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Repeated sequence markers in pea (Pisum sativum)

Published online by Cambridge University Press:  01 September 1997

L. TURNER
Affiliation:
Department of Applied Genetics, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
T. H. N. ELLIS
Affiliation:
Department of Applied Genetics, John Innes Centre, Colney Lane, Norwich NR4 7UH, UK
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Abstract

Multiple-marker techniques based on PCR are highly informative in genetic analysis. The bulk of many genomes is comprised of repeated sequences, and thus anonymous markers derived from genomic DNA are likely to correspond to repeated DNA sequences. Here we show that repeated-sequence-derived markers can provide information similar in quality to that of other PCR-based markers. Presumably this reflects the abundance of this repeat type in anonymous PCR markers such as RAPDs or AFLPs.

Type
Research Article
Copyright
© Trustees of the New Phytologist 1997

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