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Fossil macromolecules from cephalopod shells: characterization, immunological response and diagenesis

Published online by Cambridge University Press:  08 February 2016

P. Westbroek ,
P. H. van der Meide ,
J. S. van der Wey-Kloppers ,
R. J. van der Sluis ,
J. W. de Leeuw  and
E. W. de Jong
P. Westbroek
Affiliation:
Department of Biochemistry, State University Leiden, P.O. Box 9505, 2300 RA Leiden, The Netherlands
P. H. van der Meide
Affiliation:
Department of Biochemistry, State University Leiden, P.O. Box 9505, 2300 RA Leiden, The Netherlands
J. S. van der Wey-Kloppers
Affiliation:
Department of Biochemistry, State University Leiden, P.O. Box 9505, 2300 RA Leiden, The Netherlands
R. J. van der Sluis
Affiliation:
Department of Biochemistry, State University Leiden, P.O. Box 9505, 2300 RA Leiden, The Netherlands
J. W. de Leeuw
Affiliation:
Department of Chemistry and Chemical Engineering, University of Technology, Delft, The Netherlands
E. W. de Jong
Affiliation:
Department of Biochemistry, State University Leiden, P.O. Box 9505, 2300 RA Leiden, The Netherlands
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Abstract

The EDTA-soluble fractions extracted from rostra of two late Cretaceous belemnites (Cephalopoda), Gonioteuthis and Belemnitella, were divided into an humic acid and a fulvic acid fraction (the latter was absent in Belemnitella). The extracts are compared with preparations from shells of two recent cephalopods, Nautilus pompilius and Sepia officinalis. Use was made of immunology, amino acid analysis, pyrolysis mass spectrometry and some other techniques.

The fulvic acid fraction of Gonioteuthis, a mixed peptide-like saccharide-like substance, produced confluent immunodiffusion patterns with an EDTA-soluble Nautilus extract against anti-Nautilus rabbit serum. The humic acid of Gonioteuthis did not contain D-alloisoleucine and its amino acid composition was very similar to that of the EDTA-insoluble fraction of Nautilus. This humic acid was enriched in polyphenol, which may be due to chemical reaction of peptides and carbohydrates during diagenesis. It is concluded that both fractions of Gonioteuthis are original belemnite materials that have undergone only minor alterations during diagenesis.

This is an exploratory study of biochemical compounds derived from fossils, with particular emphasis on immunological methods.

Type
Research Article
Information
Paleobiology , Volume 5 , Issue 2 , Spring 1979 , pp. 151 - 167
Copyright
Copyright © The Paleontological Society 

