The Amazonian manatee Trichechus inunguis is a freshwater sirenian categorized as Vulnerable on the IUCN Red List. Since 2008, the Instituto Nacional de Pesquisas da Amazônia (National Institute of Amazonian Research) of Brazil with the support of the Associação Amigos do Peixe-boi (Friends of the Manatee Association) has been releasing rehabilitated manatees in the wild as a strategy for the conservation of this endemic species. The programme's indicators of success are: (1) exploration of the area by the released manatees, (2) selection of suitable habitats, (3) survival over at least one complete river flood pulse, (4) interaction with wild manatees, and (5) absence of contact with humans. Because of the difficulty of detecting manatees, breeding is not included in this evaluation.

During 2008–2019, a total of 35 rehabilitated manatees were released in the Brazilian Amazon. Twenty-two (63%) with a VHF transmitter were monitored by trained ex-hunters for up to 752 days; 74% of the monitored manatees adapted successfully to the wild. To evaluate the health of the released manatees, recaptures are attempted during the dry season, involving biologists, veterinarians and fishers. Individuals are located using the VHF signal and, if the location is suitable, such as a small, shallow lake, the individual is surrounded by specially designed nets. Once captured, the manatee is transferred on a stretcher to the margin of the lake for collection of biometric data and blood and faecal samples.

Four manatees have been recaptured 6–18 months after release (one in 2016, one in 2017, and two in 2019). They had a mean increase of 45 kg in body mass and 10 cm in total length. One of the manatees recaptured in 2019 was Baré, a female rescued as an orphan calf and kept in captivity for 16 years. After 18 months in the wild, she had increased by 12 cm in length and gained 106 kg. The large abdominal volume and vulvar oedema suggested an advanced pregnancy, later confirmed by serum progesterone analysis. Baré's pregnancy, and the participation of local people in the protection and monitoring of the manatees, confirms the success of the programme.

The lessons learnt will support future management planning for Amazonian manatees. In July 2021, Instituto Nacional de Pesquisas da Amazônia and Associação Amigos do Peixe-boi released 13 rehabilitated manatees, the largest group to date. Our experience shows that a combination of releases and environmental education activities is an effective conservation tool for the Amazonian manatee.