant populations. Oecologia 144: 1–11.Google Scholar
Brown-Guedira, GL, Thompson, JA, Nelson, RL and Warburton, ML (2000) Evaluation of genetic diversity of soybean introductions and North American ancestors using RAPD and SSR markers. Crop Science 40: 815–823.Google Scholar
Caetano-Anollés, G and Gresshoff, PM (1994) Staining nucleic acid with silver: an alternative to radioisotopic and fluorescent labeling. Promega Notes Magazine 45: 13–20.Google Scholar
Crow, JF and Aoki, K (1984) Group selection for a polygenic behavioral trait: estimating the degree of population subdivision. Proceedings of the National Academy of Sciences, USA 81: 6073–6077.Google Scholar
Dagne, K (1994) Meiosis in interspecific hybrids and genomic interrelationships in Guizotia Cass. (Compositae). Hereditas 121: 119–129.Google Scholar
Dagne, K (1995) Karyotypes, C-banding and nucleolar numbers in Guizotia (Compositae). Plant Systematics and Evolution 195: 121–135.Google Scholar
Dagne, K (2001) Cytogenetics of new Guizotia Cass. (Compositae), interspecific hybrids pertaining to genomic and phylogenetic affinities. Plant Systematics and Evolution 230: 1–11.CrossRefGoogle Scholar
Dagne, K and Jonsson, A (1997) Oil content and fatty acid composition of seeds of Guizotia Cass. (Compositae). Journal of the Science of Food and Agriculture 73: 274–278.Google Scholar
Dutta, PC, Helmersson, S, Kebedu, E and Alemaw, G (1994) Variation in lipid composition of niger seed (Guizotia abyssinica Cass.) samples collected from different regions in Ethiopia. Journal of the American Oil Chemists Society 71: 839–843.Google Scholar
Ferdinandez, YSN and Coulman, BE (2004) Genetic relationships among smooth bromegrass cultivars of different ecotype detected by AFLP markers. Crop Science 44: 241–247.CrossRefGoogle Scholar
Fichtl, R and Adi, A (1994) Honeybee Flora of Ethiopia. Germany: Margraf Verlag.Google Scholar
Geleta, M, Asfaw, Z, Bekele, E and Teshome, A (2002) Edible oil crops and their integration with the major cereals in North Shewa and South Welo, central Highlands of Ethiopia: an ethnobotanical perspective. Hereditas 137: 29–40.Google Scholar
Geleta, M, Bryngelsson, T, Bekele, E and Dagne, K (2007) Genetic diversity of Guizotia abyssinica (L.f.) Cass. (Asteraceae) from Ethiopia as revealed by random amplified polymorphic DNA (RAPD). Genetic Resources and Crop Evolution 54: 601–614.Google Scholar
Genet, T and Belete, K (2000) Phenotypic diversity in the Ethiopian noug germplasm. African Crop Science Journal 8: 137–143.Google Scholar
Getinet, A and Sharma, SM (1996) Niger (Guizotia abyssinica (L.f.) Cass.): Promoting the Conservation and Use of Underutilized and Neglected Crops. 5. Gatersleben/Rome: Institute of Plant Genetics and Crop Plant Research/International Plant Genetic Resources Institute.Google Scholar
Hamrick, JL and Godt, MJW (1996) Effects of life history traits on genetic diversity in plant species. Philosophical Transactions of the Royal Society of London Series B 351: 1291–1298.Google Scholar
Harlan, JR (1969) Ethiopia: a centre of diversity. Economic Botany 23: 309–314.Google Scholar
Hiremath, SC and Murthy, HN (1988) Domestication of niger (Guizotia abyssinica). Euphytica 37: 225–228.Google Scholar
Hiremath, SC and Murthy, HN (1992) Cytological studies in Guizotia (Asteraceae). Caryologia 45: 69–82.CrossRefGoogle Scholar
Kandel, H and Porter, P (eds) (2002) Niger (Guizotia abyssinica) (L.f.) Cass. Production in northwest Minnesota. University of Minnesota Extension Service.Google Scholar
Lessa, EP (1990) Multidimensional analysis of geographic genetic structure. Systematic Zoology 39: 242–252.Google Scholar
Loveless, MD and Hamrick, JL (1984) Ecological determinants of genetic structure in plant populations. Annual Review of Ecology and Systematics 15: 65–95.Google Scholar