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References

Literature Cited

Carter, P. W. and Mitterer, R. M. 1978. Amino acid composition of organic matter associated with carbonate and non-carbonate sediments. Geochim. Cosmochim. Acta. 42:1243–1251.CrossRefGoogle Scholar
Crenshaw, M. A. 1972. The soluble matrix from Mercenaria mercenaria shell. Biomineralization. 6:6–11.Google Scholar
Degens, E. T. 1976. Molecular mechanisms on carbonate, phosphate, and silica deposition in the living cell. Top. Curr. Chem. 64:1–112.CrossRefGoogle ScholarPubMed
Degens, E. T., Spencer, D. W., and Parker, R. H. 1967. Paleobiochemistry of molluscan shell proteins. Comp. Biochem. Physiol. 20:553–579.CrossRefGoogle Scholar
Dische, Z. 1955. New color reactions for the determination of sugars in polysaccharides. Methods Biochem. Anal. 2:313–358.CrossRefGoogle ScholarPubMed
Gottschalk, A. 1966. Glycoproteins, their Composition, Structure and Function. 628 pp. BBA Library. 5. Elsevier; Amsterdam.Google Scholar
Hare, P. E. and Hoering, T. C. 1977. The organic constituents of fossil mollusc shells. Carnegie Inst. Wash. Year Book. 78:625–631.Google Scholar
Hoering, T. C. 1973 (1972). Comparison of melanoidin and humic acid. Carnegie Inst. Wash. Year Book. 72:682–690.Google Scholar
de Jong, E. W., Westbroek, P., Westbroek, J. F., and Bruning, J. W. 1974. Preservation of antigenic properties in macromolecules over 70 myr old. Nature. 252:63.CrossRefGoogle Scholar
Kabat, E. A. and Mayer, M. M. 1964. In: Experimental Immunochemistry. 2nd ed. Pp. 85. Thomas C. C.; Springfield, Illinois.Google Scholar
Krampitz, G., Weise, K., Potz, A., Engels, J., Samata, T., Becker, K., Hedding, M., and Flajs, G. 1977. Calcium-binding peptide in dinosaur egg shells. Naturwissenschaften. 64:583.CrossRefGoogle Scholar
Lowry, O. H., Rosenbrough, N. J., Farr, A. L., and Randall, R. J. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193:265–276.CrossRefGoogle ScholarPubMed
van der Meide, P. H., Westbroek, P., de Jong, E. W., de Leeuw, J. W., and Meuzelaar, H. L. C. 1979. Characterization of macromolecules from fossil shells by immunology and Curie-point pyrolysis mass spectrometry. Proc. 3rd Int. Symp. Mechanisms Mineral. Invertebr. Plants (Japan, 1977). In press.Google Scholar
Meuzelaar, H. L. C., Haider, K., Nagar, B. R., and Martin, J. P. 1977. Comparative studies of pyrolysis-mass spectra of melanins, model phenolic polymers, and humic acids. Geoderma. 17:239–252.CrossRefGoogle Scholar
Meuzelaar, H. L. C., Kistemaker, P. G., Eshuis, W., and Boerboom, H. A. J. 1976. Automated pyrolysis mass spectrometry; application to the differentiation of microorganisms. Adv. Mass Spectrom. 78:1452–1456.Google Scholar
Nawrot, C. F., Campbell, D. J., Schroeder, J. K., and Van Valkenburg, M. 1976. Dental phosphoprotein-induced formation of hydroxylapatite during in vitro synthesis of amorphous calcium phosphate. Biochemistry. 15(16):3445–3449.CrossRefGoogle ScholarPubMed
Park, J. F. and Johnson, M. J. 1949. A submicrodetermination of glucose. J. Biol. Chem. 181:149–151.CrossRefGoogle ScholarPubMed
Posthumus, M. A., Boerboom, H. A. J., and Meuzelaar, H. L. C. 1974. Analysis of biopolymers by Curie-point pyrolysis in direct combination with low voltage electron impact ionization mass spectrometry. Adv. Mass Spectrom. 6:397–402.Google Scholar
Schroeder, R. A. and Bada, J. L. 1976. A review of the geochemical applications of the amino acid racemization reaction. Earth Sci. Rev. 12:347–391.CrossRefGoogle Scholar
Speath, C. 1975. Zur Frage der Schwimmverhältnisse bei Belemniten in Abhängigkeit vom Primärgefüge der Hartteile. Paläontol. Z. 49(3):321–331.CrossRefGoogle Scholar
Weiner, S. and Hood, L. 1975. Soluble protein of the organic matrix of mollusk shells: a potential template for shell formation. Science. 190:987–989.CrossRefGoogle ScholarPubMed
Weiner, S., Lowenstam, H. A., and Hood, L. 1976. Characterization of 80-million-year-old mollusc shell proteins. Proc. Natl. Acad. Sci. USA. 73(8):2541–2545.CrossRefGoogle Scholar
Weiner, S., Lowenstam, H. A., and Hood, L. 1977. Discrete molecular weight components of the organic matrices of mollusc shells. J. exp. mar. Biol. Ecol. 30:45–51.CrossRefGoogle Scholar
Wyckoff, R. W. G. 1972. The Biochemistry of Animal Fossils. VIII + 152 pp. Scientechnica; Bristol.Google Scholar