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Molecular determination of genetic variation in Pisolithus isolates from a defined region in New South Wales, Australia

Published online by Cambridge University Press:  01 January 1998

I. C. ANDERSON ,
S. M. CHAMBERS  and
J. W. G. CAIRNEY
I. C. ANDERSON
Affiliation:
Mycorrhiza Research Group, School of Science, University of Western Sydney (Nepean), PO Box 10, Kingswood, NSW 2747, Australia
S. M. CHAMBERS
Affiliation:
Mycorrhiza Research Group, School of Science, University of Western Sydney (Nepean), PO Box 10, Kingswood, NSW 2747, Australia
J. W. G. CAIRNEY
Affiliation:
Mycorrhiza Research Group, School of Science, University of Western Sydney (Nepean), PO Box 10, Kingswood, NSW 2747, Australia
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Abstract

Sixty-two isolates of Pisolithus were obtained from a carpophore population collected from a 90-km2 area of New South Wales, Australia. Random amplified polymorphic DNA (RAPD) analysis was used initially to screen isolates for variation, and restriction fragment length polymorphism (RFLP) analysis of the internal transcribed spacer (ITS) region and ITS sequence analysis were subsequently performed on selected isolates. ITS–RFLP and ITS sequence analysis indicated that two isolates (LJ07 and WM01) from different sites were considerably different from all other isolates, a strict consensus tree generated by bootstrap analysis of sequence data strongly suggesting that isolates LJ07 and WM01 represent a separate Pisolithus species. Although the general morphology of carpophores and basidiospores of the majority of collected isolates was consistent with previous descriptions of P. tinctorius (Pers.) Coker and Couch, basidiospore characteristics suggest that isolates LJO7 and WMO1 might represent a Pisolithus species distinct from P. tinctorius and the previously described Australian species, P. microcarpus (Cooke & Massee) G. Cunn.

Keywords

Pisolithus tinctorius (Pers.) Coker and CouchPisolithus microcarpus (Cke. and Mass.) Cunn.RAPDITS–RFLPITS sequencesribosomal DNA
Type
Research Article
Information
The New Phytologist , Volume 138 , Issue 1 , January 1998 , pp. 151 - 162
Copyright
Trustees of the New Phytologist 1998

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