This article examines the unusual commemorative Office of the Virgin from Barking Abbey in a fifteenth-century book of hours, Cambridge, University Library, Dd.12.56 (hereafter Dd.12.56). As already evident in the Barking Ordinal, the nuns of this Abbey venerated Mary, one of their patron saints, with a weekly full three-nocturn, twelve-lesson Matins service. Dd.12.56, however, has only recently been linked to Barking Abbey and drawing on new material in this manuscript in correlation with others from the Abbey, I argue that the Barking nuns compiled a unique series of readings and responsories to honour Mary. The progression through the three nocturns of Matins articulates a Marian theology that intersects with the nuns’ self-understanding and I demonstrate that they carefully crafted the lesser hours to highlight specific times of day and to complement Marian hymns sung at Barking. The accepted belief that books of hours were for personal devotion obscures the possibility that such books reflect communal liturgical practices, potentially serving multiple purposes within a monastic setting. Although Dd.12.56 dates from the fifteenth century, it may testify to a much older liturgical practice, which originated in the twelfth century when the three-nocturn format was still prevalent in Benedictine use and before it was largely replaced by a two-nocturn format in the thirteenth century. Benedictine tradition offered individual monastic houses the opportunity to craft this service. Dd.12.56 stands as a new and important testimony to the rich and imaginative ways in which the Barking community created, collated and curated materials that they steeped in their minds and hearts.