Lynch, M and Milligan, BG (1994) Analysis of population genetic structure with RAPD markers. Molecular Ecology 3: 91–99.Google Scholar
Mantel, NA (1967) The detection of disease clustering and a generalized regression approach. Nucleic Acids Research 21: 1111–1115.Google Scholar
Messmer, MM, Melchinger, AE, Boppenmaier, J, Herrmann, RG and Brunklaus-Jung, E (1992) RFLP analyses of early-maturing European maize germplasm: I. Genetic diversity among flint and dent inbreds. Theoretical and Applied Genetics 83: 1003–1012.Google Scholar
Mohammadi, SA and Prasanna, BM (2003) Analysis of genetic diversity in crop plants – salient statistical tools and considerations. Crop Science 43: 1235–1248.CrossRefGoogle Scholar
Murthy, HN, Hiremath, SC and Salimath, SS (1993) Origin, evolution and genome differentiation in Guizotia abyssinica and its wild species. Theoretical and Applied Genetics 87: 587–592.Google Scholar
Nayakar, NY (1976) Genetic variability and heritability for six quantitative characters in niger (Guizotia abyssinica Cass.). Mysore Journal of Agricultural Science 10: 553–558.Google Scholar
Nei, M (1972) Genetic distance between populations. American Naturalist 106: 283–292.CrossRefGoogle Scholar
Nei, M (1973) Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences, USA 70: 3321–3323.CrossRefGoogle ScholarPubMed
Nei, M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583–590.Google Scholar
Nemomissa, S, Bekele, E and Dagne, K (1999) Self-incompatibility system in Ethiopian populations of Guizotia abyssinica (L.f.) Cass. (niger). Ethiopian Journal of Science 22: 67–88.Google Scholar
Nybom, H (2004) Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Molecular Ecology 13: 1143–1155.Google Scholar
Pavlicek, A, Hrda, S and Flegr, J (1999) FreeTree-Freeware program for construction of phylogenetic trees on the basis of distance data and bootstrap/jackknife analysis of the tree robustness. Application in the RAPD analysis of the genus Frenkelia. Folia Biol-Prague 45: 97–99.Google Scholar
Pradhan, K, Mishra, RC and Paikary, RK (1995) Genetic variability and character association in niger. Indian Journal of Genetics and Plant Breeding 55: 457–459.Google Scholar
Ramadan, MF and Mörsel, J-T (2002) Proximate neutral lipid composition of niger (Guizotia abyssinica Cass.) seed. Czech Journal of Food Science 20: 98–104.Google Scholar
Riley, KW and Belayneh, H (1989) Niger. In: Röbbelen, GDowney, RK and Shri, A (eds) Oil Crops of the World. New York: McGraw Hill, pp. 394–403.Google Scholar
Rohlf, FJ (2000) NTSYSpc, Numerical Taxonomy and Multivariate Analysis System, Version 2.1. Exeter Software, Setauket, New York, USA..Google Scholar
Schneider, S, Roessli, D and Excoffier, L (2000) Arlequin: a software for population genetics data analysis, Version 2.000. Genetics and Biometry Lab, Department of Anthropology, University of Geneva..Google Scholar
Shim, SI and Jørgensen, RB (2000) Genetic structure in cultivated and wild carrots (Daucus carota L.) revealed by AFLP analysis. Theoretical and Applied Genetics 101: 227–233.CrossRefGoogle Scholar
Vos, P, Hogers, R, Bleeker, M, Reijans, M, van de Lee, T, Hornes, M, Frijters, A, Pot, J, Peleman, J, Kuiper, M and Zabeau, M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23: 4407–4414.CrossRefGoogle ScholarPubMed
Wright, S (1951) The genetical structure of populations. Annals of Eugenics 15: 323–354.CrossRefGoogle ScholarPubMed
Yeh, FC and Boyle, TJB (1997) Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belgium Journal of Botany 129: 157.Google Scholar
Zeven, AC and de Wet, JMJ (1982) Dictionary of Cultivated Plants and their Regions of Diversity. Dehra Dun, India: International Book Distributors.Google Scholar