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Cytolocalization of glycogen, starch, and other insoluble polysaccharides during ontogeny of Paxillus involutusBetula pendula ectomycorrhizas

Published online by Cambridge University Press:  01 October 1998

M. N. JORDY
Affiliation:
Laboratoire de Biologie Forestière, Université Henri Poincaré, Nancy I, BP 239, 54506 Vandoeuvre-Lès-Nancy, France
S. AZÉMAR-LORENTZ
Affiliation:
Laboratoire de Biologie Forestière, Université Henri Poincaré, Nancy I, BP 239, 54506 Vandoeuvre-Lès-Nancy, France
A. BRUN
Affiliation:
Laboratoire de Biologie Forestière, Université Henri Poincaré, Nancy I, BP 239, 54506 Vandoeuvre-Lès-Nancy, France
B. BOTTON
Affiliation:
Laboratoire de Biologie Forestière, Université Henri Poincaré, Nancy I, BP 239, 54506 Vandoeuvre-Lès-Nancy, France
J. C. PARGNEY
Affiliation:
Laboratoire de Biologie Forestière, Université Henri Poincaré, Nancy I, BP 239, 54506 Vandoeuvre-Lès-Nancy, France
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Abstract

The Paxillus involutus (Fries) Karsten–Betula pendula Roth association was studied during the early stages of formation. Cytological studies revealed fungal colonization behind the root cap and gradually around the entire root apex. Ultrastructural investigations were carried out and insoluble polysaccharide distribution was followed. The density of starch grains increased in plant cells especially after 4 d of contact between the two partners, but later on decreased strongly in the root cap. Large amounts of glycogen were revealed in the hyphae in certain mycorrhizal regions after 6 d of contact: in the Hartig net, in the inner sheath but only near the net, and all along the outer sheath surrounding the mycorrhiza. Thickenings of the epidermal cell walls were detected as early as 2 d after contact and then varied according to the distance from the root tip. Such polysaccharide distributions are assumed to show a transfer of carbohydrates from the root to the fungus and are discussed in terms of carbon requirements for both partners.

Type
Research Article
Copyright
© Trustees of New Phytologist 1998

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